Economic challenges and problems related to natural resources management as a consequence of human capital accumulation

The current study considers the evolution of the concept of human capital over time. The theory of human capital was shaped from research results obtained by foreign economists. Thus, it covers many independent existing aspects that should also be studied independently. This article draws special attention to the impact of abundant natural resources on the accumulation of human capital. The article analyzes modern theories through the lens of an idea that economic growth is dependent upon the abundance of natural resources. The institutional factor also plays a role in this relationship, so classic economic theories were considered as well.El presente estudio considera la evolución del concepto de capital humano a lo largo del tiempo. La teoría del capital humano se formó a partir de los resultados de la investigación obtenidos por economistas extranjeros. Por lo tanto, cubre muchos aspectos independientes que deberían estudiarse también de forma independiente. Este artículo llama especialmente la atención sobre el impacto de los abundantes recursos naturales en la acumulación de capital humano. El artículo analiza las teorías modernas a través de la idea de que el crecimiento económico depende de la abundancia de recursos naturales. El factor institucional también juega un papel en esta relación, por lo que también se consideraron las teorías económicas clásicas

Elaborating of a cryoconservation protocol of blood vessels for the tissue-specific matrices

Purpose: to create a protocol for complex cryoconservation of blood vessels in polydimethylsiloxane to create tissue-specific matrices. Material and Methods. The material ofthe study was the iliac arteries from postmortem donors, seized in the multi-organ donation, rejected for unclaimed and disposed of later. Polydimethylsiloxane was used as a coolant (PDMS) with a viscosity of 5-25 centistokes (Spectroplast, Russia). Bench testing of cooling and heating ofthe blood vessel in PDMS, testing of heating and cooling of PDMS up to operating temperatures (-80°C) in different ways and variants of its thermal insulation, assessment of different cooling rates ofthe vessel during cryoconservation, modeling of 3 types of working chambers for cryoconservation, sterilization and defrosting of up to 10 vessels, modeling of cryoconservation and thawing of vessels in PDMS under different conditions, selection of substrates from silicone and plastic for uniform fixation of vessels which are of size or size in the working chambers or without them. Results. The developed protocol allows cryopreserved from 1 to 10 or more vessels of all basic sizes both immediately after removal and after transportation to the laboratory, to vary the cooling rate from 1.5 to 300°C/min and above, to be used with different levels of laboratory equipment, as with liquid nitrogen, dry ice, and only with a medical freezer. Conclusion. The developed protocol allows to apply it in cases of complex cryoconservation of blood vessels to create tissue-specific matrices and can be recommended for preclinical testing.</p