3,174 research outputs found
Measuring cosmological bulk flows via the kinematic Sunyaev-Zeldovich effect in the upcoming cosmic microwave background maps
We propose a new method to measure the possible large-scale bulk flows in the
Universe from the cosmic microwave background (CMB) maps from the upcoming
missions, MAP and Planck. This can be done by studying the statistical
properties of the CMB temperature field at many X-ray cluster positions. At
each cluster position, the CMB temperature fluctuation will be a combination of
the Sunyaev-Zeldovich (SZ) kinematic and thermal components, the cosmological
fluctuations and the instrument noise term. When averaged over many such
clusters the last three will integrate down, whereas the first one will be
dominated by a possible bulk flow component. In particular, we propose to use
all-sky X-ray cluster catalogs that should (or could) be available soon from
X-ray satellites, and then to evaluate the dipole component of the CMB field at
the cluster positions. We show that for the MAP and Planck mission parameters
the dominant contributions to the dipole will be from the terms due to the SZ
kinematic effect produced by the bulk flow (the signal we seek) and the
instrument noise (the noise in our signal). Computing then the expected
signal-to-noise ratio for such measurement, we get that at the 95 % confidence
level the bulk flows on scales >100h^{-1} Mpc can be probed down to the
amplitude of km/sec with the MAP data and down to only 30 km/sec with
the Planck mission.Comment: Astrophysical Journal Letters, in pres
Load fluctuations drive actin network growth
The growth of actin filament networks is a fundamental biological process
that drives a variety of cellular and intracellular motions. During motility,
eukaryotic cells and intracellular pathogens are propelled by actin networks
organized by nucleation-promoting factors, which trigger the formation of
nascent filaments off the side of existing filaments in the network. A Brownian
ratchet (BR) mechanism has been proposed to couple actin polymerization to
cellular movements, whereby thermal motions are rectified by the addition of
actin monomers at the end of growing filaments. Here, by following
actin--propelled microspheres using three--dimensional laser tracking, we find
that beads adhered to the growing network move via an object--fluctuating BR.
Velocity varies with the amplitude of thermal fluctuation and inversely with
viscosity as predicted for a BR. In addition, motion is saltatory with a broad
distribution of step sizes that is correlated in time. These data point to a
model in which thermal fluctuations of the microsphere or entire actin network,
and not individual filaments, govern motility. This conclusion is supported by
Monte Carlo simulations of an adhesion--based BR and suggests an important role
for membrane tension in the control of actin--based cellular protrusions.Comment: To be published in PNA
Identification of a membrane binding peptide in the envelope protein of MHV coronavirus
Coronaviruses (CoVs) are enveloped, positive sense, single strand RNA viruses that cause respiratory, intestinal and neurological diseases in mammals and birds. Following replication, CoVs assemble on intracellular membranes including the endoplasmic reticulum Golgi intermediate compartment (ERGIC) where the envelope protein (E) functions in virus assembly and release. In consequence, E potentially contains membrane-modifying peptides. To search for such peptides, the E coding sequence of Mouse Hepatitis Virus (MHV) was inspected for its amino acid conservation, proximity to the membrane and/or predicted amphipathic helices. Peptides identified in silico were synthesized and tested for membrane-modifying activity in the presence of giant unilamellar vesicles (GUVs) consisting of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC), sphingomyelin and cholesterol. To confirm the presence of membrane binding peptides identified in the context of a full-length E protein, the wild type and a number of mutants in the putative membrane binding peptide were expressed in Lenti-X-293T mammalian and insect cells, and the distribution of E antigen within the expressing cell was assessed. Our data identify a role for the post-transmembrane region of MHV E in membrane binding
A fusion peptide in the spike protein of MERS coronavirus
Coronaviruses represent current and emerging threats for many species, including humans. Middle East respiratory syndrome-related coronavirus (MERS-CoV) is responsible for sporadic infections in mostly Middle Eastern countries, with occasional transfer elsewhere. A key step in the MERS-CoV replication cycle is the fusion of the virus and host cell membranes mediated by the virus spike protein, S. The location of the fusion peptide within the MERS S protein has not been precisely mapped. We used isolated peptides and giant unilamellar vesicles (GUV) to demonstrate membrane binding for a peptide located near the N-terminus of the S2 domain. Key residues required for activity were mapped by amino acid replacement and their relevance in vitro tested by their introduction into recombinant MERS S protein expressed in mammalian cells. Mutations preventing membrane binding in vitro also abolished S-mediated syncytium formation consistent with the identified peptide acting as the fusion peptide for the S protein of MERS-CoV
The Portrayal of Complementary and Alternative Medicine in Mass Print Magazines Since 1980
Objectives: The objectives of this study were to examine and describe the portrayal of complementary and alternative medicine (CAM) in mass print media magazines.
Design: The sample included all 37 articles found in magazines with circulation rates of greater than 1 million published in the United States and Canada from 1980 to 2005. The analysis was quantitative and qualitative and included investigation of both manifest and latent magazine story messages.
Results: Manifest analysis noted that CAM was largely represented as a treatment for a patient with a medically diagnosed illness or specific symptoms. Discussions used biomedical terms such as patient rather than consumer and disease rather than wellness. Latent analysis revealed three themes: (1) CAMs were described as good but not good enough; (2) individualism and consumerism were venerated; and (3) questions of costs were raised in the context of confusion and ambivalence
Mixed-state twin observables
Twin observables, i.e. opposite subsystem observables A+ and A- that are
indistinguishable in measurement in a given mixed or pure state W, are
investigated in detail algebraicly and geometrically. It is shown that there is
a far-reaching correspondence between the detectable (in W) spectral entities
of the two operators. Twin observables are state-dependently quantum-logically
equivalent, and direct subsystem measurement of one of them ipso facto gives
rise to the indirect (i.e. distant) measurement of the other. Existence of
nontrivial twins requires singularity of W. Systems in thermodynamic
equilibrium do not admit subsystem twins. These observables may enable one to
simplify the matrix representing W.Comment: 13 page
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Oligonucleotide compound and method for treating nidovirus infections
A method and oligonucleotide compound for inhibiting replication of a nidovirus in virus-infected animal cells are disclosed. The compound (i) has a nuclease-resistant backbone, (ii) is capable of uptake by the infected cells, (iii) contains between 8-25 nucleotide bases, and (iv) has a sequence capable of disrupting base pairing between the transcriptional regulatory sequences in the 5′ leader region of the positive-strand viral genome and negative-strand 3′ subgenomic region. In practicing the method, infected cells are exposed to the compound in an amount effective to inhibit viral replication
Transnational knowledge in social work programs : challenges and strategies within assisted voluntary return and reintegration support
Residents\u27 perceptions of smart energy metres
Smart metres are a form of expert system with performance features beyond energy‐consumption record keeping, to include monitoring, analysing, and estimating metre readings. Although smart metres have great capabilities, this technology is still in its infancy in many developing countries, and little is known about the kinds of risks associated with smart metres from residents\u27 perspectives. This research therefore aims to fill this gap by examining the influence of four different types of perceived risk on residents\u27 intentions to use smart metres in Jordan. By following a quantitative approach, 242 survey responses were tested by using structural equation modelling–partial least squares. The statistical results indicated that perceived security and technical risks have a significant and negative impact on residents\u27 intentions to use smart metres. However, perceived privacy and health risks, surprisingly, were found to have no significant negative influence on intention to use. Theoretical and practical implications are indicated, and directions of future research are subsequently specified
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