Diagnostic and prognostic significance of some inflammatory serum proteins in patients with precancerous diseases and cervical cancer

Background: The high social significance of cervical cancer, the shortcomings of the performed cervical screening is prerequisites for research in the field of mproving the diagnosis of this disease. We hypothesized that the systemic level of some inflammatory proteins could be used as a diagnostic criterion for cervical cancer.The aim of the study was to study the level of some vascular-inflammatory markers in the blood serum in patients with precancerous diseases and cervical cancer in order to improve their diagnosis and also to identify markers for predicting an unfavorable outcome in patients with cervical cancer.Materials and methods. A non-randomized prospective controlled study was carried out, the participants of which were patients with the diagnosis of cervical cancer (n = 49) and cervical intraepithelial neoplasia of the III degree (n = 13). The control group included 15 relatively healthy women. The following spectrum of inflammatory proteins was determined in blood serum by flow cytometry using the Human Vascular Inflammation Panel 1: myoglobin, calprotectin, lipocalin A, matrix metal peroxidase 2, osteopontin, myeloperoxidase, serum amyloid A, protein 4, which binds insulin-like growth factor cell-cell adhesion 1, vascular cell adhesion molecule, matrix metalloperoxidase 9, cystatin C. Statistical analysis was carried out by calculating the Mann-Whitney test with Bonferroni’s correction. The model was created using binary logistic regression to diagnose cervical cancer.Results. In the intergroup comparison of the protein`s spectrum in the blood serum, no significant differences were obtained. However, using the binary logistic regression method, an equation was drawn up to calculate the diagnostic coefficient of cervical cancer, which allows diagnosing cervical cancer with an accuracy of 82%, and in terms of information content is not inferior to cytological diagnostics. The developed coefficient can be used to predict an unfavorable outcome of cervical cancer after 1 year from the moment of diagnosis. Conclusion. The developed diagnostic coefficient makes it possible to  diagnose cervical cancer with high accuracy and can be used to predict cervical cancer

Conversion of the Organic Matter of Domanic Shale and Permian Bituminous Rocks in Hydrothermal Catalytic Processes

© 2017 American Chemical Society. Comparative studies of hydrothermal transformation of organic matter samples of bituminous rocks from Domanic and Permian deposits of the Tatarstan Republic (Russian Federation) have been carried out. The experiments have been taken at a temperature of 300 °C in a vapor-gas carbon dioxide environment, with a content of 30% water in the reactionary system and an initial pressure of carbon dioxide of 2 MPa. It is shown that the distinguishing features of the organic matter of the analyzed species according to thermal analysis data are most prominent in the loss of its mass at various temperature intervals. Also, changes take place in group and structural composition and hydrocarbon composition, which have an impact on the composition of the initial products of experiments. Hydrothermal effects on the Domanic rock sample result in the destruction of structural polymer fragments of kerogen. It results in an increase in the contents of asphaltenes and their modified structures in the form of carben-carboids, insoluble in solvents characteristic of asphaltenes, thus reducing the relative content of saturated hydrocarbons in the products of the experiment. Under similar hydrothermal conditions, changes in the physical composition of the Permian rock are less significant. For the investigation of activation processes of the transformation of organic matter of the Permian rock, an experiment was conducted using catalysts. As catalysts, a composition of oil-soluble iron(II), cobalt(II), and copper(II) carboxylates with an additive of propanol was used. The use of the catalyst has increased the output of light fractions and reduced the content of resins and asphaltenes as part of Permian rock

対人関係の光と影 : 「絆」の形成、拒絶、そして崩壊の社会心理学的研究

© 2015 Springer Science+Business Media New York. The feasibility of applying thermal analysis to study of oil-containing rocks and organic matter is reviewed. Using heavy crudes from the Ashal'cha and Mordovo-Karmal fields, the potential for analysis of the effectiveness of iron-containing precursors of aquathermolysis catalysts is demonstrated with use of data derived from thermal analysis. The thermal effects detected in the presence of the catalyst precursor, as compared with the original sample, reflect decomposition of the catalyst precursor and degradation processes for the components of the crude oil activated by the catalyst. It is shown that use of thermal analysis is feasible for preliminary selection or optimization of catalyst compositions for in-situ upgrading of crude oils, taking into account the activity of the catalyst relative to certain components of the crude in a specific temperature range

EPR study of hydrocarbon generation potential of organic-rich domanik rocks

© Kazan Federal University (KFU). The objects of investigation were samples of domanik formation rocks of Berezovskaya area of Romashkinskoye oil field. Content of Mn 2+ , SO 3 − , SO 2 − ions, vanadyl-ions and free radicals has been estimated with the X-band (9.43 GHz) electron paramagnetic resonance (EPR) spectroscopy. Composition of rock and extracted from it bitumen has been studied with methods of thermal analysis, X-ray-structural analysis and gas chromatography. Significant differences in mineralogical composition and content of organic matter were revealed in samples taken from adjacent intervals. Pyrolytic experiments have been conducted to simulate kerogen maturation and petroleum generation. The increasing number of free radicals was registered in all samples after pyrolysis in a hydrogen atmosphere at 350°C. A new organic free radical C350 was registered after pyrolysis. It is suggested that domanik source rocks have not fully realized their hydrocarbon generation potential

Road bitumen's based on the vacuum residue of heavy oil and natural asphaltite: Part II–physical and mechanical properties

© 2017 Taylor & Francis Group, LLC. This work is devoted to studying the possibility of producing bitumen for road construction by using vacuum residue > 420°C of heavy oil of the Ashal'chinskoe field in and natural asphaltite Spiridonovskoe field from Permian deposits in Tatarstan. The effect of natural asphaltite as a solid disperse phase element on the structural and group composition of the residual heavy oil product and its malacometrical qualities (penetration, extensibility, softening point, resistance to aging and adhesion) are revealed. Samples, compounded bitumen production, were carried out by introducing the required amount of the shredded asphaltite to deasphaltizat vacuum residue of heavy oil and heating their mixture to 220°С with vigorous stirring. Changes in the composition and physical and chemical properties of deasphalting the residual heavy oil product, associated with the amount of injected asphaltite, showed the possibility of production of modified bitumen with better adhesion properties that correspond to road bitumen

Road bitumen's based on the vacuum residue of heavy oil and natural asphaltite: Part I–chemical composition

© 2017 Taylor & Francis Group, LLC. This work is devoted to studying the possibility of producing bitumen for road construction by using vacuum residue > 420°C of heavy oil of the Ashal'chinskoe field in and natural asphaltite Spiridonovskoe field from Permian deposits in Tatarstan. The effect of natural asphaltite as a solid disperse phase element on the structural and group composition of the residual heavy oil product and its malacometrical qualities (penetration, extensibility, softening point, resistance to aging and adhesion) is revealed. The production of samples of compounded bitumen production was carried out by introducing the required amount of the shredded asphaltite to deasphaltizat vacuum residue of heavy oil and heating their mixture to 220°С with vigorous stirring. Changes in the composition and physical and chemical properties of deasphalting the residual heavy oil product, associated with the amount of injected asphaltite, showed the possibility of production of modified bitumen with better adhesion properties that correspond to road bitumen

The composition of rocks and residual hydrocarbons in the flooded-out zones of romashkinskoye field reservoirs

Features of the change of the hydrocarbon composition of residual oil in the flooded-out zones of Pashlyskly Horizon reservoirs under long-term development are determined. Microstructural changes in rocks - reservoirs, caused by the degradation and regeneration processes, are revealed. It is shown, that asphaltenes and alcohol benzene resins dominated in the residual oil in strongly flooded-out sandy areas of terrigenous strata

The recurrences of cervical cancer: Possibilities of molecular prediction

The incidence of recurrence of cervical cancer ranges from 10 to 40 %. The 5-year survival rate for patients with recurrent cervical cancer is about 5–15 % against the background of current drug therapy. Clinical and morphological characteristics of the tumor process are known, which are used as markers of an unfavorable prognosis for the development of cervical cancer recurrence. The search for molecular prognostic markers of the course of cervical cancer continues.The aim. To determine the level of immune cycle proteins in patients with cervical cancer 0–IV stages, depending on the occurrence of a relapse of the disease.Materials and research methods. A retrospective analysis of previously obtained results of a study on the local level of immune cycle proteins in patients with cervical cancer was performed. Three years after follow-up, 2 groups were formed: group 1 – patients treated for cervical cancer without signs of disease progression (n = 83); group 2 – patients with cervical cancer with local or systemic recurrence (n = 18). Used statistical methods: non-parametric methods of statistics using the Kruskal – Wallis test; ROC-analysis for significant values in order to calculate threshold values; determination of the quality of the identified predictive markers by calculating the sensitivity, specificity, accuracy.Results. Local initial threshold values have a predictive value for predicting the occurrence of cervical cancer recurrence: B7.2 < 10.7 pg/ml (Se = 0.87; Sp = 0.73; Ac = 0.76; AUC = 0.78), PD-L1 ≤ 5.1 pg/ml (Se = 0.87; Sp = 0.68; Ac = 0.71; AUC = 0.76), sCD27 ≥ 32.0 pg/ml (Se = 0.75; Sp = 0.78; Ac = 0.78; AUC = 0.75).Conclusion. Determination of local levels of B7.2, PD-L1, sCD27 in patients with cervical cancer before treatment can be used to predict the development of disease recurrence during 3 years of follow-up

Transformation of residual oil in producing formations of the Romashkino oil field during hydrothermal treatment

Changes in the composition of organic matter of rocks and residual oil from multiage deposits of the Romashkino oil field in a reducing environment in a continuous-flow aqueous system were studied. It was shown that, as a result of the action of hydrothermal factors, the amount of hydrocarbon fractions increases and the amounts of alcohol-benzene-extractable resins, asphaltenes, and total sulfur decrease in organic extracts obtained from the rocks after hydrothermal experiments. The concentration of free radicals, as well as tetravalent vanadium (which is present in the form of vanadyl prophyrin complexes) and other heavy metals, in asphaltenes decreases. The hydrothermal treatment of petroliferous rock leads to the degradation of the structure of insoluble kerogen and unrecoverable components of residual oil, as well as to the washout of free hydrocarbons from the rock with the aqueous phase, n-Alkanes; ethyl, butyl, and octyl phthalates; furan; acids; and unsaturated oxygen-containing compounds with the isoprenoid structure were detected in the products of hydrothermal experiments isolated from aqueous extracts. © 2007 Pleiades Publishing, Ltd

Differentiation of Romashkino crude oils according to biomarker hydrocarbon parameters

The trends in composition of higher biomarker hydrocarbons (steranes and tri-, tetra-, and pentacyclic terpanes) in crude oils from diachronous producing complexes of deposits of the Romashkino oil field, which occurs in the central part of the South Tatarian arch, were studied. On the basis of variations in molecular biomarker parameters that reflect the type of source organic matter and the facies conditions of its accumulation and early diagenesis, the oils are classified with three groups. © Nauka/Interperiodica 2006