354 research outputs found
Cytokines and local chronic inflammation in the formation of infertility in fertile age women
The purpose of the study was to examine the characteristics of the content of cytokines in the peritoneal fluid in women of reproductive age with infertility against the background of chronic inflammatory diseases of the pelvic organs in the stage of clinical remission.Material and methods. A study was conducted of 50 patients aged 23-36 years with a verified diagnosis of infertility of tubal peritoneal genesis against a background of chronic inflammatory diseases of the reproductive sphere in the stage of clinical remission. As a control group, 15 practically healthy women aged 25-37 years were examined. Concentration of cytokines TNF-a, IL-4, IL-6, IL-10, IFN-y, MCP-1 in the peritoneal fluid was measured by enzyme-linked immunosorbent assay.Results and discussion. In the main group of patients with tubal-peritoneal infertility, the activity of local inflammatory reactions is tested in the period of clinical remission of a chronic inflammatory process. This is confirmed by a significantly higher level of TNF-a, IL-4, IL-6, IFN-y, MCP-1 in the peritoneal fluid compared to the control group. The studied biologically active substances play a significant role in the development of the immune response, as well as fibroplastic processes. The results can be regarded as the latent development of the immune response in reactions to constant antigenic stimulation from the focus of chronic inflammation, which is a significant factor in the development of adhesions in the formation of infertility, and requires the inclusion of immunotropic therapy methods in treatment
Comprehensive Treatment of Noninfectious Uveitis Accompanied by Macular Edema with the Use of Autologous Platelet-Rich Plasma
Background.Β A common cause of visual impairment in patients with non-infectious uveitis is macular edema, developing in 38β84 % of cases. Plasma enriched with platelets is widely used in various branches of medicine, the effectiveness of its use in the treatment of non-infectious uveitis, accompanied by macular edema, has not been sufficiently investigated.Β Aim:Β To evaluate the effectiveness of autologous platelet-rich plasma in the complex treatment of non-infectious uveitis accompanied by macular edema.Material and methods.Β The study was conducted on the basis of the academician S.N. Fyodorov Eye Microsurgery Federal State Institution in the period from 2016 to 2018, which included 123 people (176 eyes) from 18 to 50 years with non-infectious uveitis, accompanied by macular edema: 46 men, 77 women. Patients were divided into 2 groups. The main group consisted of patients receiving autologous platelet-rich plasma and anti-inflammatory treatment; the comparison group consisted of patients receiving anti-inflammatory treatment. The results of visual acuity, intraocular pressure, biomicroophthalmoscopy, optical coherence tomography of the macular zone, microperimetry, ultrasound examination on the side of the affected eye were evaluated. Statistical processing of the data was carried out in the program Statistica 10.Results.Β Maintenance of autologous platelet-rich plasma contributes to a statistically significant improvement in visual acuity on the 10thΒ day of treatment by 64.2 %, a decrease in the thickness of the retina in fovea by 36.3 % and an increase in retinal photosensitivity by 34.6 % compared to the group of patients receiving only anti-inflammatory treatment.Β Conclusions.Β The use of autologous platelet-rich plasma in the complex treatment of non-infectious uveitis allows to accelerate the natural mechanisms of tissue regeneration, contributing to the reduction of macular edema, and improve visual performance
ΠΠ»ΠΈΠ½ΠΈΠΊΠΎ-ΠΈΠΌΠΌΡΠ½ΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠ°Ρ Ρ Π°ΡΠ°ΠΊΡΠ΅ΡΠΈΡΡΠΈΠΊΠ° ΠΊΠ»ΠΈΠ½ΠΈΡΠ΅ΡΠΊΠΎΠΉ ΡΠ΅ΠΌΠΈΡΡΠΈΠΈ Ρ Π΄Π΅ΡΠ΅ΠΉ Ρ Ρ ΡΠΎΠ½ΠΈΡΠ΅ΡΠΊΠΈΠΌ ΠΏΠΈΠ΅Π»ΠΎΠ½Π΅ΡΡΠΈΡΠΎΠΌ ΠΏΠΎΡΠ»Π΅ Ρ ΠΈΡΡΡΠ³ΠΈΡΠ΅ΡΠΊΠΎΠΉ ΠΊΠΎΡΡΠ΅ΠΊΡΠΈΠΈ ΠΎΠ±ΡΡΡΡΠΊΡΠΈΠ²Π½ΡΡ ΡΡΠΎΠΏΠ°ΡΠΈΠΉ
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Clinical and diagnostic evaluation of the effectiveness of treatment of optic neuropathy in patients with edematous endocrine ophthalmopathy
The aim: to conduct a clinical and diagnostic evaluation of the effectiveness ofΒ theΒ administration of a complex of drugs in the region of hemolymphocirculation in optical neuropathy in patients with edematous form of endocrine ophthalmopathy.Materials and methods. The results of diagnosis and treatment of 31 patients (61 eyes) with optical neuropathy on the background of edematous exophthalmos in endocrine ophthalmopathy were analyzed. To identify hidden forms of optical neuropathy, such studies were prescribed as computer microperimetry on a confocal infrared ophthalmoscope, a complex of electroretinographic studies: registration of maximum ERG, oscillatory potentials. Latent forms of optical neuropathy were detected in 22 cases. In 9 cases, there were obvious forms of optical neuropathy. AllΒ patients underwent a 10-day course of intensive complex treatment, consisting of injections into the region of hemolymphocirculation (projection of the pterygoid fossa) No. 6β8 (No. 3β4 on each side) with an interval of 24 hours of a drug mixture, the formulation of which included Lidocaine 20 mg, Dexazone 4 mg, Hemase 3000 UNITS, Dalargin 1 mg.Results. In all patients, there was a pronounced positive dynamics in the form ofΒ aΒ significant (from 0.6 to 1.0) increase in visual acuity, a decrease in exophthalmos from (2.0 to 3.0 mm), an increase in color and contrast sensitivity.Conclusions. Due to the violation of venous and lymphatic outflow due to thickening of extraocular muscles and retrobulbar fiber, injections into the hemolymphocirculation region (projection of the pterygoid fossa) of drugs with a wide range of decongestant and metabolic effects are justified, effective and safe
Modern anatomical and physiological bases for maintaining the transparency of the corneal stroma
The article presents a literature review of the modern concept of anatomical and physiological structure and functioning of the cornea. The strict morphological structure and corneal tissue homeostasis ensure its transparency. Studying the mechanisms that regulate the constancy of the corneal tissue internal environment allows us to get closer to understanding the prospects forregenerative therapy for the corneal stroma pathology. The article discusses in detail the role and functional potential of corneal stromal cells, which are capable of reverse cytologic differentiation, which primarily ensures the maintenance of tissue homeostasis and corneal transparency. The functional activity of corneal cells can change for a number of reasons, which may be exogenous, iatrogenic (trauma, infection, etc.) or endogenous. Endogenous causes include: cell autoregulation pathologies (for example, enzyme defects); defects in transport systems leading to tissue hypoxia; disorders of the neuro-humoral regulation of trophism. The physical reason forthe violation of the corneal transparency is an increase in the light scattering. The article presents five main causes of increased light scattering in the opaque cornea, and also provides an overview of the main substances β components and products of cellular synthesis of corneal stromal cells: cytokines and growth factors (complex of the signal molecule and the SDF1/CXCR4 receptor, insulin-like growth factor 1, tumor necrosis factor alpha, intercellular adhesion molecule 1, erythropoietin, neurotrophic factors, etc.). Thus, corneal opacity can be caused by a single pathogenic mechanism or be the result of a complex effect of several factors. The main processes of tissue homeostasis regulation are aimed at maintaining the unique morphological structure of the cornea
Complex studies on gene polymorphisms of MMP2, MMP3, MMP9 matrix metalloproteinases and TIMP1, TIMP2 tissue inhibitors of metalloproteinases in the patients with primary open-angle glaucoma
Abnormal expression of matrix metalloproteinases (MMP) in watery moisture in patients with glaucoma may affect regulation of intraocular pressure (IOP). MMP activity is regulated by tissue metalloproteinase inhibitors (TIMP). The imbalance between tissue metalloproteinase inhibitors and matrix metalloproteinases may contribute to the development of glaucoma. Genetic factors, including polymorphism of matrix metalloproteinase genes and their inhibitors genes, can regulate the level of their expression, thereby affecting susceptibility to disease. Our aim was to perform comprehensive analysis of the MMP2 (rs243865), MMP3 (rs3025058), MMP9 (rs3918242) polymorphisms, and TIMP1 (rs4898), TIMP2 (rs8179090) tissue inhibitor genes polymorphisms in the patients with stage II (advanced) primary open-angle glaucoma.99 patients (52 men and 47 women) with a verified diagnosis of stage II primary open-angle glaucoma were examined. The comparison group consisted of 100 age-matched persons (81 women and 19 men) without ophthalmic disorders. The single-nucleotide polymorphisms in promoter regions of MMP2, TIMP1, TIMP2 genes were analyzed by the TaqMan method, the MMP3 and MMP9 genes, by means of restriction fragment length polymorphism technique. Statistical evaluation was carried out using the specialized package of IBM SPSS Statistics 23 programs. The critical level of significance was assumed to be 0.05.The differences in the distribution of MMP2 rs243865 allelotypes with decreased frequency of TT genotype were found in the patient group and, vice versa, increased heterozygosity rates were revealed among them. In addition, the frequency of TIMP1 rs4898 heterozygous genotype was decreased in this group as compared to control sample. Four MMP/TIMP complex genotypes are positively associated with the development of pathology. Two of them were of bilocus type, i.e., MMP2-1306TC:TIMP2-418GG, and MMP3-11715A6A:TIMP1 372CC whereas two three-locus constellations were revealed, i.e., MMP2-1306TC:MMP9-1562CC:TIMP2- 418GG, and MMP3-11715A6A:MMP9-1562CC:TIMP1 372CC. There are nine MMP/TIMP complexes, the frequency of which in patients with glaucoma was significantly reduced when compared with control group.Polymorphism of regulatory regions of MMP2, MMP3, MMP9 genes and distinct gene variants of their inhibitors (TIMP1, TIMP2 genes) can be considered potential markers of the POAG development associated with an imbalance of MMP/TIMP activities
THE ELEMENTS OF INTRAOCULAR FLUID LYMPHATIC OUTFLOW PATHWAYS IN CHOROID IN NORM AND IN GLAUCOMA PATIENTS
Purpose. To detect and study the structure of aqueous humor lymphatic outflow pathways in choroid in normotensive patients and patients with primary open-angle glaucoma (POAG).Material and methods. Choroid fragments of seven human eyes (including two eyes with terminal glaucoma) enucleated on medical indications were studied. The structure of the choroid was investigated using immunohistochemistry and electron microscopy.Results. Lymphatic channels and lymphatic lacunae were revealed in the choroid structure. Lymphatic channels were detected in choriocapillar and vascular layers and were limited by Podoplanin+, Prox-1+, LYVE-1+ endothelium-like cells, fibroblasts and pigment cells. Lymphatic lacunae were located in suprachoroid layer and covered with endothelium-like cells and fibroblasts. Morphometric study showed an increase of the volume density of epithelium, interstitial spaces and choroid vessels related to edema and swelling of the choroidal stroma in the terminal stage of glaucoma.Conclusions. Human choroid contains lymphatic structures that are probably a part of the ocular lymphatic drainage system and participate in the aqueous humor outflow. Choroidal edema and swelling, the increase of the volume density of epithelium, interstitial spaces and choroid vessels in the terminal POAG stage indicate the lymphatic drainage dysfunction beginning from initial stages of glaucoma and lead to an aqueous humor outflow alteration through the protective lymphatic system
ΠΠ»ΠΈΠ½ΠΈΠΊΠΎ-ΠΈΠΌΠΌΡΠ½ΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈΠ΅ ΠΌΠ°ΡΠΊΠ΅ΡΡ Π³Π΅Π½Π΅ΡΠ°Π»ΠΈΠ·ΠΎΠ²Π°Π½Π½ΡΡ ΠΈΠ½ΡΠ΅ΠΊΡΠΈΠΉ Ρ Π½ΠΎΠ²ΠΎΡΠΎΠΆΠ΄Π΅Π½Π½ΡΡ Π² Π·Π°Π²ΠΈΡΠΈΠΌΠΎΡΡΠΈ ΠΎΡ ΡΡΠ΅ΠΏΠ΅Π½ΠΈ Π·ΡΠ΅Π»ΠΎΡΡΠΈ ΠΎΡΠ³Π°Π½ΠΈΠ·ΠΌΠ°
.ΠΡΠΈ Π²ΡΠΎΠΆΠ΄Π΅Π½Π½ΡΡ
Π³Π΅Π½Π΅ΡΠ°Π»ΠΈΠ·ΠΎΠ²Π°Π½Π½ΠΎΠ³ΠΎ ΡΠΈΠΏΠ° ΠΠΠ-Π²ΠΈΡΡΡΠ½ΡΡ
ΠΈΠ½ΡΠ΅ΠΊΡΠΈΡΡ
ΠΊΠ°ΠΊ Ρ Π΄ΠΎΠ½ΠΎΡΠ΅Π½Π½ΡΡ
, ΡΠ°ΠΊ ΠΈ Π½Π΅Π΄ΠΎΠ½ΠΎΡΠ΅Π½Π½ΡΡ
Π½ΠΎΠ²ΠΎΡΠΎΠΆΠ΄Π΅Π½Π½ΡΡ
ΡΠ΅Π³ΠΈΡΡΡΠΈΡΡΠ΅ΡΡΡ ΠΏΠΎΠ»ΠΈΠΎΡΠ³Π°Π½Π½ΡΠΉ ΡΠΈΡΡΠ΅ΠΌΠ½ΡΠΉ Ρ
Π°ΡΠ°ΠΊΡΠ΅Ρ ΠΏΠΎΡΠ°ΠΆΠ΅Π½ΠΈΡ Π² Π²ΠΈΠ΄Π΅ ΡΠ΅ΡΠ°Π»ΡΠ½ΠΎΠ³ΠΎ Π³Π΅ΠΏΠ°ΡΠΈΡΠ° Ρ ΡΠ²Π»Π΅Π½ΠΈΡΠΌΠΈ Ρ
ΠΎΠ»Π΅ΡΡΠ°Π·Π°, ΠΌΠ΅Π½ΠΈΠ½Π³ΠΎΡΠ½ΡΠ΅ΡΠ°Π»ΠΈΡΠ°, ΡΡΠ±ΡΠ»ΠΎΠΈΠ½ΡΠ΅ΡΡΡΠΈΡΠΈΠ°Π»ΡΠ½ΠΎΠ³ΠΎ Π½Π΅ΡΡΠΈΡΠ°, ΠΏΠ½Π΅Π²ΠΌΠΎΠ½ΠΈΠΈ. ΠΠ°ΡΡΠ΄Ρ Ρ ΡΡΠΈΠΌ ΠΎΡ 30 Π΄ΠΎ 50% Π΄Π΅ΡΠ΅ΠΉ ΠΈΠΌΠ΅ΡΡ ΠΏΠΎΡΠ°ΠΆΠ΅Π½ΠΈΡ ΠΆΠ΅Π»ΡΠ΄ΠΎΡΠ½ΠΎ-ΠΊΠΈΡΠ΅ΡΠ½ΠΎΠ³ΠΎ ΡΡΠ°ΠΊΡΠ° Π² ΡΠΎΡΠΌΠ΅ Π―ΠΠΠ, Π³Π΅ΠΌΠΎΡΡΠ°Π³ΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΡΠΈΠ½Π΄ΡΠΎΠΌΠ°, Π΄ΠΎ 30% ΠΏΠ°ΡΠΈΠ΅Π½ΡΠΎΠ² ΡΠΎΡΠΌΠΈΡΡΡΡ ΡΠΈΠ½Π΄ΡΠΎΠΌ ΠΏΠΎΠ»ΠΈΠΎΡΠ³Π°Π½Π½ΠΎΠΉ Π½Π΅Π΄ΠΎΡΡΠ°ΡΠΎΡΠ½ΠΎΡΡΠΈ. Π ΠΏΠΎΠΊΠ°Π·Π°ΡΠ΅Π»ΡΡ
Π³Π΅ΠΌΠΎΠ³ΡΠ°ΠΌΠΌΡ ΠΏΡΠΈ Π³Π΅Π½Π΅ΡΠ°Π»ΠΈΠ·ΠΎΠ²Π°Π½Β¬Π½ΡΡ
Π²ΠΈΡΡΡΠ½ΡΡ
ΠΈΠ½ΡΠ΅ΠΊΡΠΈΡΡ
ΠΈΠ·ΠΌΠ΅Π½Π΅Π½ΠΈΡ ΠΊΠ°ΡΠ°ΡΡΡΡ Π²ΠΎ Π²ΡΠ΅Ρ
ΡΠ»ΡΡΠ°ΡΡ
ΡΡΠΈΡΡΠΎΠΈΠ΄Π½ΠΎΠ³ΠΎ ΠΈ ΡΡΠΎΠΌΠ±ΠΎΡΠΈΡΠ°ΡΠ½ΠΎΠ³ΠΎ ΡΠΎΡΡΠΊΠΎΠ², Ρ
Π°ΡΠ°ΠΊΡΠ΅ΡΠΈΠ·ΡΡΡΡ ΠΏΡΠΎΠ³ΡΠ΅ΡΡΠΈΡΡΡΡΠ΅ΠΉ Π°Π½Π΅ΠΌΠΈΠ΅ΠΉ ΠΈ ΡΡΠΎΠΌΠ±ΠΎΡΠΈΡΠΎΠΏΠ΅Π½ΠΈΠ΅ΠΉ. ΠΠΌΠ΅ΡΡΠ΅ Ρ ΡΠ΅ΠΌ ΠΈΠ·ΠΌΠ΅Π½Π΅Π½ΠΈΡ ΡΡΠΎΠ²Π½Ρ Π»Π΅ΠΉΠΊΠΎΡΠΈΡΠΎΠ² Π½Π΅ Π½ΠΎΡΡΡ ΠΏΠΎΡΡΠΎΡΠ½Π½ΠΎΠ³ΠΎ Ρ
Π°ΡΠ°ΠΊΡΠ΅ΡΠ°, Π½Π΅ ΠΎΡΠΊΠ»ΠΎΠ½ΡΡΡΡ ΠΎΡ Π½ΠΎΡΠΌΠ°ΡΠΈΠ²Π½ΡΡ
ΠΏΠΎΠΊΠ°Π·Π°ΡΠ΅Π»Π΅ΠΉ Π»ΠΈΠ±ΠΎ ΡΠΎΡΠΌΠΈΡΡΡ Π½Π΅ΠΉΡΡΠΎ- ΠΈ Π»Π΅ΠΉΠΊΠΎΠΏΠ΅Π½ΠΈΡ. Π£ΡΠΎΠ²Π½ΠΈ ΠΏΡΠΎ- ΠΈ ΠΏΡΠΎΡΠΈΠ²ΠΎΡΠΏΠ°Π»ΠΈΡΠ΅Π»ΡΠ½ΡΡ
ΡΠΈΡΠΎΠΊΠΈΠ½ΠΎΠ² ΠΏΠΎΠ²ΡΡΠ°ΡΡΡΡ Ρ Π²ΡΠ΅Ρ
ΠΏΠ°ΡΠΈΠ΅Π½ΡΠΎΠ² Ρ Π³Π΅Π½Π΅ΡΠ°Π»ΠΈΠ·ΠΎΠ²Π°Π½Π½ΡΠΌΠΈ Π²ΠΈΡΡΡΠ½ΡΠΌΠΈ ΠΈΠ½ΡΠ΅ΠΊΡΠΈΡΠΌΠΈ, ΠΏΠΎ ΠΎΠΏΡΠ΅Π΄Π΅Π»Π΅Π½Π½ΡΠΌ ΠΏΠΎΠΊΠ°Π·Π°ΡΠ΅Π»ΡΠΌ ΡΡΠΎΠ²Π΅Π½Ρ ΠΏΡΠΎΠ²ΠΎΡΠΏΠ°Π»ΠΈΡΠ΅Π»ΡΠ½ΡΡ
ΡΠΈΡΠΎΠΊΠΈΠ½ΠΎΠ² Ρ Π΄ΠΎΠ½ΠΎΡΠ΅Π½Π½ΡΡ
Π½ΠΎΠ²ΠΎΡΠΎΠΆΠ΄Π΅Π½Π½ΡΡ
Π΄ΠΎΡΡΠΎΠ²Π΅ΡΠ½ΠΎ Π²ΡΡΠ΅, ΡΠ΅ΠΌ Ρ Π½Π΅Π΄ΠΎΠ½ΠΎΡΠ΅Π½Π½ΡΡ
. ΠΠ°ΡΡΠ΄Ρ Ρ ΡΡΠΈΠΌ ΠΊΠ°ΠΊ Ρ Π½Π΅Π΄ΠΎΠ½ΠΎΡΠ΅Π½Π½ΡΡ
Π΄Π΅ΡΠ΅ΠΉ, ΡΠ°ΠΊ ΠΈ Π΄ΠΎΠ½ΠΎΡΠ΅Π½Π½ΡΡ
Π½ΠΎΠ²ΠΎΡΠΎΠΆΠ΄Π΅Π½Π½ΡΡ
ΠΎΡΠΌΠ΅ΡΠ΅Π½Ρ Π΄ΠΎΡΡΠΎΠ²Π΅ΡΠ½ΠΎ Π±ΠΎΠ»Π΅Π΅ Π²ΡΡΠΎΠΊΠΈΠ΅ ΠΏΠΎΠΊΠ°Π·Π°ΡΠ΅Π»ΠΈ ΠΠ€Π-Ρ, ΡΠ΅ΠΌ ΠΠ-1Ξ², ΠΎΠ΄Π½Π°ΠΊΠΎ Ρ Π½Π΅Π΄ΠΎΠ½ΠΎΡΠ΅Π½Π½ΡΡ
Π΄Π΅ΡΠ΅ΠΉ Π΅Π³ΠΎ ΡΡΠΎΠ²Π΅Π½Ρ ΡΠ°ΠΊΠΆΠ΅ Π±ΡΠ» Π΄ΠΎΡΡΠΎΠ²Π΅ΡΠ½ΠΎ Π½ΠΈΠΆΠ΅, ΡΠ΅ΠΌ Ρ Π΄ΠΎΠ½ΠΎΡΠ΅Π½Π½ΡΡ
. Π’Π°ΠΊΠΈΠΌ ΠΎΠ±ΡΠ°Π·ΠΎΠΌ, ΠΏΠΎΠ»ΡΡΠ΅Π½Π½ΡΠ΅ Π΄Π°Π½Π½ΡΠ΅ ΡΠ²ΠΈΠ΄Π΅ΡΠ΅Π»ΡΡΡΠ²ΡΡΡ ΠΎ ΡΠΎΠΌ, ΡΡΠΎ Ρ
Π°ΡΠ°ΠΊΡΠ΅Ρ ΡΠ΅ΡΠ΅Π½ΠΈΡ ΠΈΠ½ΡΠ΅ΠΊΡΠΈΠΎΠ½Π½ΠΎΠ³ΠΎ ΠΏΡΠΎΡΠ΅ΡΡΠ° ΠΏΡΠΈ ΠΠΠ-Π²ΠΈΡΡΡΠ½ΡΡ
ΠΈΠ½ΡΠ΅ΠΊΡΠΈΡΡ
Ρ Π½ΠΎΠ²ΠΎΡΠΎΠΆΠ΄Π΅Π½Π½ΡΡ
ΡΠ²Π»ΡΠ΅ΡΡΡ Π³Π΅Π½Π΅ΡΠ°Π»ΠΈΠ·ΠΎΠ²Π°Π½Π½ΡΠΌ, ΡΡΠΎ ΠΎΠΏΡΠ΅Π΄Π΅Π»ΡΠ΅ΡΡΡ ΠΊΠ°ΠΊ ΡΠ°Π·ΠΎΠΉ ΡΠ΅ΡΠ΅Π½ΠΈΡ ΠΈΠ½ΡΠ΅ΠΊΡΠΈΠΎΠ½Π½ΠΎΠ³ΠΎ ΠΏΡΠΎΡΠ΅ΡΡΠ°, ΠΈΠΌΠΌΡΠ½ΠΎ-ΠΊΠΎΠΌΠΏΠ΅ΡΠ΅Π½ΡΠ½ΠΎΡΡΡΡ ΠΎΡΠ³Π°Π½ΠΈΠ·ΠΌΠ°, Π·Π°Π²ΠΈΡΡΡΠ΅ΠΉ ΠΎΡ ΡΡΠ΅ΠΏΠ΅Π½ΠΈ Π·ΡΠ΅Π»ΠΎΡΡΠΈ ΠΎΡΠ³Π°Π½ΠΈΠ·ΠΌΠ°, ΡΠ°ΠΊ ΠΈ ΡΠ²ΠΎΠΉΡΡΠ²Π°ΠΌΠΈ Π²ΠΎΠ·Π±ΡΠ΄ΠΈΡΠ΅Π»Π΅ΠΉ, ΠΊΠΎΡΠΎΡΡΠ΅ Π² Ρ
ΠΎΠ΄Π΅ ΡΠ²ΠΎΠ»ΡΡΠΈΠΈ ΠΏΡΠΈΠΎΠ±ΡΠ΅Π»ΠΈ ΡΠΏΠΎΡΠΎΠ±Π½ΠΎΡΡΡ ΡΡΠΊΠΎΠ»ΡΠ·Π°ΡΡ ΠΎΡ ΡΠΈΡΡΠ΅ΠΌΡ ΠΈΠΌΠΌΡΠ½Π½ΠΎΠ³ΠΎ Π½Π°Π΄Π·ΠΎΡΠ° ΠΈ ΡΠΏΠΎΡΠΎΠ±Π½ΠΎΡΡΡ Π·Π°Π²ΡΠ°Π»ΠΈΡΠΎΠ²Π°ΡΡΡΡ, ΠΎΠΏΡΠ΅Π΄Π΅Π»ΡΡ ΡΠ΅ΡΠΈΠ΄ΠΈΠ²ΠΈΡΡΡΡΠ΅Π΅ Π»ΠΈΠ±ΠΎ ΠΏΠ΅ΡΡΠΈΡΡΠΈΡΡΡΡΠ΅Π΅ ΡΠ΅ΡΠ΅Π½ΠΈΠ΅ Π²ΡΠΎΠΆΠ΄Π΅Π½Π½ΡΡ
Π²ΠΈΡΡΡΠ½ΡΡ
ΠΈΠ½ΡΠ΅ΠΊΡΠΈΠΉ
POLYMORPHISMS OF EXTRACELLULAR CONNECTIVE TISSUE REMODELING PROTEINASES AND <i>MMP2, MMP3, MMP9</i> GENES, AND NEOANGIGENESIS <i>VEGF</i> GENE IN RETINAL MICROANGIOPATHY IN THE PATIENTS WITH TYPE 2 DIABETES MELLITUS
The aim of our study was to perform an association analysis between MMP2, MMP3, MMP9, VEGF gene polymorphisms and development of non-proliferative diabetic retinopathy (DR) in the type 2 diabetic patients (DM).201 DM patients: 90 cases of DR and 111 subjects without DR features were included into the study. Polymorphic variants of MMP2 (rs2438650), MMP3 (rs3025058), MMP9 (rs3918242), and VEGF (rs699947 and rs3025039) genes were assayed. The genetic typing was carried out by restriction fragment length polymorphism and TaqMan methods.The analysis of complex genotypes at the five polymorphic positions has revealed some significant findings in positive and negatively incorporated complexes. Increased frequencies of MMP2-1306 CC genotype in the group of patients with βearlyβ development of complication, and more frequent combination of high-level HbA1c with MMP2-1306CC and MMP9-1562CT genotypes were shown in DR patients. Computerassisted modelling with visual reconstruction of network interactions between the genotypes involved into the destruction events and angiogenesis, as well as altered HbA1Ρ levels (an integral parameter of glycemia), has revealed some differences in structural and functional organization of gene-gene and gene- protein interactions between the groups of patients with DR versus those without this disorder. Π‘onclusion. A design of interactome biological networks based on transcription regulation and metabolic pathways, as well as their topological analysis allows to build and study interactions of genes and proteins, with reference to pathogenetic studies of DM2 complications aiming for development of approaches to personalized prevention and therapy in future times
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