DNA methylation-based estimator of telomere length

Telomere length (TL) is associated with several aging-related diseases. Here, we present a DNA methylation estimator of TL (DNAmTL) based on 140 CpGs. Leukocyte DNAmTL is applicable across the entire age spectrum and is more strongly associated with age than measured leukocyte TL (LTL) (r ~-0.75 for DNAmTL versus r ~ -0.35 for LTL). Leukocyte DNAmTL outperforms LTL in predicting: i) time-to-death (p=2.5E-20), ii) time-to-coronary heart disease (p=6.6E-5), iii) time-to-congestive heart failure (p=3.5E-6), and iv) association with smoking history (p=1.21E-17). These associations are further validated in large scale methylation data (n=10k samples) from the Framingham Heart Study, Women's Health Initiative, Jackson Heart Study, InChianti, Lothian Birth Cohorts, Twins UK, and Bogalusa Heart Study. Leukocyte DNAmTL is also associated with measures of physical fitness/functioning (p=0.029), age-at-menopause (p=0.039), dietary variables (omega 3, fish, vegetable intake), educational attainment (p=3.3E-8) and income (p=3.1E-5). Experiments in cultured somatic cells show that DNAmTL dynamics reflect in part cell replication rather than TL per se. DNAmTL is not only an epigenetic biomarker of replicative history of cells, but a useful marker of age-related pathologies that are associated with it

Thermochrom beschichtete Substrate und Verfahren zu deren Herstellung

WO 2009106352 A1 UPAB: 20090923 NOVELTY - Process for thermochromic coating of a substrate comprises contacting the substrate and at least an organic thermochrome precursor containing at least a dye, at least a developer and at least a flux into a vacuum chamber; evaporating the precursor and depositing on substrate as a thermochromic complex. DETAILED DESCRIPTION - An INDEPENDENT CLAIM is included for the thermochrome coated substrate obtained by the above process. USE - The thermochrome coated substrate is useful to manufacture electro-optical or photovoltaic modules (claimed). ADVANTAGE - The process provides thermochrome coated substrate with a freely selectable temperature shift between different color states and a multi-switch possible between the color states. The process is easy to handle and provides high reproducibility. The thermochromic materials used in the process: exhibits high light stability, and transparency, and is nontoxic