35 research outputs found
Quantitative Imaging of Protein-Protein Interactions by Multiphoton Fluorescence Lifetime Imaging Microscopy using a Streak camera
Full text linkFluorescence Lifetime Imaging Microscopy (FLIM) using multiphoton excitation
techniques is now finding an important place in quantitative imaging of
protein-protein interactions and intracellular physiology. We review here the
recent developments in multiphoton FLIM methods and also present a description
of a novel multiphoton FLIM system using a streak camera that was developed in
our laboratory. We provide an example of a typical application of the system in
which we measure the fluorescence resonance energy transfer between a
donor/acceptor pair of fluorescent proteins within a cellular specimen.Comment: Overview of FLIM techniques, StreakFLIM instrument, FRET application
Proinsulin Associates with Glycaemic Control and Insulin Dose Independent of Stimulated C-Peptide in Children with New Onset Type 1 Diabetes. Results from the Hvidoere Study Group
No full textMid-sagittal cut to area function transformations: Direct measurements of mid-sagittal distance and area with MRI
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Electromagnetic Articulography Treatment for an Adult With Broca’s Aphasia and Apraxia of Speech
No full textDiffusion Bias and Photophysical Dynamics of Single Molecules in Unsupported Lipid Bilayer Membranes Probed with Confocal Microscopy
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