13,948 research outputs found
Metabolic Impacts of Using Nitrogen and Copper-Regulated Promoters to Regulate Gene Expression in Neurospora crassa.
The filamentous fungus Neurospora crassa is a long-studied eukaryotic microbial system amenable to heterologous expression of native and foreign proteins. However, relatively few highly tunable promoters have been developed for this species. In this study, we compare the tcu-1 and nit-6 promoters for controlled expression of a GFP reporter gene in N. crassa. Although the copper-regulated tcu-1 has been previously characterized, this is the first investigation exploring nitrogen-controlled nit-6 for expression of heterologous genes in N. crassa. We determined that fragments corresponding to 1.5-kb fragments upstream of the tcu-1 and nit-6 open reading frames are needed for optimal repression and expression of GFP mRNA and protein. nit-6 was repressed using concentrations of glutamine from 2 to 20 mM and induced in medium containing 0.5-20 mM nitrate as the nitrogen source. Highest levels of expression were achieved within 3 hr of induction for each promoter and GFP mRNA could not be detected within 1 hr after transfer to repressing conditions using the nit-6 promoter. We also performed metabolic profiling experiments using proton NMR to identify changes in metabolite levels under inducing and repressing conditions for each promoter. The results demonstrate that conditions used to regulate tcu-1 do not significantly change the primary metabolome and that the differences between inducing and repressing conditions for nit-6 can be accounted for by growth under nitrate or glutamine as a nitrogen source. Our findings demonstrate that nit-6 is a tunable promoter that joins tcu-1 as a choice for regulation of gene expression in N. crassa
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The SNARE protein FolVam7 mediates intracellular trafficking to regulate conidiogenesis and pathogenicity in Fusarium oxysporum f. sp. lycopersici.
Soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNAREs) are conserved in fungi, plants and animals. The Vam7 gene encodes a v-SNARE protein that involved in vesicle trafficking in fungi. Here, we identified and characterized the function of FolVam7, a homologue of the yeast SNARE protein Vam7p in Fusarium oxysporum f. sp. lycopersici (Fol), a fungal pathogen of tomato. FolVam7 contains SNARE and PX (Phox homology) domains that are indispensable for normal localization and function of FolVam7. Targeted gene deletion showed that FolVam7-mediated vesicle trafficking is important for vegetative growth, asexual development, conidial morphology and plant infection. Further cytological examinations revealed that FolVam7 is localized to vesicles and vacuole membranes in the hyphae stage. Moreover, the ΔFolvam7 mutant is insensitive to salt and osmotic stresses and hypersensitive to cell wall stressors. Taken together, our results suggested that FolVam7-mediated vesicle trafficking promotes vegetative growth, conidiogenesis and pathogenicity of Fol
Monte Carlo simulation of melting transition on DNA nanocompartment
DNA nanocompartment is a typical DNA-based machine whose function is
dependent of molecular collective effect. Fundamental properties of the device
have been addressed via electrochemical analysis, fluorescent microscopy, and
atomic force microscopy. Interesting and novel phenomena emerged during the
switching of the device. We have found that DNAs in this system exhibit a much
steep melting transition compared to ones in bulk solution or conventional DNA
array. To achieve an understanding to this discrepancy, we introduced DNA-DNA
interaction potential to the conventional Ising-like Zimm-Bragg theory and
Peyrard-Bishop model of DNA melting. To avoid unrealistic numerical calculation
caused by modification of the Peyrard-Bishop nonlinear Hamiltonian with the
DNA-DNA interaction, we established coarse-gained Monte Carlo recursion
relations by elucidation of five components of energy change during melting
transition. The result suggests that DNA-DNA interaction potential accounts for
the observed steep transition.Comment: 12 pages, 5 figure
A somatic mutation in moesin drives progression into acute myeloid leukemia
Acute myeloid leukemia (AML) arises when leukemia-initiating cells, defined by a primary genetic lesion, acquire subsequent molecular changes whose cumulative effects bypass tumor suppression. The changes that underlie AML pathogenesis not only provide insights into the biology of transformation but also reveal novel therapeutic opportunities. However, backtracking these events in transformed human AML samples is challenging, if at all possible. Here, we approached this question using a murine in vivo model with an MLL-ENL fusion protein as a primary molecular event. Upon clonal transformation, we identified and extensively verified a recurrent codon-changing mutation (Ar
Existence and Stability of Symmetric Periodic Simultaneous Binary Collision Orbits in the Planar Pairwise Symmetric Four-Body Problem
We extend our previous analytic existence of a symmetric periodic
simultaneous binary collision orbit in a regularized fully symmetric equal mass
four-body problem to the analytic existence of a symmetric periodic
simultaneous binary collision orbit in a regularized planar pairwise symmetric
equal mass four-body problem. We then use a continuation method to numerically
find symmetric periodic simultaneous binary collision orbits in a regularized
planar pairwise symmetric 1, m, 1, m four-body problem for between 0 and 1.
Numerical estimates of the the characteristic multipliers show that these
periodic orbits are linearly stability when , and are
linearly unstable when .Comment: 6 figure
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