Living benthic foraminifera around the unique Umm al Maradim Island (Kuwait)

The present investigation focuses on the unique Island of Umm al Maradim that represents the largest and southernmost coral island in Kuwait. Thirty-seven stations around the Island were sampled for living benthic foraminiferal analyses. Relatively high values of diversity for both total and living assemblages, 101 and 96 species, respectively, have been documented. These figures are sensibly higher than those documented in most of the previous studies around Kuwait territorial waters. Porcelaneous test in terms of abundance dominates the foraminiferal assemblages followed by hyaline tests and only a minor part of the assemblages was represented by agglutinated foraminifera. The high dominance of porcelaneous tests might be explained by the peculiar physiographic setting and the presence of coral reef around the Island. Very low foraminiferal abundances were associated with shallower environments, like the subtidal zone around the island, whereas higher values were found at deeper and more distal stations. Planispiral morphotypes were found dominant at the more shallow-proximal stations, while milioline morphotypes dominated at intermediate depth and trochospiral morphotypes were more abundant at greater water depth. This paper contributes towards the documentation of benthic foraminiferal diversity around the islands in the Kuwait territorial water

Short-Term Autophagy Preconditioning Upregulates the Expression of COX2 and PGE2 and Alters the Immune Phenotype of Human Adipose-Derived Stem Cells In Vitro

Human adipose-derived stem cells (hASCs) are potent modulators of inflammation and promising candidates for the treatment of inflammatory and autoimmune diseases. Strategies to improve hASC survival and immunoregulation are active areas of investigation. Autophagy, a homeostatic and stress-induced degradative pathway, plays a crucial role in hASC paracrine signaling—a primary mechanism of therapeutic action. Therefore, induction of autophagy with rapamycin (Rapa), or inhibition with 3-methyladenine (3-MA), was examined as a preconditioning strategy to enhance therapeutic efficacy. Following preconditioning, both Rapa and 3-MA-treated hASCs demonstrated preservation of stemness, as well as upregulated transcription of cyclooxygenase-2 (COX2) and interleukin-6 (IL-6). Rapa-ASCs further upregulated TNFα-stimulated gene-6 (TSG-6) and interleukin-1 beta (IL-1β), indicating additional enhancement of immunomodulatory potential. Preconditioned cells were then stimulated with the inflammatory cytokine interferon-gamma (IFNγ) and assessed for immunomodulatory factor production. Rapa-pretreated cells, but not 3-MA-pretreated cells, further amplified COX2 and IL-6 transcripts following IFNγ exposure, and both groups upregulated secretion of prostaglandin-E2 (PGE2), the enzymatic product of COX2. These findings suggest that a 4-h Rapa preconditioning strategy may bestow the greatest improvement to hASC expression of cytokines known to promote tissue repair and regeneration and may hold promise for augmenting the therapeutic potential of hASCs for inflammation-driven pathological conditions

Short-Term Rapamycin Preconditioning Diminishes Therapeutic Efficacy of Human Adipose-Derived Stem Cells in a Murine Model of Multiple Sclerosis

Human adipose-derived stem cells (ASCs) show immense promise for treating inflammatory diseases, attributed primarily to their potent paracrine signaling. Previous investigations demonstrated that short-term Rapamycin preconditioning of bone marrow-derived stem cells (BMSCs) elevated secretion of prostaglandin E2, a pleiotropic molecule with therapeutic effects in the experimental autoimmune encephalomyelitis (EAE) model of multiple sclerosis (MS), and enhanced immunosuppressive capacity in vitro. However, this has yet to be examined in ASCs. The present study examined the therapeutic potential of short-term Rapamycin-preconditioned ASCs in the EAE model. Animals were treated at peak disease with control ASCs (EAE-ASCs), Rapa-preconditioned ASCs (EAE-Rapa-ASCs), or vehicle control (EAE). Results show that EAE-ASCs improved clinical disease scores and elevated intact myelin compared to both EAE and EAE-Rapa-ASC animals. These results correlated with augmented CD4+ T helper (Th) and T regulatory (Treg) cell populations in the spinal cord, and increased gene expression of interleukin-10 (IL-10), an anti-inflammatory cytokine. Conversely, EAE-Rapa-ASC mice showed no improvement in clinical disease scores, reduced myelin levels, and significantly less Th and Treg cells in the spinal cord. These findings suggest that short-term Rapamycin preconditioning reduces the therapeutic efficacy of ASCs when applied to late-stage EAE

Short-Term Rapamycin Preconditioning Diminishes Therapeutic Efficacy of Human Adipose-Derived Stem Cells in a Murine Model of Multiple Sclerosis.

Human adipose-derived stem cells (ASCs) show immense promise for treating inflammatory diseases, attributed primarily to their potent paracrine signaling. Previous investigations demonstrated that short-term Rapamycin preconditioning of bone marrow-derived stem cells (BMSCs) elevated secretion of prostaglandin E2, a pleiotropic molecule with therapeutic effects in the experimental autoimmune encephalomyelitis (EAE) model of multiple sclerosis (MS), and enhanced immunosuppressive capacity in vitro. However, this has yet to be examined in ASCs. The present study examined the therapeutic potential of short-term Rapamycin-preconditioned ASCs in the EAE model. Animals were treated at peak disease with control ASCs (EAE-ASCs), Rapa-preconditioned ASCs (EAE-Rapa-ASCs), or vehicle control (EAE). Results show that EAE-ASCs improved clinical disease scores and elevated intact myelin compared to both EAE and EAE-Rapa-ASC animals. These results correlated with augmented CD

Environmental rejuvenation of the Gulf by compensation and restoration

The Gulf is considered to be a young sea in decline, with poor prognosis for continuing production of abundant natural resources. We compare and contrast ‘monetary’ and ‘environmental’ compensation as mechanisms for addressing ecosystem damage in the Gulf. The 1992 International Oil Pollution Compensation Conventions settle claims financially, but only for certain categories of oil spills. For example, aside from inherent difficulties of valuing ecosystem services and their losses, ecological damage from the time of injury to recovery (interim losses) is not compensated. Another approach involves reimbursement for environmental action/projects to restore affected resources and offset impacts until recovery. In habitat equivalency analysis, mitigation requirements are calculated from the type(s), severity, duration and extent of resource impacts. This approach was utilized to resolve several claims for damage from the 1991 Gulf War oil spill. Various compensatory projects resulted, including direct oil spill remediation and other environmental projects such as the establishment of ≥1 protected area (x ha for y years). Besides compensation, in this paper we advocate setting threshold levels for the protection of different coastal and marine ecosystems. This could be achieved by a proportion (c. 30%) of every major ecosystem becoming fully protected, through an expanded regional protected area network