1,461 research outputs found
Consumer attitudes and preference exploration towards fresh-cut salads using best–worst scaling and latent class analysis
This research explored the preferences and buying habits of a sample of 620 consumers of fresh-cut, ready-to-eat salads. A best–worst scaling approach was used to measure the level of preference stated by individuals regarding 12 attributes for quality (intrinsic, extrinsic and credence) of fresh-cut salads. The experiment was carried out through direct interviews at several large-scale retail outlets in the Turin metropolitan area (north-west of Italy). Out of the total number of questioned consumers, 35% said they did not consume fresh-cut salads. On the contrary, the rest of the involved sample expressed the highest degree of preference towards the freshness/appearance attribute, followed by the expiration date and the brand. On the contrary, attributes such as price, organic certification and food safety did not emerge as discriminating factors in consumer choices. Additionally, five clusters of consumers were identified, whose preferences are related both to purchasing styles and socio-demographic variables. In conclusion, this research has highlighted the positive attitude of consumers towards quality products backed by a brand, providing ideas for companies to improve within this sector and implement strategies to answer the needs of a new segment of consumers, by determining market opportunities that aim to strengthen local brands
How to change the oligomeric state of a circular protein assembly: switch from 11-subunit to 12-subunit TRAP suggests a general mechanism
Many critical cellular functions are performed by multisubunit circular protein oligomers whose internal geometry has evolved to meet functional requirements. The subunit number is arguably the most critical parameter of a circular protein assembly, affecting the internal and external diameters of the assembly and often impacting on the protein's function. Although accurate structural information has been obtained for several circular proteins, a lack of accurate information on alternative oligomeric states has prevented engineering such transitions. In this study we used the bacterial transcription regulator TRAP as a model system to investigate the features that define the oligomeric state of a circular protein and to question how the subunit number could be manipulated.We find that while Bacillus subtilis and Bacillus stearothermophilus TRAP form 11-subunit oligomers, the Bacillus halodurans TRAP exclusively forms 12-subunit assemblies. Significantly, the two states of TRAP are related by a simple rigid body rotation of individual subunits around inter-subunit axes. We tested if such a rotation could be induced by insertion or deletion mutations at the subunit interface. Using wild type 11-subunit TRAP, we demonstrate that removal of five C-terminal residues at the outer side of the inter-subunit axis or extension of an amino acid side chain at the opposite, inner side, increased the subunit number from 11 to 12. Our findings are supported by crystal structures of TRAP oligomers and by native mass spectrometry data.The subunit number of the TRAP oligomer can be manipulated by introducing deletion or addition mutations at the subunit interface. An analysis of available and emerging structural data on alternative oligomeric states indicates that the same principles may also apply to the subunit number of other circular assemblies suggesting that the deletion/addition approach could be used generally to engineer transitions between different oligomeric states
First Crystal Structure for a Gold Carbene-Protein Adduct
The X-ray structure of the adduct formed in the reaction between the gold N-heterocyclic carbene compound Au(NHC)Cl (with NHC = 1-butyl-3-methyl-imidazole-2-ylidene) and the model protein thaumatin is reported here. The structure reveals binding of Au(NHC)(+) fragments to distinct protein sites. Notably, binding of the gold compound occurs at lysine side chains and at the N-terminal tail; the metal binds the protein after releasing Cl- ligand, but retaining NHC fragment
The Crystal Structure of Tobermorite 14 Ã… (Plombierite), a C-S-H phase
The crystal structure of tobermorite 14 Å (plombierite) was solved by means of the application of the order-disorder (OD) theory and was refined through synchrotron radiation diffraction data. Two polytypes were detected within one very small crystal from Crestmore, together with possibly disordered sequences of layers, giving diffuse streaks along c*. Only one of the two polytypes, could be refined: it has B11b space group symmetry and cell parameters a = 6.735(2) Å, b = 7.425(2) Å, c = 27.987(5) A, γ = 123.25(1)° . The refinement converged to R = 0.152 for 1291 reflections with F 0>4σ(F 0). The characteristic reflections of the other polytype, F2dd space group, a ≈11.2 Å, b ≈ 7.3 Å, c ≈ 56 Å, were recognized but they were too weak and diffuse to be used in a structure refinement. The structure of tobermorite 14 Å is built up of complex layers, formed by sheets of sevenfold coordinated calcium cations, flanked on both sides by wollastonite-like chains. The space between two complex layers contains additional calcium cations and H 2O molecules; their distribution, as well as the system of hydrogen bonds, are presented and discussed. The crystal chemical formula indicated by the structural results is Ca 5Si 6O 16(OH) 2 ·7H 2O
Differences in run-up, take-off, and flight characteristics: successful vs. unsuccessful high jump attempts at the IAAF world championships
Studies previously conducted on high jump have yielded important information regarding successful performance. However, analyses in competitive scenarios have often disregarded athletes’ unsuccessful attempts. This study aimed to investigate the biomechanical differences between successful and unsuccessful jumps during competition. High-speed video footage (200 Hz) was obtained from 11 athletes during the 2018 Men's World Athletics Indoor Championship Final. From each athlete, one successful (SU) and one unsuccessful (UN) jump at the same bar height were included in the analysis, leaving seven athletes in total. Following whole-body 3D manual digitization, several temporal and kinematic variables were calculated for the run-up, take-off, and flight phases of each jump. During SU jumps, athletes raised the center of mass to a greater extent (p < 0.01) from take-off. Touchdown in SU jumps was characterized by a faster anteroposterior velocity (p < 0.05), lower backward lean (p < 0.05), and changes in joint angles for the stance and trail limbs (p < 0.05). Athletes also shortened the final contact time during SU jumps (p < 0.01) after producing a longer flight time in the final step of the run-up (p < 0.05). Elite-level high jumpers undertake a series of adjustments to successfully clear the bar after UN jumps. These adjustments reinforce the importance of the run-up in setting the foundations for take-off and bar clearance. Furthermore, the findings demonstrate the need for coaches to be mindful of the adjustments required in stance and trail limbs when looking to optimize feedback to athletes during training and competition
Crystal structure of afghanite, the eight-layer member of the cancrinite-group: Evidence for long-range Si,Al ordering
Afghanite, ideally [(Na,K)(22)Ca-10][Si24Al24O96](SO4)(6)Cl-6, is the eight-layer member of the cancrinite-group (ABABACAC stacking sequence). Its structure was refined in the P31c space group to R = 4.5% by means of single-crystal X-ray diffraction data. The cell parameters are a = 12.8013(7) Angstrom, c = 21.4119(18) Angstrom. The P6(3)mc space group proposed in a previous structure refinement is not consistent with the ordered Si,AI pattern suggested by an Si/Al ratio equal to 1 shown by afghanite and other members of the cancrinite-group. The Si-O and Al-O bond distances, 1.61(2) Angstrom and 1.72(2) Angstrom respectively, found in the structure refinement, are in accordance with an ordered Si,AI distribution which is allowed by the P31c space group, a maximal non isomorphic subgroup of P6(3)mc. Afghanite contains six 11-hedra (cancrinite) cages and two 23-hedra (liottite) cages. Four cancrinite cages are stacked along [0 0 z]. They contain a regular....Ca-Cl-Ca-Cl.... chain similar to that observed in davyne and related phases: in particular Ca is located near the center of the bases whereas Cl is near the center of the cage. A liottite cage with a base-sharing cancrinite cage is stacked along [2/3 1/3 z] and [1/3 2/3 z]. The liottite cage hosts a maximum of three sulphate groups which alternate regularly with cation-containing planes. The cancrinite cage, that shares the bases with the liottite cages, presents a disordered distribution of Cl and F reading to two possible configurations similar to those observed in liottite
A Study on Perceptions towards Organic and Local Production, and Individuals’ Socio-Demographic and Geographical Affiliation Influencing Fruit and Vegetable Purchasing Preferences of EU Households
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