A Change in the Radius of Rotation of F1-ATPase Indicates a Tilting Motion of the Central Shaft

AbstractF1-ATPase is a water-soluble portion of FoF1-ATP synthase and rotary molecular motor that exhibits reversibility in chemical reactions. The rotational motion of the shaft subunit γ has been carefully scrutinized in previous studies, but a tilting motion of the shaft has never been explicitly postulated. Here we found a change in the radius of rotation of the probe attached to the shaft subunit γ between two different intermediate states in ATP hydrolysis: one waiting for ATP binding, and the other waiting for ATP hydrolysis and/or subsequent product release. Analysis of this radial difference indicates a ∼4° outward tilting of the γ-subunit induced by ATP binding. The tilt angle is a new parameter, to our knowledge, representing the motion of the γ-subunit and provides a new constraint condition of the ATP-waiting conformation of F1-ATPase, which has not been determined as an atomic structure from x-ray crystallography

Parity Violation in Neutron Resonances of Palladium

Parity violation in p-wave neutron resonances of the palladium isotopes 104, 105, 106, and 108 has been measured by transmission of a longitudinally polarized neutron beam through a natural palladium target. The measurements were performed at the pulsed spallation neutron source of Los Alamos Neutron Science Center. The rms weak interaction matrix elements and the corresponding spreading widths were determined for 104 Pd, 105 Pd, and 106 P

Neutron Resonance Spectroscopy of 104Pd, 105Pd, and 110Pd

We have measured neutron resonances in the palladium isotopes 104, 105, and 110 for neutron energies from 1 to 2100 eV. Many new p-wave resonances have been observed. Their neutron widths and, in several cases, the radiative widths were measured. The average level spacings and the s-wave and p-wave neutron strength functions were determined. The time-of-flight method was used for both neutron total cross section measurements and total (n,γ) reaction yield measurements at the pulsed spallation neutron source of Los Alamos Neutron Science Center. Well established resonance spectroscopy for these isotopes is essential for the analysis of parity violation data that were recently measured in palladium

Neutron Resonance Spectroscopy of 103Rh from 30 eV to 2 keV

Neutron resonances in 103Rh have been measured for neutron energies from 30 to 2000 eV using the time-of-flight method and the (n,γ) reaction. The rhodium resonance spectroscopy is essential for the analysis of parity violation measurements recently performed on neutron resonances in 103Rh. Neutron scattering and radiative widths were determined, and orbital angular momentum assignments made with a Bayesian analysis. The s-wave and p-wave strength functions and average level spacings were determined

Parity Violation in Neutron Resonances of 103Rh

Parity nonconservation (PNC) was studied in p-wave neutron resonances of 103Rh in the neutron energy range 30 to 490 eV. The helicity dependence of the neutron total cross section of rhodium was determined by capture measurements with the time-of-flight method at the Manuel Lujan Neutron Scattering Center at the Los Alamos National Laboratory. A total of 32 p-wave resonances were studied and statistically significant longitudinal asymmetries were observed for resonances at En=44.5, 110.8, 321.6, and 432.9 eV. A statistical analysis treating the PNC matrix elements as random variables yields a weak spreading widthΓw=(1.42-0.59+1.21)×10-7eV

Neutron Resonance Spectroscopy of 117Sn from1 eV to 1.5 keV

Parity violation has been studied recently for neutron resonances in 117Sn. The neutron resonance spectroscopy is essential for the analysis of the parity violation data. We have measured neutron resonances in 117Sn for neutron energies from 1 to 1500 eV using the time-of-flight method and the (n,γ) reaction. The sample was enriched to 87.6% 117Sn. Neutron scattering and radiative widths were determined, and orbital angular momentum assignments were made with a Bayesian analysis. The s-wave and p-wave strength functions and average level spacings were determined

Parity Nonconservation in Neutron Resonances in 133Cs

Spatial parity nonconservation (PNC) has been studied in the compound-nuclear states of 134Cs by measuring the helicity dependence of the neutron total cross section. Transmission measurements on a thick 133Cs target were performed by the time-of-flight method at the Manuel Lujan Neutron Scattering Center with a longitudinally polarized neutron beam in the energy range from 5 to 400 eV. A total of 28 new p-wave resonances were found, their neutron widths determined, and the PNC longitudinal asymmetries of the resonance cross sections measured. The value obtained for the root-mean-square PNC element M=(0.06-0.02+0.25) meV in 133Cs is the smallest among all targets studied. This value corresponds to a weak spreading width Γw=(0.006-0.003+0.154)×10-7 eV

Parity Violation in Neutron Resonances of 117 Sn

Parity nonconservation (PNC) has been studied in neutron p-wave resonances of 117Sn. The longitudinal asymmetries were measured for 29 p-wave resonances in the neutron energy range 0.8 eV to 1100 eV. Statistically significant PNC effects were observed for four resonances. A statistical analysis determined the rms weak mixing matrix element and the weak spreading width. A weak spreading width of Γw=(0.28-0.15+0.56)×10-7 eV was obtained for117Sn

Measurement of parity-nonconserving rotation of neutron spin in the 0.734-eV p-wave resonance of 139La^{139}La

The parity nonconserving spin rotation of neutrons in the 0.734-eV p-wave resonance of $^{139}La$ was measured with the neutron transmission method. Two optically polarized $^3He$ cells were used before and behind a a 5-cm long $^{139}La$ target as a polarizer and an analyzer of neutron spin. The rotation angle was carefully measured by flipping the direction of $^3He$ polarization in the polarizer in sequence. The peak-to-peak value of the spin rotation was found to be $(7.4 \pm 1.1) \times 10^{-3}$ rad/cm which was consistent with the previous experiments. But the result was statisticallly improved. The s-p mixing model gives the weak matrix element as $xW = (1.71 \pm 0.25)$ meV. The value agrees well with the one deduced from the parity-nonconserving longitudinal asymmetry in the same resonance

Neutron Resonance Spectroscopy of 106Pd, and 108Pd from 20–2000 eV

Parity nonconserving asymmetries have been measured in p-wave resonances of 106Pd and 108Pd. The data analysis requires knowledge of the neutron resonance parameters. Transmission and capture γ-ray yields were measured for En=20–2000 eV with the time-of-flight method at the Los Alamos Neutron Science Center (LANSCE). A total of 28 resonances in 106Pd and 32 resonances in 108Pd were studied. The resonance parameters for 106Pd are new for all except one resonance. In 108Pd six new resonances were observed and the precision improved for many of the resonance parameters. A Bayesian analysis was used to assign orbital angular momentum for the resonances studied