A deep narrowband survey for planetary nebulae at the outskirts of M33

Context: Planetary nebulae (PNe) are excellent tracers of stellar populations with low surface brightness, and therefore provide a powerful method to detect and explore the rich system of substructures discovered around the main spiral galaxies of the Local Group. Aims: We searched the outskirts of the Local Group spiral galaxy M33 (the Triangulum) for PNe to gain new insights into the extended stellar substructure on the northern side of the disc and to study the existence of a faint classical halo. Methods: The search is based on wide field imaging covering a 4.5 square degree area out to a maximum projected distance of about 40 kpc from the centre of the galaxy. The PN candidates are detected by the combination of images obtained in narrowband filters selecting the [OIII]$\lambda5007\AA$ and H$\alpha$ + [NII] nebular lines and in the continuum g' and r' broadband filters. Results:Inside the bright optical disc of M33, eight new PN candidates were identified, three of which were spectroscopically confirmed. No PN candidates were found outside the limits of the disc. Fourteen additional sources showing [OIII] excess were also discovered. Conclusions:The absence of bright PN candidates in the area outside the galaxy disc covered by this survey sets an upper limit to the luminosity of the underlying population of $\mathrm{\sim1.6\cdot10^{7}L_{\odot}}$, suggesting the lack of a massive classical halo, which is in agreement with the results obtained using the RGB population.Comment: 13 pages, 18 figure

New candidate planetary nebulae in M81

A 34 x 34 arcmin field centred on the spiral galaxy M81 has been searched for emission-line objects using the prime focus wide field camera (WFC) of the 2.54 m Isaac Newton Telescope (La Palma, Spain). A total of 171 candidate planetary nebulae (PNe) are found, 54 of which are in common with the ones detected by Jacoby et al. (1989). The behaviour of PNe excitation as a function of galactocentric distance is examined, and no significant variations are found. The PNe luminosity function is built for the disk and bulge of M81, separately. A distance modulus of 27.92+-0.23 mag is found for disk PNe, in good agreement with previous distance measurements for M81 (Jacoby et al. 1989; Huterer et al. 1995).Comment: 7 pages including 2 tables. A&A accepted; also available at http://www.iac.es/publicaciones/preprints.htm

The S2N2 metallicity calibrator and the abundance gradient of M 33

We introduce the log(Ha/[SII]6717+6731) vs. log(Ha/[NII]6583) (S2N2) diagnostic diagram as metallicity and ionisation parameter indicator for HII regions in external galaxies. The location of HII regions in the S2N2 diagram was studied both empirically and theoretically. We found that, for a wide range of metallicities, the S2N2 diagram gives single valued results in the metallicity-ionisation parameter plane. We demonstrate that the S2N2 diagram is a powerful tool to estimate metallicities of high-redshift (z ~ 2) HII galaxies. Finally, we derive the metallicity for 76 HII regions in M33 from the S2N2 diagram and calculate an O/H abundance gradient for this galaxy of -0.05 (+-0.01) dex kpc^-1.Comment: 10 pages, 3 figures and 2 tables. Accepted for publication in MNRA

Chemical Abundances of Planetary Nebulae in M33

Using spectroscopic data presented in Magrini et al. (2003), we have analyzed with the photoionization code CLOUDY 94.00 (Ferland et al. 1998) 11 Planetary Nebulae belonging to the spiral galaxy M 33. Central star temperatures and nebular parameters have been determined. In particular the chemical abundances of He/H, O/H, N/H, Ar/H, and S/H have been measured and compared with values obtained via the Ionization Correction Factors (ICFs) method, when available. Chemical abundance relationships have been investigated; in particular, a correlation between N/H and N/O similar to the Galactic one (Henry 1989), and a feeble anti-correlation between O/H and N/O have been found. A gradient in O/H across the disc of M~33 is indicatively consistent with the one found from HII regions in this galaxy (Vilchez et al 1988). Further studies in the more external parts of M~33 are however needed to ascertain this point. The present result shows that oxygen and helium abundances (with lower accuracy also nitrogen, argon and sulphur) can be actually estimated from the brightest PNe of a galaxy, even if the electron temperature cannot be measured. We also found that the oxygen abundance is quite independent of the absolute magnitude of the PN and consequently the brightest PNe are representative of the whole PN population. This represents an important tool to measure the metallicity of galaxies at the time of the formation of PNe progenitors.Comment: 17 pages, 6 figures, A&A accepte

Abrogation of mercuric chloride-induced nephritis in the Brown Norway rat by treatment with antibodies against TNFα

HgCl2 induces an autoimmune disease in the Brown Norway rat characterized by synthesis of autoantibodies (mainly, anti-GBM Abs), severe proteinuria and interstitial nephritis. Also, HgCl2- injected rats develop glomerular cell infiltrates consisting of ED1+ cells (monocyte/macrophage), starting on day 4 and reaching a maximum on day 8. Treatment with anti-TNF-α antiserum had preventative effects as it reduced the urinary protein levels to close to the normal range and also blocked the influx of inflammatory cells in the renal glomeruli and interstitium, but circulating anti-GBM and lineal glomerular IgG deposits were unmodified. In addition, whole isolated glomeruli from HgCl2-induced nephritis secreted TNF-α commencing on day 8, being maximally detected on day 11 and preceding, between 2 to 3 days, the development of proteinuria. The administration of anti-TNF-α antiserum or anti-α4 integrin mAb completely abrogated the synthesis of TNF-α in glomeruli isolated from the respective treated groups of animals, in addition to the proteinuria. Taken together our results confirm that TNF-α plays an important role in the induction and development of HgCl2-induced nephritis and highlights the pathogenic importance of the local release of TNF in those renal diseases in which prominent glomerular macrophage accumulation is a constant feature