Coupling between Rydberg states and Landau levels of electrons trapped on liquid helium

We investigate the coupling between Rydberg states of electrons trapped on a liquid Helium surface and Landau levels induced by a perpendicular magnetic field. We show that this realises a prototype quantum system equivalent to an atom in a cavity, where their coupling strength can be tuned by a parallel magnetic field. We determine experimentally the renormalisation of the atomic transition energies induced by the coupling to the cavity, which can be seen as an analogue of the Lamb shift. When the coupling is sufficiently strong the transition between the ground and first excited Rydberg states splits into two resonances corresponding to dressed states with vacuum and one photon in the cavity. Our results are in quantitative agreement with the energy shifts predicted by the effective atom in a cavity model where all parameters are known with high accuracy

Cell-marking techniques for cell lineage tracing

A zygote, the only totipotent cell of the developing organism, transforms into a complex, multicellular entity with billions (for humans) of highly specialized cells and tissues. Most adult tissues are maintained by a combination of highly dynamic processes of senescence, apoptosis and rejuvenation with new cells constantly arising from dispersed depots of stem cells. Studying individual cell fates and their intertwined relations thus aids in understanding ontogenetic development as well as pathogenic processes in the body. Direct observations of developing embryos uncovered the fate of single blastomeres of ascidia and nematode. In both cases, research benefited from the simplicity of these objects, because, in ascidia, each blastomere has unique signatures naturally, and, in nematode, transparency of a worm’s 959-cell body allows every cell to be traced through development individually. In most cases, however, studying cellular lineages and identification of stem cells’ subpopulations are a true challenge for investigators. To trace the cell’s fate, novel methods were invented that introduce special tags into cells, the tags that would be inherited during cell divisions. Every descendent of a marked cell bears the same tag and can easily be distinguished from unrelated cellular neighbors. This review focuses on modern methods for cell tracing with dyes and genetic constructs encoding protein reporters that mark cell lineages. Special focus is on genome-integrated tags (genetic labeling), such as viral and cellular barcoding. One chapter of the review describes novel advancements in the field of CRISPR/Cas9-based cellular barcoding

Rehabilitation for nephroptosis

The restoration of physical health became possible with the development of rehabilitation. The article discusses the rehabilitation of nephroptosis with the help of physical therapy. Physical education not only helps to keep fit, but also is indispensable in the rehabilitation of diseases.Восстановление физического здоровья стало возможным с развитием реабилитации. В статье рассматривается реабилитация при нефроптозе при помощи лечебной физкультуры. Занятие физической культурой не только помогают поддерживать форму, но и являются незаменимыми в реабилитации заболевани

Improving Health Industries by the Supply-Chain Policy

Abstract— the article deals with issues of the policy in the sphere of protection of a Supply-Chain management’s rights and legitimate interests in the health centres. The issues of protection of the rights of Supply-Chain management in the field of health care revealed. In today's global and national economy, businesses are increasingly relying on outsourcing some of their activities and processes. Therefore, more and more companies at the Supply-Chain level, in specific networks, operate and compete with their suppliers and service providers. This process of outsourcing and the growing importance of supply chains has its consequences in the working conditions and health and safety of workers of supply companies and contractors. There are two main networks of Supply-Chain or relationships between companies and members of their supply chain: the main network (a company and suppliers of its specific goods and materials) and the secondary network (a company and its contractors and subcontractors special services such as activities Maintenance, construction, cleaning or catering This report provides an overview, analysis, conclusions and recommendations on how to upgrade health centers through the above Supply-Chain networks

Photoconductivity of SrAlF5 crystals doped with Ce3+ ions

© Published under licence by IOP Publishing Ltd. Temporal behaviour of the complex permittivity of Ce:SrAlF5 and Ce,Yb:SrAlF5 crystals under laser irradiation in 240-280 nm spectral range was investigated by a microwave resonance technique. The photoconductivity spectrum of these crystals was detected. It was established that photoconductivity signal of the samples in 240-280 nm spectral range is caused by two processes: excited-state absorption of Ce3+ ions and photoionization of color centers

Chemotherapy changes cytotoxic activity of NK-cells in cancer patients

In recent years, it has been shown that under certain conditions cytostatic agents (chemotherapy and radiotherapy) can restore the functioning of the immune system impaired by malignancy burden. The modifications of biological properties by cytostatics acting make cancer cells visible for the immune system recognition and elimination. Eighteen patients diagnosed with primary local breast (8) and gastric (10) cancer between 2014 and 2016 were enrolled in the investigation. The phenotypic features of NK were assessed by flow cytometry using mAb (BD Pharmingen) against CD45 (common leukocyte antigen) and CD56 (NK-marker) for surface staining, CD107a (LAMP-1), Perforin (PF) and Gransime B (GB) for intracellular staining. We examined NK populations in the peripheral blood of cancer patients before treatment and in 5 days after second course of NACT. We found that NK populations produced PF in cancer patents, which were absent before treatment, increased after NACT. Their emergence can be associated with the immunoactivating effects of chemotherapy, realized by the modification of tumor cells or elimination of immunosuppressive cells

Spectral-kinetic studies of SrAlF 5 doped by trivalent rare-earth ions

Photochemical properties of Ce 3:SrAlF 5 and Ce 3,Yb 3:SrAlF 5 single crystals together with spectroscopic and kinetic characteristics of several optically nonequivalent impurity centers and energy transfer between them are described. It is shown that co-activation by Yb 3 ions effectively suppresses color centers in Ce,Yb:SAF crystals. It was found out that in Ce,Yb:SAF crystals Yb ions exist simultaneously in 2 and 3 valent state. Three types of optically nonequivalent luminescent centers corresponding to the doublets in luminescence spectrum centered at 290, 305 and 370 nm (Ce I, Ce II, Ce III, respectively) have been observed. Analysis of luminescence spectra and decays leads to the conclusion that there is no energy transfer between either cerium centers or from Ce 3 to Yb 2 apart from the Ce III center which luminescence is slightly quenched by Yb 2. © 2012 Elsevier B.V. All rights reserved

CRISPR/Cas9, a universal tool for genomic engineering

The CRISPR/Cas9 system was initially described as an element of archeal and bacterial immunity, but gained much attention recently for its outstanding ability to be programmed to target any genomic loci through a short 20-nucleotide sgRNA region. Here we review some modern applications of the CRISPR/Cas9 system. First, we describe the basic mechanism of the CRISPR/Cas9 DNA recognition and binding, focusing in particular on its off-target activity. The CRISPR/Cas9 off-target activity refers to a non-specific recognition of genomic sites that have partial homology with sgRNA, occasionally resulting in unwanted mutations throughout the genome. We also note some recent improvements for enhancing Cas9 specificity or adding new functions to the system. Since Cas9-related hype is mostly driven by its remarkable potential for gene therapy and genome engineering, the latest CRISPR/Cas9 applications in these areas are also covered in our review. For instance, the CRISPR/Cas9 was recently used to control HIV infection and to repair genetic abnormalities, such as Duchenne muscular dystrophy or retinitis pigmentosa, both in cell cultures and rodent models. A programmable nature of CRISPR/Cas9 facilitates the creation of transgenic organisms through sitespecific gene mutations, knock-ins or large chromosomal rearrangements (deletions, inversions and duplications). CRISPR/Cas9 proved to be especially useful in pronuclear microinjections of farm animals as well, having strong impact on biotechnology. In addition, we review Cas9-augmented genetic screens that allow an unbiased search for new genes and pathways involved in a plethora of biological aspects, owing to Cas9 efficiency and versatility. Finally, we argue that gene drivers based on CRISPR/ Cas9 represent a powerful tool to modify ecosystems in the nearest future