Effect of time, moisture content, and substrate amount on sorbitol production using entrapment of Lactobacillus plantarum (BAA-793) in sodium alginate beads

Agro-industrial wastes such as Meranti wood sawdust (MWS) have great potential as substrates for the production of sorbitol and other biochemical products. In this study, treated cellulose from MWS was used as a substrate to produce sorbitol via solid state fermentation (SSF) process using immobilized cells of Lactobacillus plantarum strain (BAA-793), entrapped in sodium alginate. The effect of fermentation time, moisture content, and substrate amount on sorbitol concentration were studied at the following ranges (fermentation time: 2–8 h, moisture content: 40–80%, and substrate amount: 0.5–2.5 g). The results show that the fermentation time of 4 h, substrate amount of 2 g and moisture content of 50% yielded 8.396 g/L of sorbitol. With a moisture content of 50%, substrate amount of 2 g, and fermentation time of 6 h yielded 4.726 g/L of sorbitol. The highest concentration of sorbitol (13.607 g/L) was obtained at the optimized condition of fermentation time 4 h, moisture content 50%, and substrate amount 1.0 g. These results indicate that the fermentation time, moisture content, and substrate amount are important factors to be considered in order to achieve high sorbitol yield

Influenced factors in the delignification process of red meranti wood sawdust

Lignocellulosic biomass are plants that include forestry residue and agricultural residues that are mainly composed of cellulose, hemicelluloses and lignin. Red Meranti wood sawdust (RMWS) are one of lignocellulosic biomass that rich-cellulose content. To obtain cellulose, the pretreatments are needed to extract it from outer layer of lignin and hemicellulose by using the acid-chlorite delignification procedure aided with design of experimental from Design Expert 7.1 software. Four factors were selected in design of experiment using two level with half fraction factorial analysis were came out with total of 8 runs. The factors contributed were ratio acetic acid (AC) to RMWS (0.45 and 0.6), ratio sodium chlorite to RMWS (0.6 and 1.64), reaction time (4hr and 6hr) and temperature (55°C and 75°C). The results obtained were showed that the design model was substantial resulting with a coefficient of determination value of 0.9963. Two factors that generated the highest to the process were ratio SC to RMWS (B) and temperature (D). The percentage error between the actual and predicted value for lignin removal at 0.79% and 4.92%, which found to be less than 5%, and thus, the model was successfully validated

Mixed Reverse Micellar Systems (MRMS) for Extraction of Erythromycin: Effect of Surfactant Concentration on Forward Extraction Step

Mixed reverse micellar systems (MRMS) of sodium bis(2-ethylhexyl)sulfosuccinate (AOT) and zwitterionic surfactants 3-(N,N-Dimethyloctadecylammonio) propanesulfonate (SB3-18) in iso-octane were used for the forward extraction of erythromycin from the aqueous phase to organic phase for the first time. The effect of surfactant concentration on the percentage of erythromycin transferred was investigated in detail. It was found that sufficient AOT (20 g/L) concentration was required for the solubilising of erythromycin in MRMS. Erythromycin was successfully solubilised in MRMS with higher percentage of erythromycin transfer which was 98.13% at 60.0 g/L of AOT concentration

Effect of Process Parameters on Pectin Extraction from Dragon Fruit (Hylocereus polyrhizus) Peels via Chemical and Physical Treatment

Dragon fruit (Hylocereus polyrhizus) peel was found to be one of the potential sources of pectin. The combination of physical and chemical treatments namely mild ultrasound, stirring and citric acid has been applied with the aim to get the high yield of pectin during extraction. The influence of several process namely agitation, temperature, time, pH and liquid solid ratio during extraction process were investigated. The one factor at a time (OFAT) method was used to determine the possible best levels of each factor during extraction. The pectin obtained from these experiments was compared in term of yield based on dry weight. The highest yield of pectin 42.5% (w/w) was obtained when the extraction was carried out at agitation, temperature, time, pH and solid liquid ratio of 250 rpm 70oC, 120 min, pH of 1.5 and 1:10 (w/v), respectively

Effects of Solvents on the Amount of Anthraquinone

Dyes derived from natural sources have appeared as a significant alternative to synthetic dyes. Recently, the scientific community has begun to show interest in Morinda citrifolia as it is one of the natural sources. The roots of these plants were reported to be a good source of important compounds, known as anthraquinones, which have been proved an important naturally occurring pigments that are widely distributed in nature to produce yellow or red dyes. However, it is not known which method and types of solvent will obtain the highest anthraquinone extract from Morinda citrifolia. In order to tackle this problem, this research was carried out to study effects of solvents on the concentration of anthraquinone using solvent extraction method at room temperature for 10 hour. Ethanol, acetone and acetonitrile were types of solvent used. The anthraquinone extract was analyzed using UV-Vis Spectrophotometer afterwards. It was observed that the acetone is the best solvent to extract the highest anthraquinone concentration followed by ethanol and acetonitrile respectively. Acetone gave anthraquinone concentration about 0.0844g/L, while ethanol and acetonitrile was 0.0440g/L and 0.0355g/L correspondingly. It also shows that acetone gave the best color in which brick red color. The results suggested that the solvent extraction method able to yield colorants from Morinda citrifolia which can work out commercially, but the color varies based on the types of solvent used

Regression Analysis for the Adsorption Isotherms of Betacyanin Extracts from the Dragon Fruit Peel onto the Spun Silk Yarn

The betacyanin pigment extracted from the dragon fruit peel has a potential to be a natural dye as an alternative to replace the synthetic dyes. To investigate the dyeability of spun silk with betacyanin pigment, the adsorption isotherm models were performed. The equilibrium adsorption data were analyzed using the Langmuir, Freundlich, and Temkin isotherm models. In order to determine the best-fit isotherm for each system, three error analysis methods were used to evaluate the data, namely the sum of the squares of the errors, residual root mean square error and chi-square test. On the basis of low three error analysis and high correlation of determination, it was found that the Langmuir isotherm model fitted well with the experimental data. Therefore, it can be concluded that the adsorption process of betacyanin pigment onto the spun silk followed the Langmuir isotherm model. Moreover, the adsorption features of the experimental system might be caused by the monolayer adsorption

Production of Biodiesel using Tannery Fleshing as a Feedstock via Solid-state Fermentation

The objective of this study is to investigate solid state fermentation (SSF) mediated hydrolysis of tannery wastes to produce short and long chain fatty acids (SCFA and LCFA) followed by transesterification with methanol using alkali catalyst. Controlled inoculum which was isolated from soak liquor to be used in SSF, showed the maximum homology with the Microbacterium species by 16srDNA sequencing method. At 72 hours of Solid state fermentation (SSF), the maximum acidity and triglyceride hydrolysis were 39.46 % and 83.9% respectively. The predominant total fatty acids of the SSF samples were found to be oleic acid (C18:1n-9), palmitic acid (C16:0), and stearic acid (C18:0) and estimated to be 80 %. Transesterification rate was optimized at 3 hr reaction time with 1%NaOH (w/v %), 6% methanol to oil ratio (w/v %) to produce 89% conversion rate. C13 NMR confirmed the formation of fatty acid methyl esters group in the oil

High methoxyl pectin extracts from hylocereus polyrhizus's peels: Extraction kinetics and thermodynamic studies

The effect of physicochemical treatment on pectin yield, degree of esterification, along with the kinetics and thermodynamics characteristics was investigated in the present study. Several extraction parameters were observed to have impacted the yield and degree of esterification significantly, and the best extraction condition was as follows: agitation rate of 250 rpm, temperature of 70 °C, extraction time of 120 min, pH 2, and liquid to solid ratio of 10 v/w which has resulted in 28.20% of pectin yield, with DE (degree of esterification) of 57.00%. A theoretical model which describes the extractability, dissolution and degradation rate of pectin to predict the maximal yield at the maximal time was established to study the extraction kinetics of pectin from HPP. The kinetic analysis from Panchev's model shows the extraction rate was found highest at LSR 10 with ymax 30.85%. The calculated activation energy for pectin dissolution and degradation was found to be 4.532 kJ/mol and 28.054 kJ/mol, respectively. The thermodynamic study has suggested that the process was endothermic, spontaneous and reversible. These results suggest that the physical and chemical treatment applied could be an efficient technique for the extraction of pectin from Hylocereus polyrhizus peels

Process Enhancement of Hydrogen And Methane Production from Palm Oil Mill Effluent Using Two-Stage Thermophilic and Mesophilic Fermentation

The present study investigates the technical possibilities of hydrogen and methane production from palm oil mill effluent (POME). The production was carried out in two stage (thermophilic and mesophilic) continuous phase with recirculation of the digestate sludge. The reactors used for the present study, up-flow anaerobic sludge blanket reactor (UASB) and continuous stirred tank reactor (CSTR) were operated under thermophilic and mesophilic conditions, respectively. The UASB reactor was operated at 2 days hydraulic retention time (HRT) and 75 kgCOD m3 d−1 organic loading rate (OLR) for hydrogen production. The effluents from UASB reactor containing mainly with acetate and butyrate were directly fed into CSTR for methane production and 5 days HRT was maintained. Both UASB and CSTR reactors were operated for 120 days continuously, and a stable production of the hydrogen and methane was obtained in the separate reactors. The maximum hydrogen and methane production rate achieved was 1.92 L H2 L−d−1 and 3.2 L CH4 L−1 d−1, respectively. The cumulative hydrogen and methane yields were 215 L H2/kgCOD−1 and 320 L CH4/kgCOD−1, respectively with the total COD removal efficiency of 94%. Thermoanaerobacterium species was dominant in hydrogen reactor, while methane reactor was dominated with Methanobrevibacter sp

Fermentative Hydrogen Production from Indigenous Mesophilic Strain Bacillus Anthracis PUNAJAN 1 Newly Isolated from Palm Oil Mill Effluent

In the present study, a new mesophilic bacterial strain, identified as Bacillus anthracis strain PUNAJAN 1 was isolated from palm oil mill effluent (POME) sludge, and tested for its hydrogen production ability. Effect of physico-chemical factors such as temperature, initial pH, nitrogen source and carbon sources were investigated in order to determine the optimal conditions for hydrogen production. The maximum hydrogen yield of 2.42 mol H2/mol mannose was obtained at 35 °C and initial pH of 6.5. Yeast and mannose were used as the main carbon and nitrogen sources respectively in the course of the hydrogen production. Apart from synthetic substrate, specific hydrogen production potentials of the strain using POME was calculated and found to be 236 ml H2/g chemical oxygen demand (COD). The findings of this study demonstrate that the indigenous strain PUNAJAN 1 could be a potential candidate for hydrogen using POME as substrate