Tools for in vitro propagation/synchronization of the liverwort Marchantia polymorpha and application of a validated HPLC-ESI-MS-MS method for glutathione and phytochelatin analysis

Bryophytes, due to their poikilohydric nature and peculiar traits, are useful and versatile organisms for studies on metal accumulation and detoxification in plants. Among bryophytes, the liverwort Marchantia polymorpha is an excellent candidate as a model organism, having a key role in plant evolutionary history. In particular, M. polymorpha axenic cultivation of gametophytes offers several advantages, such as fast growth, easy propagation and high efficiency of crossing. Thus, the main purpose of this work was to promote and validate experimental procedures useful in the establishment of a standardized set-up of M. polymorpha gametophytes, as well as to study cadmium detoxification processes in terms of thiol-peptide production, detection and characterisation by HPLC-mass spectrometry. The results show how variations in the composition of the Murashige and Skoog medium impact the growth rate or development of this liverwort, and what levels of glutathione and phytochelatins are produced by gametophytes to counteract cadmium stress

Examen del contenido en ascaridol del aceite esencial de Chenopodium Ambrosioides L. (Paico)

Se ha examinado el contenido en ascaridol de varias muestras de Chenopodium ambrosioides L., colectadas en zonas del noroeste de Corrientes (Argentina) mediante CG. El uso popular de las infusiones de paico en el conurbano de Buenos Aires en afecciones intestinales, especialmente parasitosis, ha llevado a frecuentes intoxicaciones en niños y aún en adultos dado el estrecho margen entre la dosis terapéutica y la tóxica. Plantas recogidas en Corrientes muestran que su contenido en ascaridol no supera el 10 % del total de la esencia, a diferencia de especies de otras provincias de la Argentina, con lo cual disminuye el riesgo en el uso de esta planta en la zona