2 research outputs found
Fenotipske promene izazvane imunizacijom encefalitogenom menjaju funkcije peritonealnih makrofaga u dva soja pacova razliÄite osetljivosti prema indukciji eksperimentalnog autoimunskog encefalomijelitisa (EAE).
We have investigated the phenotype of peritoneal cells and the functions of peritoneal macrophages obtained from experimental autoimmune encephalomyelitis (EAE)-susceptible Dark Agouti (DA) and EAE-resistant Albino Oxford (AO) rat strains on days 1, 3 and 7 post immunization with encephalitogen. Resident peritoneal cells from immunized and non-immunized rats of both strains were subjected to flow cytometric analyzes and after adherence were tested for zymosan phagocytosis, hydrogen peroxide (H2O2) and nitric oxide (NO) production. In non-immunized rats, macrophages from the DA rat strain phagocytosed more zymosan but produced less H2O2 than cells from the AO strain, while both strains produced comparable amounts of NO. Immunization increased phagocytosis in DA rats' cells, but decreased both phagocytosis and H2O2 production in cells from AO rats. Overall higher phagocyte ability in DA rats was associated with a significantly larger population of ED1+ cells (macrophages and dendritic cells), in contrast to a more pronounced expression of ED2 antigen (resident macrophages) on cells from AO rats. Immunization also increased the expression of CD11b molecule on non-resident ED2-macrophages of DA, but not of AO rats. The early and subtle phenotype changes in peritoneal cells of both rat strains might mirror the mechanism contributing to their different sensitivity to the induction of autoimmunity.Ispitivan je fenotip peritonealnih Äelija, kao i funkcije peritonealnih makrofaga, izolovanih od pacova Dark Agouti (DA) soja osetljivog na indukciju eksperimentalnog autoimunskog encefalomijelitisa (EAE) i pacova Albino Oxford (AO) soja koji je rezistentan prema EAE-u, 1, 3. i 7. dana nakon imunizacije encefalitogenom. Rezidentne peritonealne Äelije su ispitivane metodom protoÄne citofluorometrije, a zatim je nakon adherence testirana njihova sposobnost fagocitoze Äestica zimozana i kapacitet produkcije vodonik peroksida (H2O2) i azot monoksida (NO). U neimunizovanih pacova makrofage DA soja su intenzivnije fagocitovale Äestice zimozana i imale nižu sposobnost produkcije H2O2 nego Äelije pacova AO soja, ali nije bilo sojnih razlika u sposobnosti produkcije NO. Imunizacija je dovela do poveÄanja fagocitne sposobnosti makrofaga DA pacova, ali i do smanjenja fagocitoze i produkcije H2O2 makrofaga pacova AO soja. Generalno veÄu sposobnost fagocitoze u DA pacova prati i znaÄajno veÄa zastupljenost ED1+ Äelija (koje Äine uglavnom makrofage i dendritiÄne Äelije) nasuprot veÄoj zastupljenosti ED2 antigena (marker rezidentnih makrofaga) na Äelijama pacova AO soja. Imunizacija encefalitogenom je takoÄe dovela do poveÄanja ekspresije CD11b molekula na nerezidentnim ED2- Äelijama pacova DA, ali ne i AO soja. Rane i diskretne fenotipske promene na peritonealnim Äelijama pacova oba soja verovatno odslikavaju mehanizme koji doprinose njhovoj razliÄitoj osetljivosti prema indukciji autoimunskih oboljenja
Synthesis, Antimicrobial Activity and in silico Studies on Thymol Esters
Derivatisation of parent structure in terpenoids often results in enhancement of biological activity of newly obtained compounds. Thymol, a naturally occurring phenol biosynthesized through the terpene pathway, is a well known biocide with strong antimicrobial attributes and diverse therapeutic activities. We have aimed our study on a single modification of phenolic functionality in thymol in order to obtain a small focused library of twenty thymyl esters, ten of which were new compounds. All compounds were involved in in vitro antimicrobial testing. Another important aspect of current study was implementation of in silico calculation of physico-chemical, harmacokinetic and toxicological properties, which could be helpful by giving an additional guidance in further research