Specialties of Blood Serum Structurization Under Some Physiological Conditions

Background: it is well-known that physiological stress agents can to produce a significant response of regulatory systems. At the same time, the nature of metabolic shifts in the organism during significant physical exertion and emotional stress has been insufficiently studied in detail. Aim: The aim of this investigation is study of modification of crystallogenic properties of human blood serum action under physical exertion and psychoemotional stress. Results: it is stated that physical exertion and psychoemotional stress causes increasing of crystallogenic activity of human blood serum. This trend is more pronounced for the first impact. This is confirmed by the results of vizuametric analysis of blood serum facias and its spectrometric testing. Conclusion: we show that crystalloscopic picture of blood serum is an informative criterion of different physiological conditions, including physical exertion and psychoemotional stress. It realized in the shifts of structure of biological fluid facias

Effect of a glutathione-containing dinitrosyl iron complex on the oxidative metabolic state and crystallogenic properties of rat blood plasma: a preclinical experimental study

Background: The multifaceted regulatory role of nitric oxide in biological systems predetermines the high value of studying the possibilities of the external control of the compound level in organs and tissues. There are several fundamentally different ways of exogenous modulation of nitric oxide metabolism. The most promising option is the use of pharmacological donors. Dinitrosyl iron complexes (DNIC) with various ligands hold a prominent place among such donors as they are considered as a natural deposited form of nitric oxide.Objective. To study the effect of a glutathione-containing dinitrosyl iron complex on the oxidative metabolism parameters and crystallogenic activity of rat blood.Methods. A preclinical experimental randomized study was conducted on 60 sexually mature male Wistar rats weighing about 250 g. The animals were divided into 6 groups, each consisting of 10 individuals. Group 1 included intact (without any manipulations) individuals. In group 2, the rats were administered daily intraperitoneal injections of 1 ml. of 0.9% sodium chloride solution for 10 days. The rats included in the other four groups received daily intraperitoneal injections of 1 ml of dinitrosyl iron complexes with glutathione ligands in an isotonic sodium chloride solution with different agent concentrations: 0.15 mM for group 3; 0.30 mM for group 4; 0.45 mM for group 5; 0.60 mM for group 6. The final indicator of the study was the assessment of the oxidative potential and crystallogenic properties of blood under the conditions of administering various doses of glutathione-containing dinitrosyl iron complexes. The following parameters were used to assess the activity of proand antioxidant systems: lipid peroxidation intensity; the total activity of antioxidant systems, and malondialdehyde concentration. The parameters for intrinsic crystallization assessment included serum facies structural index, crystallizability, assessment of the marginal facies zone, and the destruction degree of facies elements. The obtained data calculation was performed using the software packages MS Office 2013 (Microsoft Corporation, USA) and Statistica 10 (StatSoft, USA).Results. The research established that glutathione-containing dinitrosyl iron complexes have an antioxidant effect. Moreover, the manifestation of these properties demonstrates a nonlinear dependence on their dose, with a possible optimum lying in the range of 0.3–0.45 mM. The study also revealed a tendency towards crystallogenic properties activation induced by this agent, corresponding to concentrations of 0.3 and 0.45 mM.Conclusion. The undertaken studies indicate the presence of an antioxidant effect in glutathione-containing dinitrosyl iron complexes. The manifestation of these properties demonstrates a dependence on their dose with a possible optimum varying from 0.3 to 0.45 mM. The research has established the activating effect of glutathione-containing dinitrosyl iron complex injections on the crystallogenic potential of the blood serum of healthy rats. This effect consisted in an increase in the density and complexity of crystalline elements. What is more, the maximal manifestation of this tendency (for metabolic indicators as well) corresponded to concentrations of 0.3 and 0.45 mM

ИССЛЕДОВАНИЕ ЗАВИСИМОСТИ КРИСТАЛЛОГЕННОЙ АКТИВНОСТИ БИОСРЕДЫ ОТ ИНТЕНСИВНОСТИ ЭКСПЕРИМЕНТАЛЬНОЙ ИНВАЗИИ Trichinella spiralis

Urine dehydration structurization at experimental trichinellosis of rats is studied. In 4 weeks after exposure rats urine was analyzed with biocrystallomics methods. It was stated, that urine crystallogenic properties changes according to intensity of infection.Изучена дегидратационная структуризация мочи крыс при экспериментальном заражении их Trichinella spiralis. Кристаллогенную и инициирующую активность мочи животных исследовали методом тезиокристаллоскопии через 4 недели после заражения. Исследование кристаллогенных свойств и инициаторного потенциала биологических жидкостей организма может стать индикатором наличия и глубины метаболических сдвигов, вызванных гельминтами

ДИНАМИКА НЕКОТОРЫХ ФИЗИКО-ХИМИЧЕСКИХ СВОЙСТВ СЛЮНЫ ПРИ ОПЕРАТИВНОМ ЛЕЧЕНИИ АЛЬВЕОКОККОЗА ПЕЧЕНИ

Dynamics of crystallogenic and initiating properties of saliva in patients with alveococcosis during the surgery and in the late postoperative period were specified. Saliva samples of 22 patients with liver alveococcosis were examined at the Kirov Zonal Hepathology Center. The diagnosis has been verified by instrumental methods (ultrasound examination, computer and/or magnetic-resonance tomography) and laboratory tests (latex agglutination test and enzyme-linked immunosorbent assay). A successful surgical treatment has been conducted in all patients. Crystal-logenic properties of saliva were estimated by the methods of crystalloscopy and thesigraphy along with a criteria-based definition of facies. A spectrometric examination of crystalloscopic and thesigraphic facies of saliva has also been conducted. It was determined that the surgical treatment of liver alveococcosis leads to a partial normalization of the crystallogenic and initiating properties of saliva already on checking out from hospital.Уточнена динамика кристаллогенной и инициирующей активности слюны пациентов с альвеококкозом при проведении оперативного лечения и в отдаленном послеоперационном периоде. Исследованы образцы слюны 22 пациентов с диагнозом альвеококкоз в Кировском зональном гепатологическом центре. Диагноз ставили на основании ультразвукового исследования, компьютерной и/или магнитно-резонансной томографии и лабораторных (латекс-агглютинации, ИФА) тестов. Всем больным выполнены оперативные вмешательства, которые они успешно перенесли. Кристаллогенные свойства слюны оценивали с использованием метода тезиокристаллоскопии с критериальным описанием фаций. Также проведено спектрометрическое исследование кристаллоскопических и тезиграфических фаций слюны. Установлено, что оперативное лечение альвеококкоза печени приводит к частичной нормализации кристаллогенных и инициирующих свойств слюны уже к моменту выписки из стационара

Microwave study of the effect of cold argon plasma on functional state of rat’s skin

The purpose of this study was a comparative study of the dielectric parameters of rat skin when treated with argon and argon cold plasma. The experiment was performed on 40 male Wistar rats divided into 4 equal groups. The first group of animals (n=10) was a control (intact). The rats of the remaining groups (n=10 in each) were treated daily with a pre-epilated area of the skin of the back (area = 1x1 cm). The duration of the course for all experienced groups is 10 procedures. Animals of the second group were treated with a non–ionized argon stream (the duration of one procedure was 1 minute), rats of the third and fourth groups were treated with argon cold plasma (1 and 2 minutes, respectively). Cold plasma generation was performed using a device using the principle of microwave ionization of a gas stream. Argon of high purity (99.99%) was used as the latter. The dielectric parameters of the skin of animals in the treated area (in control group rats – at a similar point in the back) were evaluated upon completion of a full course of exposure. For this purpose, a specialized software and hardware complex was used, providing near-field resonant microwave probing of biological tissues. It was found that the course treatment of the skin of the back of rats with gas streams with different ionization causes the formation of a specific functional-metabolic and morpho-structural response. Its character is determined by the parameters of the gas flow used: non-ionized argon significantly reduces the dielectric parameters (permeability and conductivity), and the result of the action of cold argon plasma depends on the exposure. During one-minute treatment, tissue permeability was observed to remain intact with a moderate decrease in conductivity. In the case of an increase in the exposure time to 2 minutes, the dielectric constant increased, and the conductivity remained unchanged

НЕКОТОРЫЕ КРИСТАЛЛОСКОПИЧЕСКИЕ СВОЙСТВА ДЕЗИНФЕКТАНТОВ И ПЕРСПЕКТИВЫ ПРИМЕНЕНИЯ КРИСТАЛЛОСКОПИЧЕСКОЙ ОЦЕНКИ В ДЕЗИНФЕКЦИИ И ДЕЗИНВАЗИИ

Simultaneous usage of microbiological, ecological and crystalloscopic methods can be used for estimation of disinfectants antibacterial and toxic activity. Disinfection action of the substance was made on the model of helminths eggs and pathogenic microorganisms (Staphylococcus aureus, Enterobacter aerogenes, Bacillus anthracis). Toxicity in vivo was estimated on mice urine.Одновременное применение микробиологических, экологических и кристаллоскопических методов можно использовать для оценки токсичности и антибактериальной активности дезинфектантов. Дезинфицирующее действие растворов азида натрия изучено на модели патогенных микроорганизмов (Staphylococcus aureus, Enterobacter aerogenes, Bacillus anthracis) и аборигенной микрофлоры. Токсичность азида натрия оценивали на основании анализа мочи мышей после его дачи

Морфофункциональные свойства стромальных стволовых клеток при культивировании in vitro в динамических условиях

The culture of stromal stem cells was incubated under constant and dynamic conditions. The incubation period was lasting 48 h. Intracellular enzyme activity of stromal stem cells were carried out with cytochemical methods. Alkaline phosphatase activity was increased under dynamic conditions, differentiation of stromal stem cells being accelerated comparatively steady-state conditions.Проведено культивирование стромальных клеток в динамических и стационарных условиях в течение 24-48 ч. Исследована их внутриклеточная ферментативная активность с помощью цитохимических методов - окраски на щелочную фосфатазу и неспецифическую эстеразу. В условиях динамического культивирования активность щелочной фосфатазы была выше по сравнению со стационарными условиями, что свидетельствует об ускорении дифференцировки и созревания стромальных клеток человека

Functional State of Rat's Erythrocytes Under Different Stress Conditions

Background: In our early publications was shown that electrophorhetic motility of erythrocytes (EPME) is a high effective criteria of adaptation response. This correlation is based on parallel development of adaptation syndrome and activation of the main organism regulatory systems, such as sympatoadrenalic and hypotalamo-hypophosial-adrenal ones. Objective: study of the influence of physical exercises and adrenaline injections on electrophorhetic motility, membrahes phospholipids spectrum and oxidative metabolism of the rats' erythrocytes. Methods: Rats were divided into three equal groups. First group of animals was control (n=10; without any manipulations). Rats of second group were subjected to physical load in the form of a sailing duration of 15 minutes with a cargo amounting to 10% of animal body weight (water temperature -26-28 0 C). Rats of third group were intraperitoneally injected with adrenaline hydrochloride (0.1 mg/kg). Blood sampling was made from the sublingual vein in 15, 30, 60, 120 minutes and 24 hours after exposure. We estimated the dynamics of the electrophorhetic motility of erythrocytes (EPME), the phospholipid spectrum of erythrocytes membranes, the concentration of malonic dialdehyde (MDA) and the state of the glutathione system. Results and conclusions: The study suggests that red blood cell as a biological system is capable for realization of stress response may develop a special "alarm reaction" after action of the stress agent. This response initiates activation of free radical processes and phospholipids profile in erythrocyte membranes with reducing of its electronegativity. This stage enhances the activity of the antioxidant system, is limiting the development of lipid peroxidation processes, and leads to the development of "adaptation stage" of the cellular system, coupled with the restoration of the electronegativity of the membrane and the mobilization of reserves of low molecular antioxidants, particularly glutathione, as well as "structural antioxidant", due to the content of readily oxidizable lipid (phosphoethanolamine) and lipids that are resistant to oxidation (phosphatidylcholine, sphingomyeline)

OXIDATIVE METABOLISM AND PHYSICAL PROPERTIES OF THE BLOOD IN ATHLETES

Objectives: Monitoring of metabolic status in athletes includes a wide range of different parameters, but not all of them have been fully studied. Thus, the presence of oxidative stress is noted in athletes, but its features are revealed only in fragments. The crystallogenic properties of blood in athletes have not been previously studied. Methods: The study enrolled a total of 262 athletes (19–29 years old) that are highly qualified in cyclic sports. Furthermore, screening data of apparently healthy non-exercising volunteers of the same age (n=35) were used for control. Blood serum of the athletes and non-exercising volunteers was analyzed for 8-isoprostane level, oxidized low-density lipoproteins, activity of superoxide dismutase, and glutathione peroxidase and reductase. We also studied the level α- and β-carotenes, α- and γ-tocopherols, lycopene, lutein, and zeaxanthin. Crystallogenic properties of blood serum were tested with own method. Results: The complex study demonstrated the presence of shifts in oxidative metabolism and blood serum physical and chemical properties induced by professional sports and manifested both in the status of blood pro- and antioxidative systems and in shifts of biofluids crystallogenic activity. At the same time, the obtained data confirmed the development of oxidative stress in qualified athletes. Conclusion: Our data shown that there are some shifts of oxidative metabolism and crystallogenic properties of blood plasma in professional athletes