PHA-Induced³H-Thymidine Stimulation and Urinary Arsenic (Inorganic + Organic) in Normal Human Blood Donors.

<p>Note: AsB = arseno betaine, MMA⁺⁵ and DMA⁺⁵ also include MMA⁺³ and DMA⁺³.</p><p>PHA-Induced³H-Thymidine Stimulation and Urinary Arsenic (Inorganic + Organic) in Normal Human Blood Donors.</p

Arsenite Suppresses Anti-CD3/Anti-CD28 Naïve HPBMC T cell Differentiation.

<p>Note: DP = double positive; Values shown are the Mean ± SD with *indicating statistical significance at p<.05.</p><p>Arsenite Suppresses Anti-CD3/Anti-CD28 Naïve HPBMC T cell Differentiation.</p

Evaluation of As⁺³ inhibition of HPBMC proliferation.

<p>In panel A, the typical response of 15 donors is shown where there is no inhibition of PHA-induced T cell proliferation. In panel B, 2 donors are shown that were extremely sensitive to low dose (0.1–1 nM) inhibition of the PHA response. Data shown are the Mean ± SD with *p<.05.</p

Correlation analysis of MMA in urine with PHA-induced T cell stimulation (³H-Thymidine counts per minute (CPM).

<p>There is an indication of a weak correlation between urinary MMA (combined MMA⁺³ and MMA⁺⁵) amounts and CPM in the 13 donors analyzed. Values are log transformed. R = −0.46, p = 0.24.</p

Proliferation response of PHA and anti-CD3/anti-CD28 stimulated PBMC treated with As⁺³.

<p>Comparison of PHA stimulated (A) and anti-CD3/anti-CD28 stimulated (B) T cell proliferation (³H-thymidine) for potential As⁺³ inhibition in 17 donors. Data shown are the Mean (red line) and Median (black line) ± SD with *p<.05.</p

30 Day Arsenic Drinking Water Exposure and Effects on Mouse Body Weight, Spleen Weight, Bone marrow Cell and Spleen Cell recovery¹

<p>30 Day Arsenic Drinking Water Exposure and Effects on Mouse Body Weight, Spleen Weight, Bone marrow Cell and Spleen Cell recovery^{<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0093920#nt101" target="_blank">1</a>}</p

<i>In vitro</i> (18 hrs) As⁺³, As⁺⁵ or MMA⁺³ Exposure and Effects on Mouse Bone marrow Cell Viability¹

<p><i>In vitro</i> (18 hrs) As⁺³, As⁺⁵ or MMA⁺³ Exposure and Effects on Mouse Bone marrow Cell Viability^{<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0093920#nt102" target="_blank">1</a>}</p

Suppression of T-dependent antibody response to sheep red blood cells post 30 day <i>in vivo</i> exposure of mice to As⁺³ via drinking water.

<p>After As⁺³ exposure, mice were immunized with SRBCs and examined for <i>in vitro</i> antibody production 4 days later. Results are Means <u>+</u> SD.</p

Number of colonies per million cells exposed to As⁺⁵ <i>in vitro</i>.

<p>Number of CFU-B colonies per million cells 10 days post plating in mouse methylcellulose media (containing As⁺⁵) for pre-B cells [Top]. Number of CFU-GM colonies per million cells 14 days post plating in mouse methylcellulose media (containing As⁺⁵) for GM cells [Bottom]. No significant differences between treatments and control (p<0.05) were found. Results are Means <u>+</u> SD.</p

Number of colonies per million cells exposed to MMA⁺³ <i>in vitro</i>.

<p>Number of CFU-B colonies per million cells 10 days post plating in mouse methylcellulose media (containing MMA⁺³) for pre-B cells [Top]. Number of CFU-GM colonies per million cells 14 days post plating in mouse methylcellulose media (containing MMA⁺³) for GM cells [Bottom]. *Significantly different compared to control (p<0.05). Results are Means <u>+</u> SD.</p