Syntheses of heterocyclic derivatives as potential cytotoxic compounds evaluated toward hepatocellular and cervical carcinoma cell lines

ABSTRACT. Through the present work the 3-oxo-N,3-diphenylpropamide derivatives 5a,b were used to synthesize pridine, pyrazole and thiophene derivatives. 3-Phenylisoxazol-5(4H)-one produced from the reaction of ethyl benzoylacetate was used as the key starting compound for different multi-component reactions. The synthesized compounds were evaluated toward Hepatocellular carcinoma HepG2 and cervical carcinoma HeLa cell lines. Compounds 3b, 5b, 7b, 7d, 9c, 9d, 15e, 15f, 16b, 18b, 18e, 18f, 19e and 19f were the most cytotoxic compounds against the tested cell lines. The results obtained in this work encourage further work in the future to produce new cytotoxic compounds.   KEY WORDS: Diphenylpropamide, 3-Phenylisoxazole, Pyran, Pyridine, Cytotoxicity Bull. Chem. Soc. Ethiop. 2023, 37(1), 141-158.                                                              DOI:https://dx.doi.org/10.4314/bcse.v37i1.1

Exploring the Nature of the Antimicrobial Metabolites Produced by <i>Paenibacillus ehimensis</i> Soil Isolate MZ921932 Using a Metagenomic Nanopore Sequencing Coupled with LC-Mass Analysis

The continuous emergence of multidrug-resistant (MDR) pathogens poses a global threat to public health. Accordingly, global efforts are continuously conducted to find new approaches to infection control by rapidly discovering antibiotics, particularly those that retain activities against MDR pathogens. In this study, metagenomic nanopore sequence analysis coupled with spectroscopic methods has been conducted for rapid exploring of the various active metabolites produced by Paenibacillus ehimensis soil isolate. Preliminary soil screening resulted in selection of a Gram-positive isolate identified via 16S ribosomal RNA gene sequencing as Paenibacillus ehimensis MZ921932. The isolate showed a broad range of activity against MDR Gram-positive, Gram-negative, and Candida spp. A metagenomics sequence analysis of the soil sample harboring Paenibacillus ehimensis isolate MZ921932 (NCBI GenBank accession PRJNA785410) revealed the presence of conserved biosynthetic gene clusters of petrobactin, tridecaptin, locillomycin (β-lactone), polymyxin, and macrobrevin (polyketides). The liquid chromatography/mass (LC/MS) analysis of the Paenibacillus ehimensis metabolites confirmed the presence of petrobactin, locillomycin, and macrobrevin. In conclusion, Paenibacillus ehimensis isolate MZ921932 is a promising rich source for broad spectrum antimicrobial metabolites. The metagenomic nanopore sequence analysis was a rapid, easy, and efficient method for the preliminary detection of the nature of the expected active metabolites. LC/MS spectral analysis was employed for further confirmation of the nature of the respective active metabolites

Position Paper on Global ECMO Education and Educational Agenda for the Future: A Statement from the Extracorporeal Life Support Organization ECMOed Taskforce

This is the author accepted version of the article at https://doi.org/10.1097/ccm.0000000000004158. Copyright © 2020 by the Society of Critical Care Medicine and Wolters Kluwer Health, Inc. All Rights Reserved.Objective The purpose of this position paper is two-fold: first, to describe the state of extracorporeal membrane oxygenation (ECMO) education worldwide, noting current limitations and challenges; and second, to put forth an educational agenda regarding opportunities for an international collaborative approach towards standardization. Design and Setting In 2018, the Extracorporeal Life Support Organization Education (ECMOed) Taskforce organized two structured, face-to-face meetings and conducted a review of published literature on ECMO education. Taskforce members generated a consensus statement using an iterative consensus process through teleconferences and electronic communication. Measurements and Main Results The ECMOed Taskforce identified seven educational domains that would benefit from international collaborative efforts. Of primary importance, the taskforce outlined actionable items regarding: (1) the creation of a standardized ECMO curriculum; (2) defining criteria for an ECMO course as a vehicle for delivering the curriculum; (3) outlining a mechanism for evaluating the quality of educational offerings; (4) utilizing validated assessment tools in the development of ECMO practitioner certification; and (5) promoting high-quality educational research to guide ongoing educational and competency assessment development. Conclusions Significant variability and limitations in global ECMO education exist. In this position paper, we outline a road map for standardizing international ECMO education and practitioner certification. Ongoing high-quality educational research is needed to evaluate the impact of these initiatives.Peer reviewe