267 research outputs found
Development of a technology for expression of recombinant human erythropoietin in cultured mammalian cells using alphavirus expression system
Currently there are no industrial eukaryotic expression systems other than transient
expression from plasmids or expression from genes integrated into host genome. Both approaches (use
of eukaryotic plasmids or chromosomal integration) suffer from poor scalability and often from poor
yields. Although, in laboratory settings, effective means for transducing of cultured cells to express
foreign proteins and for high-level transient expression were developed based on viral genomes. We
thought to develop a scalable and suitable for industrial application technology for the production of
recombinant human erythropoietin (EPO) in mammalian cell cultures using an expression vector based
on the genome of RNA virus
Development of a technology for expression of recombinant human erythropoietin in cultured mammalian cells using alphavirus expression system
Currently there are no industrial eukaryotic expression systems other than transient
expression from plasmids or expression from genes integrated into host genome. Both approaches (use
of eukaryotic plasmids or chromosomal integration) suffer from poor scalability and often from poor
yields. Although, in laboratory settings, effective means for transducing of cultured cells to express
foreign proteins and for high-level transient expression were developed based on viral genomes. We
thought to develop a scalable and suitable for industrial application technology for the production of
recombinant human erythropoietin (EPO) in mammalian cell cultures using an expression vector based
on the genome of RNA virus
Split core technology allows efficient production of virus-like particles presenting a receptor-contacting epitope of human IgE
Immunoglobulin E (IgE) plays a central role in type I hypersensitivity including allergy
and asthma. Novel treatment strategy envisages development of a therapeutic vaccine designed to elicit
autologous blocking antibodies against the IgE. We sought to develop an IgE-epitope antigen that induces
antibodies against a receptor-contacting epitope on human IgE molecule
Split core technology allows efficient production of virus-like particles presenting a receptor-contacting epitope of human IgE
Immunoglobulin E (IgE) plays a central role in type I hypersensitivity including allergy
and asthma. Novel treatment strategy envisages development of a therapeutic vaccine designed to elicit
autologous blocking antibodies against the IgE. We sought to develop an IgE-epitope antigen that induces
antibodies against a receptor-contacting epitope on human IgE molecule
Development of a recombinant foot -and-mouth disease vaccine
Foot-and-mouth disease (FMD) is important disease of cloven-foot animals including
cows and swine. Although annual vaccination against the FMD is mandated in regions of South
Kazakhstan, outbreaks of the disease are registered every year. These outbreaks result in huge economic
losses because international rules require culling of all the diseased and contacted animals and all
products from these animals must be destroyed. Currently available anti-FMD vaccines are all produced
using the original technology of inactivation of virus (foot-and-mouth disease virus, FMDV) grown in
cell cultures. Recombinant anti-FMD vaccine is a long anticipated development in the industry because
the recombinant vaccine is safe and compatible with diagnostic tests for discrimination of diseased and
vaccinated animals (DIVA)
Development of a recombinant foot -and-mouth disease vaccine
Foot-and-mouth disease (FMD) is important disease of cloven-foot animals including
cows and swine. Although annual vaccination against the FMD is mandated in regions of South
Kazakhstan, outbreaks of the disease are registered every year. These outbreaks result in huge economic
losses because international rules require culling of all the diseased and contacted animals and all
products from these animals must be destroyed. Currently available anti-FMD vaccines are all produced
using the original technology of inactivation of virus (foot-and-mouth disease virus, FMDV) grown in
cell cultures. Recombinant anti-FMD vaccine is a long anticipated development in the industry because
the recombinant vaccine is safe and compatible with diagnostic tests for discrimination of diseased and
vaccinated animals (DIVA)
The interaction of dark matter cusp with the baryon component in disk galaxies
In this paper we examine the effect of the formation and evolution of the
disk galaxy on the distribution of dark halo matter. We have made simulations
of isolated dark matter (DM) halo and two component (DM + baryons). N-body
technique was used for stellar and DM particles and TVD MUSCL scheme for
gas-dynamic simulations. The simulations include the processes of star
formation, stellar feedback, heating and cooling of the interstellar medium.
The results of numerical experiments with high spatial resolution let us to
conclude in two main findings. First, accounting of star formation and
supernova feedback resolves the so-called problem of cusp in distribution of
dark matter predicted by cosmological simulations. Second, the interaction of
dark matter with dynamic substructures of stellar and gaseous galactic disk
(e.g., spiral waves, bar) has an impact on the shape of the dark halo. In
particular, the in-plane distribution of dark matter is more symmetric in runs,
where the baryonic component was taken into account.Comment: 7 pages, 6 figure
The relationship of fracture toughness coefficients and geophysical characteristics of rocks of hydrocarbon deposits
This paper contains the results of laboratory tests to determine the fracture toughness coefficient KIC of rocks for terrigenous and carbonate objects by three methods. The tests were carried out by different methods due to the lack of a standard method for determining the fracture toughness characteristics of rocks in Russia. We used the following methods for determining the KIC coefficient: the extension of core specimens with an annular fracture, the action of a concentrated load on a beam specimen with a fracture and the method of bending semi-circular samples with a fracture according to ISRM recommendations.
The paper presents the relationship of the fracture toughness coefficients with the P-wave velocity and porosity. The obtained dependencies characterize the general trend of changing for the studied parameter and can be used in the design of hydraulic fracturing in the fields for which tests were conducted
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