MANAGEMENT EFFICIENCY OF STATE AND MUNICIPAL PROPERTY AT THE LEVEL OF A CONSTITUENT ENTITY OF THE RUSSIAN FEDERATION

The issues of state property management have been considered in the study, key problems in the management of state and municipal property have been presented, measures to eliminate them have been announced. It has been revealed that the current management system is not effective and requires management decisions aimed at achieving state goals. It also has been specified that there is a weak control over the processes of managing state property and the distribution of income. Methodological approaches to assessing the effectiveness of using state and municipal property have been reviewed. The opinion that it is advisable to use the principle of a balanced scorecard has been expressed. The results of scientific research will be useful for further theoretical developments on issues of public administration theory as a whole. The applied significance of the research results is due to the prospects of their application in the process of developing state policy on managing state property

Biochemical blood parameters and level of endogenous intoxication in cows suffering from hepatopathies under heat stress

Global warming results in increased extreme weather  events, including heatwaves,  droughts and floods, which exceed plants’and animals’tolerance thresholds, thus posing a threat to the economy and agriculture. Under these conditions, heat stress becomes a vital problem for animal husbandry. The paper presents the study results of biochemical blood parameters and endogenous intoxication in cows suffering from hepatopathies  under heat stress. Based on the calculated temperature-humidity  index, it was established that during the summer season in the conditions of the Krasnodar  Krai lowlands, cows are under heat stress. Using the method of paired comparisons, two groups of animals (n = 10) were formed: the first group was a healthy livestock; and the second group consisted of animals suffering from hepatic pathologies.  Blood was sampled from all cows at the beginning of the experiment (the first decade of May) and at the end (the last decade of July). Laboratory tests of blood revealed that as the heat stress develops healthy cows show the increase in the protein concentration in blood, and, on the contrary, animals with hepatic pathologies demonstrate the inhibition of protein synthesis. The higher activity of aminotransferases and alkaline phosphatase in the bovine serum in the summer season when compared to the spring season was established. The study of the endogenous intoxication level dynamics in cattle during the development of heat stress, showed that in both groups the concentrations of middle molecules (ММ) increased relative to the background data: in the first group (healthy cows) MM 237 – by 11.8%,  MM 254 – by 14.4%,  MM 280 – by 16.9%; in the second group (cattle with liver pathology)  MM 237 – by 16.9%,  MM 254 – by 20.3%,  MM 280 – by 33%. Thus, under heat stress, the endogenous intoxication in healthy livestock was almost 1.5 times less intense as compared to the animals suffering from hepatopathies

Yield formation of PVY-resistant and susceptible potato breeding lines

We studied the resistance to potato virus Y (PVY) in a potato hybrid population, and its effect on the yield formation under contrasting meteorological conditions. The virus resistance analysis was conducted after growing the plants under natural virus infection and after PVY inoculation. The dominant Ryadg gene allele in the potato breeding lines genotypes was revealed by detection of the PCR-based molecular marker RYSC3. Virus resistant lines were selected more often in the potato breeding program than susceptible forms due to higher productivity and larger number of tubers. PVY-resistant samples demonstrated a higher yield than susceptible forms. Significant differences (p < 0.05) were detected by univariate ANOVA in three of the four years studied. The virus resistance effect value ranged from 4 to 22%. Resistant form yield was more stable than that of susceptible one, which was evident from the corresponding coefficients of variation. Resistant samples also had a greater number of tubers per plant. At the same time, univariate ANOVA revealed significant differences (p < 0.05) only in one of the four studied years. The virus resistance effect ranged from 2 to 22%

Effect of scanning strategy on mechanical properties of Ti-6Al-4V alloy manufactured by laser direct energy deposition

In this work, we investigated the effect of DED thermal history on the obtained structural and mechanical properties of Ti-6Al-4V using a thermocouple. As a result of the study, the specific DED process parameters allow the same mechanical characteristics for as-built titanium alloy and the alloy after heat treatmen

Phase and structural changes during heat treatment of additive manufactured CrFeCoNi high-entropy alloy

In the current study, the effect of a post-printing heat treatment at 400-1000 ℃ for 24 h and for 21 days on the changes in structures and phase compositions of an AM CrFeCoNi alloy prepared by the laser powder bed fusion AM technique is presented to better understand a heat treatment-microstructure-property relationship of the AM HE

CLINICALLY RELEVANT MINOR HISTOCOMPATIBILITY ANTIGENS FOR RUSSIAN PATIENTS UNDERGOING HEMATOPOIETIC STEM CELL TRANSPLANTATION

Hematopoietic stem cell transplantation (HSCT) from healthy donors is used for blood cancer treatment. Alloreactive graft-versus-host disease (GvHD) is one of the post-transplant detrimental side effects, and the main reason for GVHD after HSCT fully matched for human leukocyte peptide antigens (HLA) presented by HLA molecules on cell surface. These polymorphic peptides, minor histocompatibility antigens (MiHA), arise from any genes, including those expressed at hematopoietic tissues. The latter may lead to the s.c. graft-versus-leukemia effect (GvL), thus preventing relapse of a malignancy. A*02:01 is one of the most frequent HLA alleles for European part of Russia. We assessed frequencies for 20 MiHA-encoded genetic polymorphisms, presented via A*02:01 allele, for plausible bone marrow donors, or hematopoietic stem cells (HSC) from the Donor Registry at Russian National Research Center for Hematology, we have also determined a number of immunogenic mismatches for these 20 MiHA in real donor – recipient pairs. A total of 608 potential donors, 90 donors and 92 recipients were genotyped. Using public data, we have shown that frequencies for MiHA coding genes are most close to appropriate frequencies among the European population. We have calculated probability of MiHA-specific alloimmune response after HSCT: there are chances of 33 and 75% for three or more immunogenic mismatches (IM) for related and unrelated HSCTs, respectively. Real frequencies for immune mismatch in 20 related and 20 unrelated donor – recipient pairs are in accordance with estimated theoretical probabilities. As based on the calculated frequencies, we suggest the LB-NDC80- 1P/A, LB-CCL4- 1T, and HA-1 MiHA to be the most promising minor antigens for targeted cell therapies of hematopoietic tissue malignancies. The data obtained could be used for planning allo-HSCTs in Russian patients

Test system for the detection of binding antibodies to interferon beta-1a for clinical use

Test system for determining the presence of binding antibodies to interferon beta-1a (BAB to IFN beta-1a) in serum using ELISA has been developed. The validation included sensitivity, selectivity, specificity, precision and accuracy, robustness, stability, suitability of the test system studies. The applicability of the mentioned test system has been approved for the use in clinical studies for the evaluation of interferon beta-1a immunogenicity (pegylated and non-pegylated forms)

Yield formation of PVY-resistant and susceptible potato breeding lines

We studied the resistance to potato virus Y (PVY) in a potato hybrid population, and its effect on the yield formation under contrasting meteorological conditions. The virus resistance analysis was conducted after growing the plants under natural virus infection and after PVY inoculation. The dominant Ryadg gene allele in the potato breeding lines genotypes was revealed by detection of the PCR-based molecular marker RYSC3. Virus resistant lines were selected more often in the potato breeding program than susceptible forms due to higher productivity and larger number of tubers. PVY-resistant samples demonstrated a higher yield than susceptible forms. Significant differences (p < 0.05) were detected by univariate ANOVA in three of the four years studied. The virus resistance effect value ranged from 4 to 22%. Resistant form yield was more stable than that of susceptible one, which was evident from the corresponding coefficients of variation. Resistant samples also had a greater number of tubers per plant. At the same time, univariate ANOVA revealed significant differences (p < 0.05) only in one of the four studied years. The virus resistance effect ranged from 2 to 22%