25 research outputs found
The effect of humic substances on test-objects
The complex study of a number of humic substances was conducted with help of bioassay methods using invertebrates (Daphnia, paramecium, earthworms), terrestrial (wheat seeds) and aquatic plants (elodea, duckweed) and algae (Scenedesmus quadricauda). The ranges of concentrations of humates that have a negative and a stimulating effect on biological objects were identified. The efficiency of biological testing methods to evaluate the effect of humic substances on biological objects is shown
ΠΠ΅ΡΠ°Π»ΡΠ½ΠΎΠ΅ ΠΈΠ·ΡΡΠ΅Π½ΠΈΠ΅ ΠΎΠ΄Π½ΠΎΠΉ ΠΈΠ· ΡΠΎΠ»ΡΠ½ΠΎΠΊΡΠΏΠΎΠ»ΡΠ½ΡΡ ΡΡΡΡΠΊΡΡΡ ΠΠΌΠ±ΠΈΠ½ΡΠΊΠΎΠ³ΠΎ ΡΠ°ΠΉΠΎΠ½Π° ΠΡΠΈΠΊΠ°ΡΠΏΠΈΠΉΡΠΊΠΎΠΉ Π²ΠΏΠ°Π΄ΠΈΠ½Ρ Π³Π΅ΠΎΡΠΈΠ·ΠΈΡΠ΅ΡΠΊΠΈΠΌΠΈ ΠΌΠ΅ΡΠΎΠ΄Π°ΠΌΠΈ (Π Π΅ΡΠΏΡΠ±Π»ΠΈΠΊΠ° ΠΠ°Π·Π°Ρ ΡΡΠ°Π½)
ΠΠ΅ΡΠ°Π»ΡΠ½ΠΎΠ΅ ΠΈΠ·ΡΡΠ΅Π½ΠΈΠ΅ ΠΎΠ΄Π½ΠΎΠΉ ΠΈΠ· ΡΠΎΠ»ΡΠ½ΠΎΠΊΡΠΏΠΎΠ»ΡΠ½ΡΡ
ΡΡΡΡΠΊΡΡΡ ΠΠΌΠ±ΠΈΠ½ΡΠΊΠΎΠ³ΠΎ ΡΠ°ΠΉΠΎΠ½Π° ΠΡΠΈΠΊΠ°ΡΠΏΠΈΠΉΡΠΊΠΎΠΉ Π²ΠΏΠ°Π΄ΠΈΠ½Ρ Π³Π΅ΠΎΡΠΈΠ·ΠΈΡΠ΅ΡΠΊΠΈΠΌΠΈ ΠΌΠ΅ΡΠΎΠ΄Π°ΠΌΠΈ (Π Π΅ΡΠΏΡΠ±Π»ΠΈΠΊΠ° ΠΠ°Π·Π°Ρ
ΡΡΠ°Π½).Detailed study of one of the salt dome structures of the Emba region of the Caspian basin (Republic of Kazakhstan) by geophysical methods)
ΠΠ½ΡΠ΅Π½ΡΠΈΡΠΈΠΊΠ°ΡΠΈΡ ΠΎΡΠΈΡΡΠΊΠΈ ΡΡΠΎΡΠ½ΠΎΠΉ Π²ΠΎΠ΄Ρ ΠΎΡ Π½Π΅ΡΡΠ΅ΠΏΡΠΎΠ΄ΡΠΊΡΠΎΠ² Ρ ΠΏΠΎΠΌΠΎΡΡΡ ΠΊΠ°ΡΠ°Π»ΠΈΠ·Π°ΡΠΎΡΠΎΠ² ΡΠΈΠΏΠ° Β«ΠΠ°ΡΠ°Π½Β» ΠΈ ΡΡΠ°ΠΌΠΌΠΎΠ² Π±ΠΈΠΎΠ΄Π΅ΡΡΡΡΠΊΡΠΎΡΠΎΠ² Π½Π΅ΡΡΠΈ
The treatment wastewater with a high concentration of oil at development of the oil refining industry is issue of the day. The treatment of wastewater enhanced to combination of chemical and biological purification methods. Treatment of wastewater using strains of biodegraders oil that it mixed with the activated sludge and the use of the catalysts βKatanβ reduces the amount oil in waste water in the model by more than 80%.ΠΡΠΈ ΡΠ°Π·Π²ΠΈΡΠΈΠΈ Π½Π΅ΡΡΠ΅ΠΏΠ΅ΡΠ΅ΡΠ°Π±Π°ΡΡΠ²Π°ΡΡΠ΅ΠΉ ΠΏΡΠΎΠΌΡΡΠ»Π΅Π½Π½ΠΎΡΡΠΈ ΠΏΡΠΎΠ±Π»Π΅ΠΌΠ° ΠΎΡΠΈΡΡΠΊΠΈ ΡΡΠΎΡΠ½ΡΡ
Π²ΠΎΠ΄ Ρ Π²ΡΡΠΎΠΊΠΎΠΉ ΠΊΠΎΠ½ΡΠ΅Π½ΡΡΠ°ΡΠΈΠ΅ΠΉ Π½Π΅ΡΡΠΈ ΡΠ²Π»ΡΠ΅ΡΡΡ Π΄ΠΎΡΡΠ°ΡΠΎΡΠ½ΠΎ Π°ΠΊΡΡΠ°Π»ΡΠ½ΠΎΠΉ. Π‘ΠΎΡΠ΅ΡΠ°Π½ΠΈΠ΅ Ρ
ΠΈΠΌΠΈΡΠ΅ΡΠΊΠΈΡ
ΠΈ Π±ΠΈΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈΡ
ΠΌΠ΅ΡΠΎΠ΄ΠΎΠ² ΠΎΡΠΈΡΡΠΊΠΈ ΠΏΠΎΠ²ΡΡΠ°Π΅Ρ ΡΡΡΠ΅ΠΊΡΠΈΠ²Π½ΠΎΡΡΡ ΠΎΡΠΈΡΡΠΊΠΈ ΡΡΠΎΡΠ½ΡΡ
Π²ΠΎΠ΄. ΠΠΎΠ²ΡΡΠ΅Π½ΠΈΠ΅ Π΄ΠΎΠ»ΠΈ Π½Π΅ΡΡΠ΅ΡΠ°Π·ΡΡΡΠ°ΡΡΠΈΡ
ΠΌΠΈΠΊΡΠΎΠΎΡΠ³Π°Π½ΠΈΠ·ΠΌΠΎΠ² Π² Π°ΠΊΡΠΈΠ²Π½ΠΎΠΌ ΠΈΠ»Π΅ ΠΈ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΠ΅ ΠΊΠ°ΡΠ°Π»ΠΈΠ·Π°ΡΠΎΡΠΎΠ² ΡΠΈΠΏΠ° Β«ΠΠ°ΡΠ°Π½Β» ΠΏΠΎΠ·Π²ΠΎΠ»ΡΠ΅Ρ ΡΠΌΠ΅Π½ΡΡΠΈΡΡ ΠΊΠΎΠ»ΠΈΡΠ΅ΡΡΠ²ΠΎ Π½Π΅ΡΡΠ΅ΠΏΠ΅ΡΠΎΠ΄ΡΠΊΡΠΎΠ² Π² ΠΌΠΎΠ΄Π΅Π»ΡΠ½ΠΎΠΉ ΡΡΠΎΡΠ½ΠΎΠΉ Π²ΠΎΠ΄Π΅ Π±ΠΎΠ»Π΅Π΅ ΡΠ΅ΠΌ Π½Π° 80%
BIOLOGICAL ACTIVITY OF CARBONIC NANO-STRUCTURES-COMPARISON VIA ENZYMATIC BIOASSAY
Purpose: The aim of the work is to compare the biological activity of carbonic nano-structures of natural and artificial origination, namely, humic substances (HS) and fullerenols. Materials and methods: The representative of the fullerenol group, Π‘ 60 Π y (OH) x where Ρ + x = 20β22, was chosen. Enzyme-based luminescent bioassay was applied to evaluate toxicity and antioxidant properties of HS and fullerenol (F); chemiluminescent luminol method was used to study a content of reactive oxygen species (ROS) in the solutions. Toxicity of the bioactive compounds was evaluated using effective concentrations ΠΠ‘ 50 ; detoxification coefficients D OxT were applied to study and compare antioxidant activity of the compounds. Antioxidant activity and ranges of active concentrations of the bioactive compounds were determined in model solutions of organic and inorganic oxidizersβ1,4-benzoquinone and potassium ferricianide. Results and discussion: Values of ΠΠ‘ 50 revealed higher toxicity of HS than F (0.005 and 0.108 g L β1 , respectively); detoxifying concentrations of F were found to be lower. Antioxidant ability of HS was demonstrated to be time-dependent; the 50-min preliminary incubation in oxidizer solutions was suggested as optimal for the detoxification procedure. On the contrary, Fβ antioxidant effect demonstrated independency on time. Antioxidant effect of HS did not depend on amphiphilic characteristics of the media (values of D OxT were 1.3 in the solutions of organic and inorganic oxidizers), while this of F was found to depend: it was maximal (D OxT = 2.0) in solutions of organic oxidizer, 1,4-benzoquinone. Conclusions: Both HS and F demonstrated toxicity and low-concentration antioxidant ability; however, quantitative characteristics of their effects were different. The differences were explained with HS polyfunctionality, higher ability to decrease ROS content, non-rigidity, and diffusion restrictions in their solutions. Antioxidant effect of the bioactive compounds was presumably attributed to catalytic redox activity of their Ο-fragments. The paper demonstrates a high potential of luminescent enzymatic bioassay to study biological activity of nano-structures of natural and artificial origination
Comparative assessment of toxic effects of surfactants using biotesting methods
Π’Π΅ΠΊΡΡ ΡΡΠ°ΡΡΠΈ Π½Π΅ ΠΏΡΠ±Π»ΠΈΠΊΡΠ΅ΡΡΡ Π² ΠΎΡΠΊΡΡΡΠΎΠΌ Π΄ΠΎΡΡΡΠΏΠ΅ Π² ΡΠΎΠΎΡΠ²Π΅ΡΡΡΠ²ΠΈΠΈ Ρ ΠΏΠΎΠ»ΠΈΡΠΈΠΊΠΎΠΉ ΠΆΡΡΠ½Π°Π»Π°.This study assesses the comparative sensitivity and possibility of obtaining fast results of various methods of biotesting for several surfactants: Tween 85, sodium dodecyl sulfate (SDS), Fairy dishwashing gel, and Mif washing powder. The following test organisms are used for the study: luminescent bacteria Photobacterium phosphoreum (Beijerinck), preparation of Ecolum luminescent bacteria, unicellular algae Scenedesmus quadricauda (G.M. Smith), infusorian Paramecium caudatum (Ehrenberg), and crustacean Daphnia magna (Straus). It has been revealed that Fairy dishwashing gel possesses the strongest toxicity against the studied test objects. Daphnia and algae are most sensitive to the effects of Fairy and SDS, protozoan and luminescent bacteria are most sensitive to SDS, and Ecolum is most sensitive to Mif washing powder. The tested aquatic organisms and Ecolum are most tolerant to the effect of Tween 85
Comparative assessment of toxic effects of surfactants using biotesting methods
Π’Π΅ΠΊΡΡ ΡΡΠ°ΡΡΠΈ Π½Π΅ ΠΏΡΠ±Π»ΠΈΠΊΡΠ΅ΡΡΡ Π² ΠΎΡΠΊΡΡΡΠΎΠΌ Π΄ΠΎΡΡΡΠΏΠ΅ Π² ΡΠΎΠΎΡΠ²Π΅ΡΡΡΠ²ΠΈΠΈ Ρ ΠΏΠΎΠ»ΠΈΡΠΈΠΊΠΎΠΉ ΠΆΡΡΠ½Π°Π»Π°.This study assesses the comparative sensitivity and possibility of obtaining fast results of various methods of biotesting for several surfactants: Tween 85, sodium dodecyl sulfate (SDS), Fairy dishwashing gel, and Mif washing powder. The following test organisms are used for the study: luminescent bacteria Photobacterium phosphoreum (Beijerinck), preparation of Ecolum luminescent bacteria, unicellular algae Scenedesmus quadricauda (G.M. Smith), infusorian Paramecium caudatum (Ehrenberg), and crustacean Daphnia magna (Straus). It has been revealed that Fairy dishwashing gel possesses the strongest toxicity against the studied test objects. Daphnia and algae are most sensitive to the effects of Fairy and SDS, protozoan and luminescent bacteria are most sensitive to SDS, and Ecolum is most sensitive to Mif washing powder. The tested aquatic organisms and Ecolum are most tolerant to the effect of Tween 85