Reconversion of Cytochrome P420 into P450 and Reactivation of Hydroxylases in Microsomal Membranes Reconstituted by Self-Assembly

Membrane vesicles formed by means of dialysis of microsomal proteins and lipids, solubilized by cholate, contained an equal quantity of cytochromes P420 and P450. Addition of albumin and phosphatidylcholine aids reconversion of cytochrome P420 into cytochrome P450. In the presence of albumin the reconstituted membranes contained less lysophosphatidylcholine than those reconstituted without it. Comparative analysis does not reveal· marked differences in the phospholipid and protein composition or specific activity and content of NAD(P)H-dependent redox carriers of original and reconstituted membranes. In contrast to original membranes, the reconstituted ones do not possess aniline hydroxylase activity. The NADH-specific dimethylaniline demethylase activity was higher than that of the original membranes. NADPH-dependent demethylase of this substrate could be reconstituted to 50-800/o of its initial activity. The reconstituted membranes differ from original membranes by their higher non-enzymatic lipid peroxidase activity. Thus, it appears that dialysis in the presence of albumin and phosphatidylcholine is the most effective mode of microsomal membrane reconstitution

Social hate: the features of modern extremist

The article presents some specific characteristics of political extremist’s personality. The authors present that having deep psychological clashes extremists very often transfer it to the external world and design the destabilization of social and political life of separate countries and regions. As a result they provoke the conflicts and hostility in the relationships between state power and the people.В докладе раскрываются некоторые особенности личности политического экстремиста. Показано, что испытывая глубокие психологические коллизии, экстремисты очень часто переносят их во внешний мир, проектируя дестабилизацию общественно-политической жизни отдельных стран и регионов. И вносят конфликты и вражду во взаимоотношения государственной власти с населением

Laboratory biomarker galectin-3 in the diagnostics of myocardial inflammatory changes in patients with atrial fibrillation

Atrial fibrillation (AF) is one of the most common cardiac arrhythmias. Numerous data indicate a significant contribution of myocardial inflammatory changes in the development and progression of AF. The search for new laboratory biomarkers to assess the activity of myocardial inflammatory processes, and the study of their diagnostic significance for noninvasive diagnosis in patients with AF is relevant. Therefore, the aim was to study the features of the serum level of the biomarker Gal-3 and to identify its relationship with inflammatory changes in the myocardium in patients with AF. Depending on the results of histological studies, the patients were divided into 2 groups: group 1 – with morphologically verified active lymphocytic myocarditis (ALM), group 2 – with lymphocytic infiltration (LI). Analysis of the frequency of detection and severity of the inflammatory process in the myocardium showed that activity of 4-5 scores was detected only in group 1. In 2nd group, activity of the inflammatory process in most patients was 1 score. All patients with LI mild interstitial inflammation were showed. In the ALM group moderate and severe interstitial inflammation was detected. A high number of CD3+ and CD45+ cells were found in 1st group compared to group 2 (p < 0.001).There were no significant intergroup differences in the serum level of Gal-3. At the same time, in 1st group showed a significant decrease in Gal-3 in 6 months after treatment (p = 0.028). Positive correlations of Gal-3 with the severity of the inflammatory process and endocardial involvement were revealed in patients with ALM. The association of serum Gal-3 levels with CD68+ levels in 1st group was detected (R = 0.48, p = 0.030). In 2nd group, a correlation between the level of Gal-3 in 6 months after ablation with infiltration of CD45+ cells was found (R = 0.69, p = 0.003). Thus, in patients with AF and active lymphocytic myocarditis, significant associations were established between biomarkers of Gal-3 and inflammatory changes in the myocardium. This confirms the important role of Gal-3 as a participant in the inflammatory process

Reconversion of Cytochrome P420 into P450 and Reactivation of Hydroxylases in Microsomal Membranes Reconstituted by Self-Assembly

Membrane vesicles formed by means of dialysis of microsomal proteins and lipids, solubilized by cholate, contained an equal quantity of cytochromes P420 and P450. Addition of albumin and phosphatidylcholine aids reconversion of cytochrome P420 into cytochrome P450. In the presence of albumin the reconstituted membranes contained less lysophosphatidylcholine than those reconstituted without it. Comparative analysis does not reveal· marked differences in the phospholipid and protein composition or specific activity and content of NAD(P)H-dependent redox carriers of original and reconstituted membranes. In contrast to original membranes, the reconstituted ones do not possess aniline hydroxylase activity. The NADH-specific dimethylaniline demethylase activity was higher than that of the original membranes. NADPH-dependent demethylase of this substrate could be reconstituted to 50-800/o of its initial activity. The reconstituted membranes differ from original membranes by their higher non-enzymatic lipid peroxidase activity. Thus, it appears that dialysis in the presence of albumin and phosphatidylcholine is the most effective mode of microsomal membrane reconstitution

The limit of mass determination with an AFM cantilever-based system

This study was performed in the framework of the Program for Basic Research of State Academies of Sciences for 2013-2020. Shumov I.D. is a recipient of Russian Federation President scholarship for young scientists for 2016-2018 (project identificator SP-4280.2016.4)

Magnetron sputtering deposition of ultra-thin tungsten coatings onto amorphous graphite for enhancement of horseradish peroxidase adsorption

The present study was performed in the framework of the Program for Basic Research of State Academies of Sciences for 2013-2020

Antithrombotic Therapy in Patients with Paroxysmal Atrial Fibrillation after Catheter Treatment

Aim. To study the efficacy and safety of antithrombotic therapy in patients with paroxysmal atrial fibrillation (AF) after catheter treatment during 36 months of follow-up.Material and methods. The retrospective observational study included 592 patients (283 men) who underwent catheter treatment of AF, aged 26 to 86 years (median age was 61.0 [55; 67]) with paroxysmal AF, treated in cardiac arrhythmias department of the Institute of Cardiology of Tomsk National Research Medical Center from 01.01.2017 to 31.12.2019. All patients were retrospectively divided into 2 groups: the first group consisted of patients with effective AF ablation, the second - with ineffective AF ablation. During follow-up after 12, 24 and 36 months, patients' complaints, documented arrhythmia recurrences, adherence to the prescribed treatment, and adverse clinical events were taken into account.Results. In patients with paroxysmal AF, the effectiveness of catheter treatment was 73.1% after 12 months of follow-up, 69.3% – after 24 months, 71.6% – after 36 months. The analysis of our data showed that during the follow-up period of 36 months, the incidence of ischemic stroke against the background of anticoagulant therapy and effective catheter treatment of paroxysmal AF was significantly lower than in patients with unsuccessful ablation (0.3% (n=1) and 3.7% (n=4), respectively), even despite the fact that not all patients from the first group received prescribed medication.Conclusion. The use of anticoagulant therapy in patients with paroxysmal AF after interventional treatment is safe, since the invasive strategy in combination with anticoagulant therapy does not increase the risk of major and minor bleeding, and in the case of effective intervention allows statistically significantly reduce the risk of ischemic stroke and almost completely eliminate the likelihood of other thromboemolic complications

Effect of catheter ablation for atrial fibrillation on left and right atrial function

Aim. To evaluate the effect of catheter ablation on left (LA) and right atria (RA) function in patients with atrial fibrillation.Material and methods. The study included 28 patients (14 men and 14 women) aged 33 to 72 years (mean age, 57,7±9,9 years) with paroxysmal (n=23) and persistent AF (n=5). All patients underwent radiofrequency ablation (RFA) with pulmonary vein antrum isolation. Before ablation and 3 days after, transthoracic twodimensional echocardiography was performed in sinus rhythm with an assessment of LA reservoir, conduit and booster pump function and RA peak longitudinal strain.Results. In the studied patients, a significant decrease in the reservoir, conduit and booster pump function of the LA was revealed after RFA, while there was no significant change in RA peak longitudinal strain after catheter ablation. LA reservoir, conduit and booster pump function decreased by 6,45% (p<0,001), 3,59% (p<0,001), 2,85% (p<0,001), respectively, while RA peak longitudinal strain increased by 0,73% (p=0,43).Conclusion. Catheter ablation has a significant damaging effect on the LA tissue, inhibiting the reservoir, pumping and pipeline functions. At the same time, the contractility of the PP in the early postoperative period improves, but not significantly