Experimental Solid Medium for Legionella Cultivation

Presented is the principal scheme for preparation of experimental medium for Legionella cultivation on the basis of fermentative hydrolysate of pig lung. Shown is that the culture medium has good growth properties and meets the requirements of regulatory documentation for culture medium used for isolation and cultivation of the causative agent of legionellosis

Quality Assessment of the Live Plague Vaccine Prepared Using nutrient Medium on the Basis of Hydrolysate of Concentrated Corn steep

Objective of the study was to test the nutrient medium based on the enzymatic hydrolysate of corn extract condensed for a scaled production of live plague vaccine and to check the quality of the obtained batches against the specified parameters. Materials and methods .A dense nutrient medium based on corn extract was used to grow biomass in the process of live plague vaccine production. The quality parameters of the vaccine preparation obtained were studied by the regulated methods set forth in the regulatory documentation. Results and conclusions. The vaccine was monitored at all stages of its manufacture, including control of the finished dosage form, in strict accordance with the approved regulatory documentation. All the experimental production series complied with the specified indices. Approbation of the production cycle environment for live plague vaccine manufacturing showed efficiency of the conditions and the possibility of environment’s application in the industrial production of the preparation

Quality Management System in Stavropol Research Anti-Plague Institute

The analysis of the structure of quality management system and the areas of its activity is presented. Trends for its further improvement are defined

Validation of Technological Process of Production of Liquid Brucellosis Diagnosticum for Agglutination Reaction, Suspension for Diagnostic Purposes

Presented are the results of validation of technological process of production of brucellosis diagnosticu

Construction and Approval of the Test-System for the Detection of Antibodies to Anthrax Agent Using Indirect Fluorescent Immunoassay

Constructed and validated has been the test-system for the detection of antibodies to anthrax agent using indirect fluorescent immunoassay. The panel consists of antigen preparation, positive control sample, negative control, and FITS-labeled rabbit antibodies. Studied have been 10 acapsular B. anthracis strains with different plasmid content and colony morphology. B. anthracis vaccine strain STI-1 is demonstrated to be the optimal one as antigen preparation. Sensitivity of this test-system is not less than 1:40, the working dilution of FITC-conjugate being 1:16. Specificity of the panel has been studied using 100 sera of healthy donors. Approved is 100 % reproducibility of the technique at different time intervals, as well as when carried by different specialists. The test-system allows for confirmation of anthrax diagnosis in humans

Matrix-represented constraints satisfaction methods: practical aspects of their implementation

The paper proposes an original approach to solving the problem of ineffective processing of qualitative constraints of a subject domain in the framework of constraint programming technology. The approach is based on the use of specialized matrix-like structures, providing a "compressed" representation of constraints over finite domains, as well as using author’s infer- ence algorithms on these structures. Compared to the prototypes using the typical representation of multi-place relations in a form of tables, the techniques make it possible to more efficiently reduce the search space. The paper presents practical aspects of implementation of user-developed types of constraints and corresponding algorithms-propagators with the help of constraint programming libraries. The algorithms performance has been assessed to clearly demonstrate the advantages of representation and processing of qualitative constraints of a subject domain by means of the above matrix structures

Qualitative modeling of technical systems based on the constraint propagation methods

В статье описан разработанный автором метод распространения ограничений, предназначенный для решения задач качественного моделирования технических систем. В отличие от аналогов, использующих для описания ограничений над конечными доменами структуры наподобие реляционных таблиц, предлагаемый метод опирается на применение оригинальных матрицеподобных структур, которые позволяют “сжато” представлять нечисловые зависимости между параметрами модели и эффективно их обрабатывать. The constraint propagation technique developed by the author is presented in the article. The technique is developed for tasks of qualitative modeling of technical systems. Despite of prototypes that use such structures as relational tables for constraints representation over finite domains, the proposed technique relies on using of original matrix-like structures allowing represent qualitative regularities between parameters of the model in compressed form and speed up their processing

Qualitative modeling of technical systems based on the constraint propagation methods

В статье описан разработанный автором метод распространения ограничений, предназначенный для решения задач качественного моделирования технических систем. В отличие от аналогов, использующих для описания ограничений над конечными доменами структуры наподобие реляционных таблиц, предлагаемый метод опирается на применение оригинальных матрицеподобных структур, которые позволяют “сжато” представлять нечисловые зависимости между параметрами модели и эффективно их обрабатывать. The constraint propagation technique developed by the author is presented in the article. The technique is developed for tasks of qualitative modeling of technical systems. Despite of prototypes that use such structures as relational tables for constraints representation over finite domains, the proposed technique relies on using of original matrix-like structures allowing represent qualitative regularities between parameters of the model in compressed form and speed up their processing

THE DRIVING FORCES OF RUSSIA’S PIVOT TO EAST

There is no conceptual analysis of the Russia’s pivot to East while a lot of discussion about nature and consequences of this geopolitical maneuver among both Russian and foreign analysts. The article based on the analysis of Russian official program documents explores the key aspects of the Russia`s reorientation to Asia policy. Authors view the policy in the context of three global processes: 1) participation of Russia in global competition towards reconfiguration of global order; 2) Russian strategy for reintegration of post-Soviet space and its interaction with alternatives projects for post-Soviet integration; 3) diversification of political and economic ties of Russia with its West/East partners. According to the authors’ conclusions, the ‘pivot to East’ process is unfolding in several dimensions simultaneously. Current geopolitical reconfiguration is the result of long-term processes which closely related with main geopolitical and internal priorities of Moscow, and this gives the reasons for vision of the pivot to East as sustainable rising tendency, which will independent from current and short-run processes in the international affairs. The factors, which directly led Russia to this policy, were rapprochement with China, building of cooperation and integration infrastructure with Asia-Pacific and post-Soviet Central Asia countries, diversification of energy resources trade with the purposes of avoid dependency from monopsonic European market

SEASONAL CHANGES OF VERTICAL PROFILES OF CHLOROPHYLL A IN THE AMUR BAY (JAPAN SEA)

Seasonal changes of chlorophyll a profiles are traced over the Amur Bay (Peter the Great Bay, Japan Sea) in May-October, 2017 by means of oceanographic sonde-profiler equipped with fluorometer. Two principally different types of the vertical profiles are revealed, which were formed by different mechanisms of productivity: i) Chl a concentration had the maximum at the sea surface and decreased with the depth in the internal part of the bay occupied by the estuarine waters, and ii) Chl a concentration had the maximum below the seasonal pycnocline in the external part of the bay connected with the open sea. The highest Chl a concentration was observed in July-August for the estuarine type because of summer monsoon flood on the rivers, but in September for the marine type because of the coastal upwelling induced by monsoon winds change. Comparing these results with estimations of Chl a concentration made with the satellite data, insufficient correspondence is concluded for the external part of the bay, outside the estuarine zone, because the satellite data don’t reflect well the chlorophyll a in the subsurface layer and its seasonal variations. Thus, underestimation of real productivity and feeding ability of marine areas is available with the satellite data on chlorophyll a