DYNAMIC OF CILIARY BODY MORPHOLOGICAL CHANGES AFTER PRESERVING CYCLODESTRUCTION DURING EARLY EXPERIMENTAL PERIOD

We've investigate ultrastructural manifestations of cryoapplicator made from a porous and permeable titan nikelid post-traumatic influence on ciliary body tissues in a rabbit's eye wall. During the experiment on 25 adult rabbits 3, 7, 14 and 30 days after local cryoinfluence eye wall fragments taken from experimental and control animals passed the corresponding procedures for electron microscopy. Already received factogrammes primary viewing convinces in specificity of cryoinfluence effect and structural stromal and a ciliary body elements' remodeling. Thus pinpoint morphological analysis of perinecrotic and remote zones of a stroma and ciliary body cryoapplication testifies more expressive their structural integrity, in comparison with usual mechanical damage consequences by the end of the first supervision week. In a peritraumatic wound zone ultrastructural manifestations of all the myoid and fibrous ciliary body elements intracellular regeneration and reorganization, fibroblasts to miofibroblasts transdifferentiation are stated along with signs of its scarring on the 2-nd and 3-rd weeks of experiment

Level of local cytokine production in the event of nickelid titanium suture material use in experimental surgical hypotension eye operation

Comparative assessment of cytokine moisture in aqueous chamber with experimental rabbits while use of nickelid titanium suture and in case of traditional suture material in hypotension operations. During the experiment, moisture in aqueous chamber has been studied with 10 adult rabbits, the samples were taken on 3rd, 7th and 30th days and in 3 months after hypotension surgery administered with nickelid titanium suture used as a retention suture. Control group consisted of 5 rabbits, for whom hypotension surgery has been performed with traditional suture material and of 6 intact rabbits. Amount of cytokines in biological fluid (aqueous chamber moisture - ACM) of the animals has been investigated in with enzyme immunodetection method to identify content of cytokines (IL-1β, TNF-α, IL-2, IL-10). The adverse nature of cytokines balance change and quotient of its pro - and anti-inflammatory components in the dynamics after intraocular implantation with TH-10 suture, that indicates positive anti-inflammatory influence of retention suture of nickelid titanium and becomes evident in significant reduction of anti-inflammatory component in cytokine balance, that develops in the farthermost monitoring terms, as well as in clinically unresponsive post-operative course with animals of test group compared to comparison group

LEVEL OF LOCAL PRODUCTION OF CYTOKINES IN THE CLINIC OF TRADITIONAL TREATMENT OF DEMODECTIC BLEPHARITIS AND IN CONDITIONS OF LID CRYOTHERAPY USE

Aim. A comparative cytokine profile assessment of the lacrimal fluid of the eyes of patients in the clinic of traditional treatment of demodectic blepharitis, and using the course of cryotherapy of the eyelids by an autonomous cryoapplicator made of titanium nickelide. Materials and methods. Clinical studies were conducted in 65 patients with demodectic blepharoconjunctivitis (DBC). A tear of the eye was taken on the 3rd, 7th and 30th days after the traditional treatment, and using cryotherapy of the eyelids by an autonomous cryoapplicator made of porous permeable titanium nickelide cooled in liquid nitrogen. In the studied biological fluid (tear) of patients with DBC, the content of cytokines (IL1β, IL8, IFNα, IL2, IL10) was evaluated by an enzyme immunoassay. Results. The opposite nature of the changes in the balance of cytokines and the ratio of its pro and anti-inflammatory links in the time of observation was revealed. A significant dynamic increase of IFNa and IL2 content in the lacrimal fluid of the examined after cryostimulation can evidence for an adequate activation of cellular immune link under its interaction with Demodex mite antigens, as well as for reinforcing the regeneration mechanisms. Conclusion. The analysis of obtained data evidences for the high efficiency of clinical and immunological effect of lid cryotherapy method for DBC treatment