REVERSE LOGISTICS IN THE RUSSIAN ORGANIZATIONS: THEORETICAL AND PRACTICAL ASPECTS

The arguments proving necessity of development of the logistic management concept by material, information, financial and returnable flows are provided. The problems of the Russian logistics connected with its efficiency, the quality level and questions of processing (utilization), correction of defective products (works, services) - returnable (reverse logistics), or logistics of returnable flows are considered. The most typical stages of management of reverse flows are allocated. Problems of reverse logistics of organizational and methodical character are systematized. Actions for the solution of the designated problems are offered

Educational ecosystem in conditions of digital transformation

Currently, there is an active digital transformation of management practices and processes in the world, associated with the emergence of an ecosystem approach. The ecosystem approach is being actively implemented in all spheres of the economy and society. This phenomenon has not bypassed the education sphere. The article considers the priority tasks and trends in the development of education that meet the global challenges of the changing world economy, and aimed at developing a new paradigm of training throughout life. The purpose of the study is to analyze the factors influencing the creation of an educational ecosystem of the future, designed to form and develop a culture of lifelong learning, and, consequently, to the need for the introduction of network models based on elements of joint and voluntary learning. The research methods are comparative and causal analysis of management decisions. As a result of the study, the authors identify the elements of the educational and personnel ecosystems that affect the business processes of an organization in the context of digital transformation. It will significantly accelerate the possibilities of cooperation between the real sector of the economy, the educational ecosystem and the state within the framework of the above changes. The ecosystem transformation of education will allow to educate a new generation that will lead the transition to the effective and harmonious development of world civilization. The ecosystem approach will change the traditional ways of learning, discovering, acting on the principles of cooperation. These changes are aimed at dialogue in terms of business training in the conditions of digitalisation of the economy between the educational ecosystem and the business environment. Therefore, it is the personnel service for business processes that should be given increased attention to representatives of the real sector of the economy, the scientific community and educational institutions

REVERSE LOGISTICS IN THE RUSSIAN ORGANIZATIONS: THEORETICAL AND PRACTICAL ASPECTS

The arguments proving necessity of development of the logistic management concept by material, information, financial and returnable flows are provided. The problems of the Russian logistics connected with its efficiency, the quality level and questions of processing (utilization), correction of defective products (works, services) - returnable (reverse logistics), or logistics of returnable flows are considered. The most typical stages of management of reverse flows are allocated. Problems of reverse logistics of organizational and methodical character are systematized. Actions for the solution of the designated problems are offered

QUALITY MANAGEMENT SYSTEM OF EDUCATIONAL PROGRAMS IN BUSINESS SCHOOL

The arguments substantiating the necessity of creation and development of a quality management system in educational programs in business schools are presented. The problems of evaluating the quality of educational programs related to their effectiveness are examined. The problems of internal control of educational programs, monitoring the results of training and the quality of teaching are systematized. Special attention is paid to the solution of organizational and methodological problems: the formation of a system of analytical indicators that allows to effectively implement the main objectives of assessing the quality of education, the study and self-assessment of the state of development and efficiency of the business school

Introduction of mixed forms of education based on the digital and analog kinds integration of the organization of the educational process of universities

The abrupt transition to the distance learning format during the COVID-19 pandemic showed that the legal framework for regulating the introduction of new digital telecommunications technologies is largely lagging behind in educational organizations, and in some areas, in particular, related to the regulation of “remote” labor relations in higher education is completely absent. The lack of proper legal regulation leads not only to labor conflicts, but also to legal proceedings between students and universities. The article presents an incomplete list of documents (without disclosing their contents) that need to be developed or modified. The study proposes a roadmap that takes into account the parallel work with the constant coordination of the contents of the documents between the working groups. The authors conclude that trade union committees, student and teaching communities should take an active part in the development of documentation, since the proposed transformation of the educational process affects all participants of the educational process in universities without exception

Optimisation of the Schizosaccharomyces pombe urg1 expression system

The ability to study protein function in vivo often relies on systems that regulate the presence and absence of the protein of interest. Two limitations for previously described transcriptional control systems that are used to regulate protein expression in fission yeast are: the time taken for inducing conditions to initiate transcription and the ability to achieve very low basal transcription in the "OFF-state". In previous work, we described a Cre recombination-mediated system that allows the rapid and efficient regulation of any gene of interest by the urg1 promoter, which has a dynamic range of approximately 75-fold and which is induced within 30-60 minutes of uracil addition. In this report we describe easy-to-use and versatile modules that can be exploited to significantly tune down P urg1 "OFF-levels" while maintaining an equivalent dynamic range. We also provide plasmids and tools for combining P urg1 transcriptional control with the auxin degron tag to help maintain a null-like phenotype. We demonstrate the utility of this system by improved regulation of HO-dependent site-specific DSB formation, by the regulation Rtf1-dependent replication fork arrest and by controlling Rhp18(Rad18)-dependent post replication repair

Protease Activated Receptor Signaling Is Required for African Trypanosome Traversal of Human Brain Microvascular Endothelial Cells

Human African trypanosomiasis, or sleeping sickness, occurs when single-cell trypanosome protozoan parasites spread from the blood to brain over the blood-brain barrier (BBB). This barrier is composed of brain microvascular endothelial cells (BMECs) especially designed to keep pathogens out. Safe drugs for treating sleeping sickness are lacking and alternative treatments are urgently required. Using our human BMEC BBB model, we previously found that a parasite protease, brucipain, induced calcium activation signals that allowed this barrier to open up to parasite crossing. Because human BMECs express protease-activated receptors (PARs) that trigger calcium signals in BMECs, we hypothesized a functional link between parasite brucipain and BMEC PARs. Utilizing RNA interference to block the production of one type of PAR called PAR-2, we hindered the ability of trypanosomes to both open up and cross human BMECs. Using gene-profiling methods to interrogate candidate BMEC pathways specifically triggered by brucipain, several pathways that potentially link brain inflammatory processes were identified, a finding congruent with the known role of PAR-2 as a mediator of inflammation. Overall, our data support a role for brucipain and BMEC PARs in trypanosome BBB transmigration, and as potential triggers for brain inflammation associated with the disease

Enhancement of immune response of HBsAg loaded poly(L-lactic acid) microspheres against Hepatitis B through incorporation of alum and chitosan

Purpose: Poly (L-lactic acid) (PLA) microparticles encapsulating Hepatitis B surface antigen (HBsAg) with alum and chitosan were investigated for their potential as a vaccine delivery system. Methods: The microparticles, prepared using a water-in-oil-in-water (w/o/w) double emulsion solvent evaporation method with polyvinyl alcohol (PVA) or chitosan as the external phase stabilising agent showed a significant increase in the encapsulation efficiency of the antigen. Results: PLA-Alum and PLA-chitosan microparticles induced HBsAg serum specific IgG antibody responses significantly higher than PLA only microparticles and free antigen following subcutaneous administration. Chitosan not only imparted a positive charge to the surface of the microparticles but was also able to increase the serum specific IgG antibody responses significantly. Conclusions: The cytokine assays showed that the serum IgG antibody response induced is different according to the formulation, indicated by the differential levels of interleukin 4 (IL-4), interleukin 6 (IL-6) and interferon gamma (IFN-γ). The microparticles eliciting the highest IgG antibody response did not necessarily elicit the highest levels of the cytokines IL-4, IL-6 and IFN-γ

Optical biosensor differentiates signaling of endogenous PAR1 and PAR2 in A431 cells

<p>Abstract</p> <p>Background</p> <p>Protease activated receptors (PARs) consist of a family of four G protein-coupled receptors. Many types of cells express several PARs, whose physiological significance is mostly unknown.</p> <p>Results</p> <p>Here, we show that non-invasive resonant waveguide grating (RWG) biosensor differentiates signaling of endogenous protease activated receptor subtype 1 (PAR₁) and 2 (PAR₂) in human epidermoid carcinoma A431 cells. The biosensor directly measures dynamic mass redistribution (DMR) resulted from ligand-induced receptor activation in adherent cells. In A431, both PAR₁and PAR₂agonists, but neither PAR₃nor PAR₄agonists, trigger dose-dependent Ca²⁺mobilization as well as G_q-type DMR signals. Both Ca²⁺flux and DMR signals display comparable desensitization patterns upon repeated stimulation with different combinations of agonists. However, PAR₁and PAR₂exhibit distinct kinetics of receptor re-sensitization. Furthermore, both trypsin- and thrombin-induced Ca²⁺flux signals show almost identical dependence on cell surface cholesterol level, but their corresponding DMR signals present different sensitivities.</p> <p>Conclusion</p> <p>Optical biosensor provides an alternative readout for examining receptor activation under physiologically relevant conditions, and differentiates the signaling of endogenous PAR₁and PAR₂in A431.</p

CD44 Expression in Oro-Pharyngeal Carcinoma Tissues and Cell Lines

Expression of CD44, a transmembrane hyaluronan-binding glycoprotein, is variably considered to have prognostic significance for different cancers, including oral squamous cell carcinoma. Although unclear at present, tissue-specific expression of particular isoforms of CD44 might underlie the different outcomes in currently available studies. We mined public transcriptomics databases for gene expression data on CD44, and analyzed normal, immortalized and tumour-derived human cell lines for splice variants of CD44 at both the transcript and protein levels. Bioinformatics readouts, from a total of more than 15,000 analyses, implied an increased CD44 expression in head and neck cancer, including increased expression levels relative to many normal and tumor tissue types. Also, meta-analysis of over 260 cell lines and over 4,000 tissue specimens of diverse origins indicated lower CD44 expression levels in cell lines compared to tissue. With minor exceptions, reverse transcribed polymerase chain reaction identified expression of the four main isoforms of CD44 in normal oral keratinocytes, transformed lines termed DT and HaCaT, and a series of paired primary and metastasis-derived cell lines from oral or pharyngeal carcinomas termed HN4/HN12, HN22/HN8 and HN30/HN31. Immunocytochemistry, Western blotting and flow cytometric assessments all confirmed the isoform expression pattern at the protein level. Overall, bioinformatic processing of large numbers of global gene expression analyses demonstrated elevated CD44 expression in head and neck cancer relative to other cancer types, and that the application of standard cell culture protocols might decrease CD44 expression. Additionally, the results show that the many variant CD44 exons are not fundamentally deregulated in a diverse range of cultured normal and transformed keratinocyte lines