Contribution of midgut bacteria to blood digestion and egg production in aedes aegypti (diptera: culicidae) (L.)

<p>Abstract</p> <p>Background</p> <p>The insect gut harbors a variety of microorganisms that probably exceed the number of cells in insects themselves. These microorganisms can live and multiply in the insect, contributing to digestion, nutrition, and development of their host.</p> <p>Recent studies have shown that midgut bacteria appear to strengthen the mosquito's immune system and indirectly enhance protection from invading pathogens. Nevertheless, the physiological significance of these bacteria for mosquitoes has not been established to date. In this study, oral administration of antibiotics was employed in order to examine the contribution of gut bacteria to blood digestion and fecundity in <it>Aedes aegypti</it>.</p> <p>Results</p> <p>The antibiotics carbenicillin, tetracycline, spectinomycin, gentamycin and kanamycin, were individually offered to female mosquitoes. Treatment of female mosquitoes with antibiotics affected the lysis of red blood cells (RBCs), retarded the digestion of blood proteins and reduced egg production. In addition, antibiotics did not affect the survival of mosquitoes. Mosquito fertility was restored in the second gonotrophic cycle after suspension of the antibiotic treatment, showing that the negative effects of antibiotics in blood digestion and egg production in the first gonotrophic cycle were reversible.</p> <p>Conclusions</p> <p>The reduction of bacteria affected RBC lysis, subsequently retarded protein digestion, deprived mosquito from essential nutrients and, finally, oocyte maturation was affected, resulting in the production of fewer viable eggs. These results indicate that <it>Ae. aegypti </it>and its midgut bacteria work in synergism to digest a blood meal.</p> <p>Our findings open new possibilities to investigate <it>Ae. aegypti</it>-associated bacteria as targets for mosquito control strategies.</p

RFX machine and power supply improvements for RFP advanced studies

Experimental results and theoretical studies call for Reversed Field Experiment (RFX) machine and power supply improvements to allow studies that go beyond those of a conventional Reversed Field Pinch (RFP) with passively stabilized turbulent MHD dynamo. The new paths opened by recent results in RFX and other RFP machines are introduced; then the goals and the design lines of the technical modifications of RFX, mainly addressed to improve the first wall, the plasma magnetic boundaries and to increase the operational flexibility of the toroidal field circuit power supply, are reported

Complete Characterization of Phase and Amplitude of Bichromatic Extreme Ultraviolet Light

Intense, mutually coherent beams of multiharmonic extreme ultraviolet light can now be created using seeded free-electron lasers, and the phase difference between harmonics can be tuned with attosecond accuracy. However, the absolute value of the phase is generally not determined. We present a method for determining precisely the absolute phase relationship of a fundamental wavelength and its second harmonic, as well as the amplitude ratio. Only a few easily calculated theoretical parameters are required in addition to the experimental data