14 research outputs found
Impact of oxidative stress on Acanthamoeba castellanii mitochondrial bioenergetics depends on cell growth stage
Hydroxynonenal, a lipid peroxidation end product, stimulates uncoupling protein activity in Acanthamoeba castellanii mitochondria; the sensitivity of the inducible activity to purine nucleotides depends on the membranous ubiquinone redox state
Effect of temperature on fatty acid metabolism in skeletal muscle mitochondria of untrained and endurance-trained rats - Fig 2
<p><b>Determination of protein levels in skeletal muscle homogenates (A) and mitochondria (B) from control (</b>c<b>) and trained (t) rats. A</b>, Representative Western blots and analyses of the protein expression of PGC1α, mitochondrial marker (Mito marker), Nrf2, CD36, β-actin and GADPH. <b>B</b>, Representative Western blots and analyses of the protein expression of ACADS, CPT1A, CD36, COXII, (Mito marker). Expression levels were normalized for β actin or GADPH (<b>A</b>, <i>right panel</i>) and mito marker or COXII (<b>B</b>, <i>right panel</i>). The data (± SD, <i>n</i> = 6) is from six independent homogenate or mitochondrial preparations from six different animals from each group. ***<i>P</i> < 0.001, **<i>P</i> < 0.01, *<i>P</i> < 0.05 vs. value obtained for control rats.</p
Effect of temperature on fatty acid metabolism in skeletal muscle mitochondria of untrained and endurance-trained rats - Fig 1
<p><b>The influence of endurance training on mitochondrial oxidation of palmitoylcarnitine (A) and glycerol-3-phosphate (B) at 25</b>°<b>C, 35</b>°<b>C and 42</b>°<b>C.</b> Respiratory rates were measured in mitochondria isolated from control (c) and trained (<b>t</b>) rats in the presence of 150 μM ADP (state 3, phosphorylating respiration) with 0.5 mM palmitoyl-DL-carnitine (<b>A</b>) or 5 mM glycerol-3-phosphate (<b>B</b>). Mean values (± SD) for six mitochondria preparations from six different animals from each group (<i>n</i> = 6) are shown. <sup>###</sup><i>P</i> < 0.001, <sup>##</sup><i>P</i> < 0.01, <sup>#</sup><i>P</i> < 0.05 vs value obtained at 35°C for a given group of animals, i.e., within control rats or trained rats. ***<i>P</i> < 0.001, **<i>P</i> < 0.01, *<i>P</i> < 0.05 vs. value obtained for control rats for a given assay temperature.</p
Respiratory rates and coupling parameters in control and trained rat skeletal muscle mitochondria at 25°C, 35°C and 42°C.
<p>Respiratory rates and coupling parameters in control and trained rat skeletal muscle mitochondria at 25°C, 35°C and 42°C.</p