22 research outputs found
416 DOSE TITRATION FOR SUPERSTIMULATION OF BRANGUS AND BONSMARA DONORS WITH Folltropin®-V BY A SINGLE INTRAMUSCULAR INJECTION
No full text291 SUPEROVULATORY RESPONSE IN BEEF CATTLE TREATED DURING THE FIRST FOLLICULAR WAVE FOLLOWING SYNCHRONIZATION OF OVULATION WITH A PROGESTIN DEVICE AND GnRH
No full text404 SUPEROVULATORY RESPONSE IN BEEF DONORS TREATED DURING THE FIRST FOLLICULAR WAVE OR FOUR DAYS AFTER PROGESTERONE AND ESTRADIOL ADMINISTRATION
No full text420 SUPERSTIMULATION OF ANGUS DONORS WITH A SINGLE INTRAMUSCULAR INJECTION OF FOLLTROPIN®-V
No full text230 SUPEROVULATORY RESPONSE OF BEEF COWS WITH OR WITHOUT A CORPUS LUTEUM AT THE TIME OF INSERTION OF A PROGESTERONE RELEASING DEVICE
No full text207 OOCYTE RECOVERY RATES AND IN VITRO BLASTOCYST PRODUCTION IN CATTLE TREATED WITH A SINGLE INJECTION OF FOLLTROPIN-V DILUTED IN A SLOW-RELEASE FORMULATION
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Dickkopf-related protein 1 is a progestomedin acting on the bovine embryo during the morula-to-blastocyst transition to program trophoblast elongation
No full textDevelopment of a suitable manufacturing process for production of a bioactive recombinant equine chorionic gonadotropin (reCG) in CHO-K1 cells
No full textEquine chorionic gonadotropin (eCG) is a heterodimeric glycoprotein hormone produced by pregnant mares that has been used to improve reproductive performance in different domestic species. Several strategies to produce the hormone in a recombinant way have been reported; nevertheless, no approach has been able to produce a recombinant eCG (reCG) with significant in vivo bioactivity or in sufficient quantities for commercial purposes. For this reason, the only current product available on the market consists of partially purified preparations from serum of pregnant mares (PMSG). Herein, we describe a highly efficient process based on third-generation lentiviral vectors as delivery method for the production of reCG in suspension CHO-K1 cells, with productivities above 20 IU 106 cell−1.d−1 and 70% purification yields after one purification step. Importantly, reCG demonstrated biological activity in cattle, since around 30 μg of reCG were needed to exert the same biologic effect of 400 IU of PMSG in an ovulation synchronization protocol. The results obtained demonstrate that the developed strategy represents an attractive option for the production of reCG and constitutes an auspicious alternative for the replacement of animals as a source of PMSG.Fil: Villarraza, Carlos Javier. Universidad Nacional del Litoral; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; ArgentinaFil: Antuña, Sebastián. No especifíca;Fil: Tardivo, María Belén. No especifíca;Fil: Rodríguez, María Celeste. Universidad Nacional del Litoral; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; ArgentinaFil: Mussio, Pablo Esteban. Universidad Nacional del Litoral; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; ArgentinaFil: Cattaneo, Luciano. Universidad Nacional del Litoral; ArgentinaFil: Fontana, Diego Sebastian. Universidad Nacional del Litoral; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; ArgentinaFil: Díaz, Pablo Uriel. Universidad Nacional del Litoral; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; ArgentinaFil: Ortega, Hugo Hector. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; ArgentinaFil: Tríbulo, Andres. Instituto de Reproducción Animal Córdoba; ArgentinaFil: Macagno, Alejandro. Universidad Nacional de Villa María; ArgentinaFil: Bó, Gabriel A.. Universidad Nacional de Villa María; Argentina. Instituto de Reproducción Animal Córdoba; ArgentinaFil: Ceaglio, Natalia Analia. Universidad Nacional del Litoral; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; ArgentinaFil: Prieto, Claudio. Universidad Nacional del Litoral; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentin
Effects of sex on response of the bovine preimplantation embryo to insulin-like growth factor 1, activin A, and WNT7A
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