37 research outputs found
Cell adhesion molecule DM-GRASP presented in different nanopatterns to neurons regulates attachment and neurite growth
Adhesion and neurite formation of neurons and neuroblastoma cells critically depends on the lateral spacing of the cell adhesion molecule DM-GRASP offered as nanostructured substrate
High-affinity chelator thiols for switchable and oriented immobilization of histidine-tagged proteins: A generic platform for protein chip technologies
Oriented binding of the His(6)-tagged carboxyl-tail of the L-type Ca2+ channel alpha(1)-subunit to a new NTA-functionalized self-assembled monolayer
Synthesis of a multivalent chelator lipid for stably tethering histidine-tagged proteins onto membranes
Synthesis of a multivalent chelator lipid for stably tethering histidine-tagged proteins onto membranes
Intravesicular and intervesicular interaction by orthogonal multivalent host–guest and metal–ligand complexation
Host vesicles composed of amphiphilic β-cyclodextrin CD1 recognize metal-coordination complexes of the adamantyl-functionalized ethylenediamine ligand L via hydrophobic inclusion in the β-cyclodextrin cavities at the vesicle surface. In the case of Cu(II) and L, the resulting coordination complex was exclusively CuL(2), and the interaction with the host vesicles was intravesicular, unless the concentration of metal complex and vesicles was high (>0.1 mM). In the case of Ni(II) and L, a mixture was formed consisting of mainly NiL and NiL(2), the interaction with the host vesicles was effectively intervesicular, and addition of the guest–metal complex resulted in aggregation of the vesicles into dense, multilamellar clusters even in dilute solution [1 μM Ni(II)]. The metal–L complex could be eliminated by a strong chelator such as EDTA, and the intervesicular interaction could be suppressed by a competitor such as unmodified β-cyclodextrin. The result from this investigation is that the strongest metal-coordination complex [Cu(II) with L] binds exclusively intravesicularly, whereas the weakest metal-coordination complex [Ni(II) with L] binds predominantly intervesicularly and is the strongest interfacial binder. These experimental observations are confirmed by a thermodynamic model that describes multivalent orthogonal interactions at interfaces