Formen der Verdrossenheit und Kritik an Wissenschaft und Technik in der Gegenwartskultur. Bd 1-3 Bd 1: Situationsdiagnose und exemplarische Literaturanalyse. Empirische Befunde und Hinweise zur Einstellung in der Bevoelkerung. Bd 2: Exploration der Alternativkultur: Die Wissenschafts- und Technikkritik der Alternativbewegung. Bd 3: Bibliographie

SIGLETIB: AC 7792 / FIZ - Fachinformationszzentrum Karlsruhe / TIB - Technische InformationsbibliothekDEGerman

Microsatellite markers in the Australian desert plant, Solanum centrale (Solanaceae)

Premise of the study: The first microsatellite primers were developed for Solanum centrale, a native Australian species used in the bush foods industry. Existing markers developed for other Solanum species were also amplified.\ud \ud Methods and Results: Using an enrichment cloning protocol, seven novel markers were developed, and 48 existing markers from other Solanum species were tested, resulting in the characterization of a set of six highly polymorphic co-dominant loci for use in S. centrale. Microsatellite screening revealed polyploidy. Among the six highly polymorphic loci, allelic diversity ranged from 7 to 14.\ud \ud Conclusions: These markers will be useful for investigating genetic diversity and as a simple way of estimating ploidy of wild populations

Evaluation of androgenic competence through anther culture in common eggplant and related species

Anther culture is a convenient technique to obtain androgenic haploid and doubled haploid (DH) plants. In common eggplant (Solanum melongena), this technique has been used to develop DH pure lines for producing uniform F1 hybrid seed of some commercial varieties. However, a comprehensive study of the variation of this useful trait among different materials of common eggplant and related species is still lacking. In this work, we studied the androgenic response of 12 accessions of common eggplant and related materials from the primary (eggplant complex) and secondary genepools. We cultured anthers of all the accessions under the same experimental conditions, and studied their competence to produce calli, embryos and plants, as well as the quality and origin of the embryos produced. In our conditions, anthers of 11 out of the 12 accessions produced somatic calli, whereas only 5 also produced microspore-derived embryos, with variable results in terms of embryo quality and of frequency of embryo induction and plant germination. Embryos of responding accessions were initially haploid, and reached the DH status, verified with SSR markers, after a defined period of culture. In addition to other aspects common to many androgenesis-responsive species, our results allowed us to extract conclusions particular to common eggplant and relatives, including the difficulty for finding sources of androgenic competence out of S. melongena, the reduced impact of calli in the production of non-DH individuals, and the need to avoid the occurrence of severe anatomical and functional problems in the apex of most embryos, which seriously reduces their germinative success. © 2011 Springer Science+Business Media B.V.We acknowledge Drs. Begona Renau and Antonio Serrano, and Mrs Nuria Palacios for their excellent technical work, as well as the staff of the COMAV greenhouses for their valuable help. Thanks are also due to Dr. Santiago Vilanova for providing us with the SSRs used in this work. This work was supported by grants from Spanish MICINN AGL2006-06678 and AGL2010-17895 to JMSS, and AGL2009-07257 to JP.Salas Aragon, P.; Prohens Tomás, J.; Seguí-Simarro, JM. (2011). Evaluation of androgenic competence through anther culture in common eggplant and related species. Euphytica. 182(2):261-274. doi:10.1007/s10681-011-0490-2S261274182