Promoting Fundamental movement skill development and physical activity in early childhood settings : a cluster randomized controlled trial

The aim of this study was to assess the feasibility, acceptability and potential efficacy of a physical activity program for preschool children. A 20-week, 2-arm parallel cluster randomized controlled pilot trial was conducted. The intervention comprised structured activities for children and professional development for staff. The control group participated in usual care activities, which included designated inside and outside playtime. Primary outcomes were movement skill development and objectively measured physical activity. At follow-up, compared with children in the control group, children in the intervention group showed greater improvements in movement skill proficiency, with this improvement statically significant for overall movement skill development (adjust diff. = 2.08, 95% CI 0.76, 3.40; Cohen&rsquo;s d = 0.47) and significantly greater increases in objectively measured physical activity (counts per minute) during the preschool day (adjust diff. = 110.5, 95% CI 33.6, 187.3; Cohen&rsquo;s d = 0.46). This study demonstrates that a physical activity program implemented by staff within a preschool setting is feasible, acceptable and potentially efficacious.<br /

Flow around individual Taylor bubbles rising in stagnant CMC solutions: PIV measurements

The flow around single Taylor bubbles rising in non-Newtonian solutions of Carboxymethylcellulose (CMC) polymer was studiedusing a simultaneous technique employing particle image velocimetry (PIV) and shadowgraphy. This technique solved previousproblems on finding the correct position of the bubble interface. Solutions of different polymer weight percentage, varying from0.1 to 1.0 wt% were used to cover a wide range of flow regimes. The rheological fluid properties and pipe dimension yieldedReynolds numbers between 4 and 254 and Deborah numbers between 0.012 and 0.402. The shape of the bubbles in the differentfluids was compared. The flow around the nose of the bubbles was found to be similar in all the studied conditions. Velocityprofiles in the liquid film around the bubble were measured. Different wake structures were found in the different solutionsstudied. With increasing viscosity, the wake flow varied from turbulent to laminar, being possible to observe a negative wake forthe higher polymer concentration solutions. A comparison between the different wake structures was made

Characterization of silver particles in the stratum corneum of healthy subjects and atopic dermatitis patients dermally exposed to a silver-containing garment

Silver is increasingly being used in garments to exploit its antibacterial properties. Information on the presence of silver nanoparticles (AgNPs) in garments and their in vivo penetration across healthy and impaired skin from use is limited. We investigated the presence of AgNPs in a silver containing garment and in the stratum corneum (SC) of healthy subjects (CTRLs) and individuals with atopic dermatitis (AD). Seven CTRLs and seven AD patients wore a silver sleeve (13% Ag w/w) 8 h/day for five days on a forearm and a placebo sleeve on the other forearm. After five days, the layers of the SC were collected by adhesive tapes. The silver particles in the garment and SC were characterized by scanning electron microscopy with energy dispersive X-ray analysis (SEM-EDX) and atomic force microscopy (AFM). AFM and SEM revealed the presence of sub-micrometre particles having a broad range of sizes (30\u2013500 nm) on the surface of the garment that were identified as silver. On the SC tapes collected from different depths, aggregates with a wide range of sizes (150 nm\u20132 \u3bcm) and morphologies were found. Most aggregates contained primarily silver, although some also contained chlorine and sulfur. There was no clear difference in the number or size of the aggregates observed in SC between healthy and AD subjects. After use, AgNPs and their aggregates were present in the SC at different depths of both healthy subjects and AD patients. Their micrometre size suggests that aggregation likely occurred in the SC

Simultaneous PIV and shadowgraphy measurements in slug flow in Newtonian and non-Newtonian liquids

A recent technique for performing simultaneous Particle Image Velocimetry and Shadography appliedfor the first time to slug flow, is presented in this work. The unsteadiness of slug flow creates the needof recording the shadow of the Taylor bubbles at the precise instant and position of the PIVmeasurements. Therefore, this experimental technique is used in order to characterise simultaneouslythe flow in the liquid and the shape of a single gas slug (Taylor bubble) rising through a verticalcolumn of stagnant liquid using only one CCD camera. The experimental facility and technique aredescribed and the details of the synchronisation between the two techniques are also explained. Thisnew method is based on the fact that the PIV particles and the shadow of the bubbles can illuminate theCCD camera sensor at different gray levels. The PIV images are obtained by seeding the flow withfluorescent seeding and placing an optical filter in front of the camera so that the intense laserreflections in the interface are avoided and only the PIV particles reach the CCD sensor. Theshadowgraph images are obtained by backward illumination with a board of LEDs emitting light in awavelength that passes through the optical filter. The processing of the images is explained. Thereason for keeping recording images with 8 bits is discussed. During the post-processing it is possibleto combine the shadowgraph results with the velocity field acquired with PIV, therefore, solving someof the basic processing errors, which appear at the interfaces. Some limitations of the technique itself,due to the highly 3D shape of the rear of the bubbles are also discussed. The flow around a gas slugrising through a vertical column filled with a non-Newtonian fluid is also presented. These are the firstquantitative measurements in slug flow for non-Newtonian flows, as long as the authors are awar

A genome-wide expression analysis identifies a network of EpCAM-induced cell cycle regulators

Expression of the epithelial cell adhesion molecule EpCAM is upregulated in a variety of carcinomas. This antigen is therefore explored in tumour diagnosis, and clinical trials have been initiated to examine EpCAM-based therapies. Notably, the possible intracellular effects and signalling pathways triggered by EpCAM-specific antibodies are unknown. Here, we show treatment of the mouse lung carcinoma cell line A2C12, of the human lung carcinoma cell line A549 and the human colorectal cell line Caco-2 with the monoclonal EpCAM antibody G8.8 to cause dose dependently an increase in cell proliferation, as determined by the MTS and the 5′-bromo-2′-deoxyuridine (BrdU) labelling assay. Furthermore, a genome-wide approach identified networks of regulated genes, most notably cell cycle regulators, upon treatment with an EpCAM-specific antibody. Indeed, changes in the expression of cell cycle regulators agreed well with the BrdU labelling data, and an analysis of differentially expressed genes revealed the processes with the strongest over-representation of modulated genes, for example, cell cycle, cell death, cellular growth and proliferation, and cancer. These data suggest that EpCAM is involved in signal transduction triggering several intracellular signalling pathways. Knowing EpCAM signalling pathways might lead to a reassessment of EpCAM-based therapies

Crop Updates - 2003 Oilseeds

This session covers fifteen papers from different authors ACKNOWLEDGMENTS VARIETIES Large scale canola varietal evaluation in WA, Peter Nelson, Oilseeds WA Performance of IT and TT canola varieties in the medium and high rainfall agzones of WA 2001-02, Graham Walton, Hasan Zaheer and Paul Carmody, Department of Agriculture QUALITY Reproductive biology, cotyledon development and oil accumulation in canola, J.A. Fortescue and D.W. Turner, School of Plant Biology, Faculty of Natural and Agricultural Sciences, The University of Western Australia Plant and environmental factors affecting oil concentration in canola – a mini-review, D.W. Turner, School of Plant Biology, Faculty of Natural and Agricultural Sciences, The University of Western Australia Potential benefits from interspecific crosses between canola and ‘near canola’ quality Indian mustard, Janet Wroth, School of Plant Biology, The University of Western Australia (UWA), Wallace Cowling, School of Plant Biology, UWA and CBWA Pty Ltd, Anh-Van Pham, School of Mathematics and Statistics, UWA NUTRITION, AGRONOMY AND MACHINERY Timing of nitrogen application for producing canola grain and oil, R. F. Brennan, Department of Agriculture Managing canola for soil type and moisture stress, Paul Carmody and Hasan Zaheer Department of Agriculture Machinery lessons from 2002 – canola establishment, Glen Riethmuller, Greg Hamilton and Jo Hawksley, Department of Agriculture Machinery lessons from 2002 – harvesting short crops, Glen Riethmuller, Department of Agriculture Does increasing canola seeding rate reduce the competitiveness of grass weeds? Zaicou-Kunesch, C.M., Zaheer, S.H. and Eksteen, D, Department of Agriculture PESTS AND DISEASES Aphid damage to canola – not all cultivars are equal, Françoise A. Berlandier and Christiaan Valentine, Department of Agriculture Should we be worried about developing insecticide resistance in aphids? Owain Edwards, CSIRO Entomology Benefits provided by treating canola seed with imidacloprid seed dressing, Roger Jones, Brenda Coutts, Lisa Smith and Jenny Hawkes, Department of Agriculture, and Centre for Legumes in Mediterranean Agriculture Blackleg levels in canola in 2002, Ravjit Khangura1, Moin Salam1, Art J Diggle1 and Martin J Barbetti1,2 1Department of Agriculture, 2University of Western Australia DBM in canola, Kevin Walden, Department of Agricultur

Crop Updates 2000 - Lupins

This session covers nineteen papers from different authors: 1.1999 Lupin Highlights, Bill O’Neill, LUPIN PRODUCTIVITY IMPROVEMENTS AND INDUSTRY DEVELOPMENT LUPIN ANTHRACNOSE 2. Anthracnose – 1999/2000, Geoff Thomas and Mark Sweetingham, Agriculture Western Australia LUPIN BREEDING AND AGRONOMY 3. The genetic control of mildly restricted branching in narrow-leafed lupin (Lupinus augustifolius L), Kedar Adhikari1,3, Nick Galwey1,3 and Miles Dracup2,3 1Plant Sciences, University of Western Australia 2Agriculture Western Australia 3Cooperative Research Centre for Legumes in Mediterranean Agriculture, University of Western Australia 4. Genotype x time of sowing interaction in lupins – Mingenew, Bob French, Agriculture Western Australia 5. Genotype x time of sowing interaction in lupins – Wongan Hills, Bob French, Agriculture Western Australia 6. Genetic variation in lupin tolerance to Brown Leaf Spot, Bob French, Agriculture Western Australia 7. Yellow lupin management in Western Australia, Bob French, Agriculture Western Australia APHIDS AND VIRUS CONTROL 8. Forecasting aphid and virus risk in lupins, Debbie Thackray, Jenny Hawkes and Roger Jones, Centre for Legumes in Mediterranean Agriculture and Agriculture Western Australia 9. When should lupin crops be sprayed for aphids to achieve maximum yield response? Françoise Berlandier, Agriculture Western Australia 10. Yield limiting potential of the new, non-necrotic strain of bean yellow mosaic virus in narrow-leafed lupin, Roger Jones, Yvonne Cheng and Lisa Smith, Crop Improvement Institute, Agriculture Western Australia, and Centre for Legumes in Mediterranean Agriculture LUPIN NUTRITION 11. Increasing the value of a rotation by applying lime, Chris Gazey and Michael O’Connell, Agriculture Western Australia HERBICIDE TOLERANCE AND WEED CONTROL 12. Herbicide damage does not mean lower yield in Lupins, Peter Carlton, Trials Coordinator, Elders Limited 13. Effect of herbicides Tordonä 75D and Lontrelä, used for eradication of Skeleton Weed, on production of Lupins in following seasons, John R. Peirce and Brad J. Rayner, Agriculture Western Australia 14. Herbicide tolerance of lupins, Terry Piper, Agriculture Western Australia 15. Tanjil lupins will tolerate metribuzin under the right conditions, Peter Newman, Agronomist Elders Limited and Cameron Weeks, Mingenew/Irwin Group LUPIN ESTABLISHMENT 16. A new seed pressing system for ryegrass suppression and healthy lupin establishment, Mohammad Amjad and Glen Riethmuller,Agriculture Western Australia 17. Banded surfactant for better lupin yield on non-wetting sand, Dr Paul Blackwell, Agriculture Western Australia DROUGHT TOLERANCE 18. Drought tolerance of lupin genotypes in Western Australia, Jairo A. Palta1,2,, Neil C. Turner1,2, Robert J. French2,3 ,1CSIRO Plant Industry, Centre for Mediterranean Agricultural Research, 2Centre for Legumes in Mediterranean Agriculture, University of Western Australia, 3Agriculture Western Australia, 19. Stem carbohydrate in lupins: a possible buffer to maintain seed growth under adverse conditions, Bob French1, Tim Setter2, Jairo Palta3 , 1Agriculture Western Australia, and CLIMA, 2Agriculture Western Australia, 3CSIRO, Floreat Park, and CLIM

A fully human anti-Ep-CAM scFv-beta-glucuronidase fusion protein for selective chemotherapy with a glucuronide prodrug

Monoclonal antibodies against tumour-associated antigens could be useful to deliver enzymes selectively to the site of a tumour for activation of a non-toxic prodrug. A completely human fusion protein may be advantageous for repeated administration, as host immune responses may be avoided. We have constructed a fusion protein consisting of a human single chain Fv antibody, C28, against the epithelial cell adhesion molecule and the human enzyme β-glucuronidase. The sequences encoding C28 and human enzyme β-glucuronidase were joined by a sequence encoding a flexible linker, and were preceded by the IgGκ signal sequence for secretion of the fusion protein. A CHO cell line was engineered to secrete C28-β-glucuronidase fusion protein. Antibody specificity and enzyme activity were retained in the secreted fusion protein that had an apparent molecular mass of 100 kDa under denaturing conditions. The fusion protein was able to convert a non-toxic prodrug of doxorubicin, N-[4-doxorubicin-N-carbonyl(oxymethyl)phenyl]-O-β-glucuronyl carbamate to doxorubicin, resulting in cytotoxicity. A bystander effect was demonstrated, as doxorubicin was detected in all cells after N-[4-doxorubicin-N-carbonyl(oxymethyl)phenyl]-O-β-glucuronyl carbamate administration when only 10% of the cells expressed the fusion protein. This is the first fully human and functional fusion protein consisting of an scFv against epithelial cell adhesion molecule and human enzyme β-glucuronidase for future use in tumour-specific activation of a non-toxic glucuronide prodrug

Crop Updates 2008 - Weeds

This session covers twenty nine papers from different authors: 1. BOXER® GOLD, a new pre-emergent herbicide option for WA wheat and barley growers for the control of Annual Ryegrass and Toad Rush, Craig A. Ruchs, Syngenta Crop Protection Australia Pty Ltd 2. Efficacy of Boxer Gold in the control of annual ryegrass in wheat, Dr Abul Hashem, Dr Catherine Borger, Department of Agriculture and Food, Mr Ken McKee, Field Development Manager, Syngenta Crop Protection Australia Pty Ltd 3. Alternative herbicides to avoid trifluralin resistance, Catherine Borger and Abul Hashem, Department of Agriculture and Food 4. Exiting new herbicides for ryegrass control in wheat, Peter Newman, Department of Agriculture and Food 5. Herbicide options for resistant wild radish in wheat, Peter Newman, Department of Agriculture and Food 6. A near-complete control of wild radish with three new herbicide products, Aik Cheam and Siew Lee, Department of Agriculture and Food 7. An investigation of diflufenican resistance mechanism/s in wild radish, Meagan Pearce, Dr Michael Walsh and Prof. Stephen Powles, Western Australian Herbicide Resistance Initiative, School of Plant Biology, University of WA 8. Synergistic effects of Group C and GroupF herbicides on resistant and susceptible wild radish populations, Kent Stone, Dr Michael Walsh and Prof. Stephen Powles, Western Australian Herbicide Resistance Initiative, School of Plant Biology, University of WA 9. Precept® for the management of wild radish resistant to PDS inhibiting herbicides, Mike Clarke and Andrew Loorham, Bayer Cropscience Pty Ltd, Dr Michael Walsh, WAHRI, University of Western Australia 10. Evolution of glyphosate resistance in annual ryegrass: Effects of cutting rates, Roberto Busi and Stephen B. Powles, Western Australian Herbicide Resistance Initiative, School of Plant Biology, The University of Western Australia 11. Metribuzin and other herbicides pre-sowing of lupins, Peter Newman, Department of Agriculture and Food 12. Crop topping lupins with glufosinate gives poor control of ryegrass seed set, Peter Newman, Department of Agriculture and Food 13. Brome grass has developed multiple resistance to Group B and C herbicides, Dr Abul Hashem, Dr Catherine Borger and Dr Shahab Pathan, Department of Agriculture and Food 14. Effect of sowing methods, Logran® and Metribuzin on weeds and wheat grain yield, Alexandra Douglas and Abul Hashem, Department of Agriculture and Food 15. Effect of alternative Group K herbicides on control of on-row annual ryegrass in wide row lupins, Dr Abul Hashem1, Ray Fulwood2 and Chris Roberts1, 1Department of Agriculture and Food, 2Farmer, Meckering, Western Australia 16. Control and seed production of annual ryegrass in wide row lupins within the Western Australian wheatbelt, Abul Hashem1,6, Alex Douglas1,6, Shahab Pathan1, Glen Riethmuller1,6 and 1,6Sally Peltzer, Department of Agriculture and Food, 6CRC Australian Weed Management 17. Effective weed control in wide row lupins, Glen Riethmuller, Abul Hashem and Shahab Pathan, Department of Agriculture and Food, and CRC Australian Weed Management 18. Slender iceplant control, Lorinda Hunt1, John Borger1, Meir Altman1,4 and Dr Ed Barrett-Lennard1,4, Department of Agriculture and Food, Western Australia1, University of Western Australia and Future Farm Industries CRC4 19. Chemical and non-chemical weed control – a European perspective, Glen Riethmuller, Department of Agriculture and Food 20. Mouldboard ploughing shows promise on sand, Peter Newman, Stephen Davies and Sally Peltzer, Department of Agriculture and Food 21. Weed seed head trimming, Glen Riethmuller and Abul Hashem, Department of Agriculture and Food 22. A survey of summer weed incidence and distribution across the WA wheatbelt, Pippa Michaela, Bill McLeodb, Catherine Borgerb and Alex Douglasb, aCurtin University of Technology, bDepartment of Agriculture and Food 23. Herbicide tolerance of field pea varieties, Harmohinder Dhammu and Mark Seymour, Department of Agriculture and Food 24. Herbicide tolerance of current/new wheat varieties, Dr Harmohinder Dhammu, Department of Agriculture and Food 25. Herbicide tolerance of new oat varieties, Harmohinder Dhammu, Vince Lambert and Chris Roberts, Department of Agriculture and Food 26. Herbicide tolerance of saltbush and bluebush, Lorinda Hunt1, John Borger1, Meir Altman1,4 and Dr Ed Barrett-Lennard1,4, Department of Agriculture and Food1, University of Western Australia and Future Farm Industries CRC4 27. A review of 2,4-D formulations and vapour drift, John H. Moore, Department of Agriculture and Food 28. Movement of 2,4-D butyl ester and the dose response of three formulations of 2,4-D on canola, John H. Moore, Department of Agriculture and Food 29. Pathways to registration – Improving pesticide research outcomes, Dr Rohan Rainbow, Manager Crop Protection, Grains Research and Development Corporatio