STUDIES ON BIOACTIVE POTENTIAL OF STREPTOMYCES SPP. (KX710212). ISOLATED FROM ENVIRONMENTAL POLLUTED SAMPLE, RANIPET, VELLORE, INDIA

ABSTRACTObjective: Natural products from bacterial origin played and still play a valuable role in drug discovery and provide diverse health-promotingcompounds that help to cure or alleviate serious diseases.Methods: A total of 4 actinobacterial strains were isolated from 2 different environmental polluted soil samples collected from Ranipet, Vellore, TamilNadu, India. Of 15 isolates screened for antibacterial property, 4 isolates (PS1, PS2, and PS3) were found potent and characterized phenotypically andgenotypically.Results: The 16S rDNA gene sequences of potent strain showed 97% similarity with Streptomyces spp. and termed as Streptomyces sp. The bioactivitywas revealed by antibacterial and antioxidant activity.Conclusion: Hence, these findings provide scientific evidence to validate the pharmacological use and have the potential contribution in the discoveryof new lead that play a key role in the field of therapeutics.Keywords: Actinomycetes, Bioactive compounds, Antibacterial property

Changes in growth and pigment content in Sweet potato by triadimefon and hexaconazole

            Triadimefon and hexaconazole are triazole group of fungicides have plant growth regulating properties. The present investigation aimed to study the growth regulating effect of these compounds on sweet potato (Ipomoea batatas L.). Each plant was treated with one litre of aqueous solutions containing 15mg L-1 triadimefon and 10mg L-1 hexaconazole on 40 and 70 days after planting (DAP) by soil drenching. The growth parameters of the plants were estimated on 45, 60, 75, 90 and 105 DAP.  Among the triazole, hexaconazole inhibited the number of leaves, total leaf area, stem length, internodal length, leaf and stem dry weight, relative shoot growth rate significantly when compared to triadimefon.  Triazole (chiefly available and plant control and yield production) treatments increase the root length, root and tuber dry weight, relative tuber growth rate, net assimilation rate root/shoot ratio, leaf dry weight per unit area, chlorophyll and carotenoid content

The binding requirements of monkey brain lysosomal enzymes their immobilised receptor protein

The lysosomal enzyme binding protein (receptor protein) isolated from monkey brain was immobilised on Sepharose 4B and used to study the binding of brain lysosomal enzymes. The immobilised protein could bind ß-D-glucosaminidase, α-D-mannosidase, α -L-fucosidase and ß-D-glucuronidase. The bound enzymes could be eluted either at an acid pH of 4.5 or by mannose 6-phosphate but not by a number of other sugars tested. Binding could be abolished by prior treatment of the lysosomal enzymes with sodium periodate. Alkaline phosphatase treatment of the enzymes did not prevent the binding of the lysosomal enzymes to the column but decreased their affinity, as seen by a shift in their elution profile, when a gradient elution with mannose 6-phosphate was employed. These results suggested that an 'uncovered' phosphate on the carbohydrate moiety of the enzymes was not essential for binding but can enhance the binding affinity

Brush Plated Copper Gallium Sulphide Films and their Properties

Copper gallium sulphide films were deposited for the first time by the brush plating technique at different electrolyte temperatures in the range of 30°C - 80°C and at a constant deposition current density of 5.0 mA cm-2. X-ray diffractograms of the films are single phase with chalcopyrite structure. EDAX measurements indicated that the Cu/Ga ratio decreased from 1.29 to 1.00 as the electrolyte temperature increased from 30°C - 80°C. The grain size increased with increase of electrolyte temperature. The grain size increases from 100 nm to 300 nm as the electrolyte temperature increases

COMPARATIVE STUDY OF ANTIMICROBIAL ACTIVITY ON FRESH AND DRIED Zingiber officinale Rosc

The present study to investigate the antimicrobial activity, from rhizome fresh and dried Zingiber officinale Rosc. In the present study to observe the antibacterial activity using the microorganisms such as E. coli, Staphylococcus aureus, K. pneumoniae and Pseudomonas aeroginosa were studied by using disc diffusion method. The maximum zone of inhibition were observed in K. pneumoniae (25 mm), followed by Staphylococcus aureus (24 mm), Pseudomonas aeroginosa and E. coli each showed 22 mm. The antifungal activity carried out by using the microorganisms Aspergillus flavus, A. terreus, Penicillum sp and Fusarium sp were studied by using agar well diffusion method. The maximum zone of inhibition were observed at the concentration of 100 µg of fresh sample against Fusarium sp (14 mm) followed by A. flavus (12 mm), A. terreus (10 mm) and Penicillum sp (10 mm). &nbsp

Design and evaluation of timolol maleate ocuserts

A remarkable attempt was made to prepare timolol maleate ocuserts, which is significant beta adreno receptor antagonist, by the aid of different of different ratio of composition of polymers such as EC, HPMC and Eudragit RS 100. Twelve batches of suitable ophthalmic films formulated by the method of solvent casting technique. Out of which the best formulation was found out the zero order release was observed in batch and was considered as the least drug releasing one. The formulated ocuserts were flexible, uniform and was meant for physic-chemical evaluator parameters, in vitro drug release profile and in vivo evaluation made on male rabbit

Controlled drug delivery of diltiazem hydrochloride as transdermal patches: a novel approach on formulation evaluation in vitro and in vivo parameters

A significant effort was done to formulate transdermal patches (Paranjothi 1998) of Diltiazem Hydrochloride (DH), a benzothiazepine calcium channel blocker, mainly meant for the treatment of hypertension, chronic stable angina pectoris; by using hydroxy ethyl cellulose, ethyl cellulose and Eudragit RLPO. Six batches of transdermal patches were prepared by solvent casting technique in which the best formulation was found out. The polymers HEC, EC and Eudragit RLPO were incorporated with Diltiazem Hydrochloride in various proportions, out of which the best formulation on the ratio [HEC: EC: EUDRAGIT RLPO-1:1:2] with the drug was determined. The prepared transdermal patches were spherical, uniform in shape and white in color. The obtained transdermal films were evaluated for physico-chemical characteristics, in vitro release profile and in vivo evaluation in albino mice. Higuchis plot studies revealed that the predominant mechanism of drug release was diffusion