Effected by dietary nucleotide on changes in intestinal Morphology, growth and fatty acid profile of whiteleg shrimp (Litopenaeus vannamei)

The effects of dietary nucleotide (0.2%) on the growth, intestinal morphology as well as fatty acid profile of the whiteleg shrimp, Litopenaeus vannamei was investigated in Bushehr province (Delvar) for a 5-week feeding trial. The experiment was carried out in triplicate in circular PVC tanks of 300L capacity. Each tank was randomly filled with 25 shrimps weighting on average 3.21±0.03g. Added dietary nucleotide significantly improved growth factors such as 10% weight increase, 7.97% increase in specific growth rate (SGR), 11.32% increase in protein efficiency ratio (PER), 9.64% decrease in feed conversion ratio (FCR) and 14:1n5, 20:3n3 and DHA fatty acids. However, no significant differences were evident in survival, carapace length gain, daily feed consumption and saturate fatty acid (SFA), monounsaturated fatty acid (MUFA), poly unsaturated fatty acid (PUFA) content, n-3/n-6 as well as EPA+DHA fatty acids between the two treatments. Intestine epithelium cells height (IECH) in the abdominal first section were significantly higher in shrimp fed by dietary nucleotide

Histology of intestine and fatty acid profile of Pacific white shrimp (Litopenaeus vannamei) effected by dietary inulin

The effects of inulin were studied on the growth indices, intestinal morphology as well as fatty acid profile of the Pacific white shrimp, Litopenaeus vannamei with average weight 3.21±0.03 g for 5 weeks. The experiment was carried in triplicate, was conducted in circular PVC tanks of 300 L capacity with 25 shrimp per tank at the Aquaculture Center (Delvar, Bushehr province). Inulin was added to the diets at a 2 levels (0 and 2 percent). The shrimp were fed the experimental diets to visual satiety five times a day at 08:00, 12:00, 16:00, 20:00 and 24:00 h. No significant differences in growth performances (such as survival, feed conversion ratio (FCR), specific growth rate (SGR), Protein efficiency ratio (PER)) was observed, however, Dietary inulin brought about significantly improved 20:3n3 fatty acid content between the two treatments (P>0.05). Intestine epithelium cells height in the abdominal sections significantly (P< 0.05) were higher in shrimp fed by dietary inulin. The results of this study showed that supplement of 2% of inulin has positive effects on fatty acid profile and intestinal morphology in Litopenaeus vannamei

Effect of different levels of dietary nucleotide on growth and haemolymph biochemical parameters of female Litopenaeus vannamei (Boone, 1931) during maturation and Eyestalk Ablation

In this study, the effect of different dietary nucleotide levels (0, 0.2, 0.4 and 0.6%) was investigated on growth indices and some haemolymph biochemical parameters of Litopenaeus vannamei female broodstock during 30 days. Broodstocks were fed twice with fresh food and twice with concentrate feed). After 3 weeks of feeding, eyestalk of broodstocks was ablated with hot scissors. Hemolymph was sampled for glucose, total cholesterol, triglyceride, calcium, albumin, total soluble proteins, and high density lipoprotein on the first day of the trial, 21 days (before eyestalk ablation) and 30 days (9 days after eyestalk ablation) after starting the feeding trial. Water parameters including temperature, dissolve oxygen, salinity and pH recorded daily as 27.79±1.24˚C, 5.78±0.41 ppm, 31.82±3.1 ppt and 7.9±0.18, respectively. Weight gain, specific growth rate and daily growth rate in samples treated by dietary nucleotide showed no significant difference (p>0.05) compared to control group. Significant increase (p<0.05) in glucose, total cholesterol, triglyceride, albumin and total protein were observed in all groups compared to control group. In conclusion 0.4 % nucleotide can improve the dietary sexual maturation of Litopenaeus vannamei

Genetic analysis of sand whiting Sillago sihama from north Persian Gulf based on cytochrome oxidase C subunit I sequences

Silaginidae family fish from Perciforms, are appropriate candidates for shallow water and coastal aquaculture. At least three species of this family represents in the Persian Gulf. Genetic analysis of Sand whiting Sillago sihama , the most common species of family performed using COI gene. During the present study 10 samples were collected from Bushehr and Hormozgan provinces coastal waters. DNA extracted by modified CTAB method. Polymerase chain reactions were performed using universal primers - FISHF1 and FISHR1-. Sequencing results showed a 627 bp amplified fragment. Performing BLAST supported high identity to Sillago sihama species; hence morphometric identification confirms molecular barcoding. Genetic distance of 0.02 was calculated between samples of two areas based on Kimura 2- parameter using Mega software. Constructed phylogenetic tree using neighbor joining method whereas the Acanthopagrus latus sequences was used as an outgroup revealed no differentiation between two stations samples. In conclusion based on the results of the present study, the gene flow was high among studied samples and no significant differentiation was observed between Bushehr and Hormozgan samples. In conclusion no discrete populations differentiated based on the results of the present study

Dietary nucleotide mixture effects on reproductive and performance, ovary fatty acid profile and biochemical parameters of female Pacific shrimp Litopenaeus vannamei

The effects of different dietary nucleotide mixture (NT) levels (0, 2, 4 and 6 g/kg) were investigated on the reproductive performance, fatty acid profile and biochemical parameters in Litopenaeus vannamei female that were co‐fed with fresh food (two times daily) and experimental diets (two times daily) for 30 days. Sampling was carried out at the first day of the trial, before eyestalk ablation (ESA) (day 21) and after ESA (day 30). Reproductive performance parameters including the hepatopancreatic index, absolute fecundity, egg diameter and latency period were higher in the NT‐supplemented groups than the control group (p < .05). Total n‐3 polyunsaturated fatty acids in the ovaries, especially eicosapentaenoic acid, were higher in the NT‐supplemented groups than in the control (222.6 ± 7.3 versus 204.7 ± 3.0 mg/g extracted lipid) (p < .05). Total hemocyte count, granular cells, plasma glucose (Glu), total protein (TP), calcium (Ca2+), total cholesterol (Chol), triglyceride (Tg) and high‐density lipoprotein (HDL) levels were significantly increased in the NT‐supplemented groups in comparison with the control group (p < .05). After ESA, the concentration of plasma Glu, TP, Chol and Tg significantly decreased, but HDL levels increased in all groups (p < .05). These results suggested that the application of dietary NT may present a novel strategy for increasing reproductive performance and health status in L. vannamei.info:eu-repo/semantics/acceptedVersio