The effect of N-stearoylethanolamine on the lipid composition of the rat testes and testosterone level during the early stages of streptozotocin-іnduced diabetes

Diabetes is a metabolic disorder with multiorgan complications, including reproductive system dysfunction where lipid imbalance of germ cells play an important role. N-stearoylethanolamine (NSE) shows a modulatory effect on the lipid composition under different pathologies. Therefore, the aim of our study was to investigate the NSE effect on the testes lipid composition and testosterone level in plasma of diabetic rats. Diabetes was induced in Sprague-Dawley rats by a single streptozotocin injection (50 mg/kg). Animals with glucose levels of 8-12 mmol/l were further selected. NSE was administrated to rats (50 mg/kg) for 10 days at 1.5 months after the streptozotocin injection. The rat testes were used for lipid analysis, namely, phospholipid level, fatty acid methyl esters and plasma testosterone estimation. NSE administration to diabetic rats triggered normalization of total and individual phospholipid content, as well as composition of free and phospholipids fatty acids in the rat testes. In addition, the testosterone content showed a slight increase under the action of NSE. Our results showed that the early stages of diabetes caused destructive changes in rat testes that may induce a decrease in future testicular function. NSE administration to diabetic rats normalized the lipid content of rat testes and was correlated with an increased testosterone level. NSE induced the restoration of testes structure and function during the early stages of streptozotocin-іnduced diabetes in rats

Obstetric and perinatal sequelae of labor in women with breech presentation

The aim of the study is to characterise the somatic and obstetric and gynaecological anamnesis, as well as to assess the features of pregnancy, childbirth and newborns’ condition in women with breech presentation. Materials and methods. The medical documents of 2980 patients with breech presentation in the period from 2022 to 2024 by materials of the Municipal Non-Commercial Enterprise “Zaporizhzhia Regional Clinical Children’s Hospital” of the Zaporizhzhia Regional Council were analysed. The study group included 65 histories of pregnancy and childbirth (primary accounting documentation form No. 096/o) and 68 newborn medical records (primary accounting documentation form No. 097/o). The used research methods were data from: obstetric, gynaecological, and somatic history; management of pregnancy, childbirth, initial assessment of newborns; results of newborn observation during their stay in the department of the hospital. Results. When analysing medical records, breech presentation was diagnosed in 65 pregnant women (2.2 %). In 60 women with breech presentation, the delivery ended in a caesarean section (92.3 %), and in 5 (7.7 %) women with breech presentation, the delivery went through the natural birth canal. In the case of breech presentation, which accounted for 73.3 %, all pregnant women were delivered by caesarean section (88.6 % as planned, 11.4 % as urgent due to fetal distress). Other indications for caesarean section included: breech presentation of the first fetus in multiple pregnancy, uterine scar after caesarean section, pelvic ring deformity, HIV infection with a high viral load and premature detachment of a normally located placenta. Conclusions. When analysing the anamnesis of women with breech presentation, the vast majority of extragenital pathology (76.9 %) was cardiovascular and endocrine diseases. An analysis of the course of gestation in women with breech presentation revealed that 40 % had such pregnancy complications as hypertensive disorders, pre- and post-eclampsia, anaemia, fetal distress, and premature rupture of membranes. Complications of the perinatal period accounted for 30.9 % (neonatal jaundice, congenital infections and parasitic diseases, neonatal encephalopathy, prematurity, etc.)

Preventive effect of N-stearoylethanolamine on memory disorders, blood and brain biochemical parameters in rats with experimental scopolamine-induced cognitive impairment

The impairment of cognitive functions is the most studied medical and social problem nowadays. The aim of this study was to evaluate the protective effects of N-stearoylethanolamine (NSE) on memory state, blood and brain biochemical parameters in rats under scopolamine-induced cognitive impairment. The results of this study shown that NSE administration to rats per os (5 mg/kg, 5 days, during last 3 days NSE was administrated 20 min prior to scopolamine injection (1 mg/kg, once daily for 3 days, intraperitoneally)) prevented the development of memory impairment. In particular, NSE action was associated with the prevention of increase in acetylcholinesterase activity, changes in phospholipid, free and esterified cholesterol level in hippocampus and frontal cortex, and disruption in pro-/antioxidant balance in blood and studied brain sections. Considering the above mentioned biological effects, NSE is a promising drug candidate for integrative therapy of cognitive impairment of different profiles

ETHNIC DIFFERENCES IN THE LENGTH OF CAG REPEATS IN THE ANDROGEN RECEPTOR GENE AND TESTOSTERONE LEVELS IN YOUNG MEN OF THE SIBERIAN REGION OF RUSSIA

The aim of the study was to identify ethnic differences in the number of CAG repeats of the&#x0D; androgen receptor gene (AR), anthropometric and hormonal indicators in men of three ethnic groups&#x0D; (Slavs, Buryats and Yakuts) living in the Russa. The androgen receptor mediates the effects of&#x0D; androgens on a wide range of organs and tissues in men. The AR is characterized by polymorphism&#x0D; of CAG repeats, which can be an ethno-dependent trait and determine functional and&#x0D; morphogenetic effects of testosterone. The study established the ethnic differences in the length of&#x0D; CAG repeats between the Slavs, Buryats and Yakuts (23, 24, 25 triplets, respectively), as well as in&#x0D; anthropometric and hormonal indicators. LH concentrations were higher in Buryats than in Slavs or&#x0D; Yakuts, but the testosterone level was the lowest in Buryats compared to Slavs or Yakuts.</jats:p