15 research outputs found
Number of root tips and number of secondary roots of red clover in hydroponic system.
<p>Roots were assessed at the harvest (n = 6, mean ± standard deviation). Exposure concentrations (1<2) are described in detail in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0155111#pone.0155111.t002" target="_blank">Table 2</a>.</p
Relative growth rates of <i>R</i>. <i>trifolii</i> in YMB medium over a 32 h exposure assessed by optical density.
<p><i>R</i>. <i>trifolii</i> growth rates were assessed in medium containing different actual concentrations and qualities of TiO<sub>2</sub> NPs (P25 filled diamond, E171 filled triangle, NNM TiO<sub>2</sub> inverted triangle, ZnSO<sub>4</sub>*7H<sub>2</sub>O circle) during the 34 h (n = 4). Stars indicate significant (p<0.05) differences from the control. Exponential growth curves are shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0155111#pone.0155111.s001" target="_blank">S1 Appendix</a>.</p
Analytical data of the TiO<sub>2</sub> NPs suspended in YMB medium.
<p>Suspensions for the <i>R</i>. <i>trifolii</i> exposure experiment were assessed at the start of the experiment (t = 0) and at the end, i.e., after 34 h (n = 3).</p
Effects of Titanium Dioxide Nanoparticles on Red Clover and Its Rhizobial Symbiont
<div><p>Titanium dioxide nanoparticles (TiO<sub>2</sub> NPs) are in consideration to be used in plant protection products. Before these products can be placed on the market, ecotoxicological tests have to be performed. In this study, the nitrogen fixing bacterium <i>Rhizobium trifolii</i> and red clover were exposed to two TiO<sub>2</sub> NPs, i.e., P25, E171 and a non-nanomaterial TiO<sub>2</sub>. Growth of both organisms individually and their symbiotic root nodulation were investigated in liquid and hydroponic systems. While 23 and 18 mg l<sup>-1</sup> of E171 and non-nanomaterial TiO<sub>2</sub> decreased the growth rate of <i>R</i>. <i>trifolii</i> by 43 and 23% respectively, P25 did not cause effects. Shoot length of red clover decreased between 41 and 62% for all tested TiO<sub>2</sub> NPs. In 21% of the TiO<sub>2</sub> NP treated plants, no nodules were found. At high concentrations certain TiO<sub>2</sub> NPs impaired <i>R</i>. <i>trifolii</i> as well as red clover growth and their symbiosis in the hydroponic systems.</p></div
Length of the main root (white) and shoot (grey) at harvest (t = 4 weeks).
<p>Lengths are shown for the control, the different TiO<sub>2</sub> NP treatments in two concentrations 1 (low) and 2 (high) that are described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0155111#pone.0155111.t002" target="_blank">Table 2</a>, and the 16.1 mg l<sup>-1</sup> ZnSO<sub>4</sub> treatment (a) in presence of <i>R</i>. <i>trifolii</i> or (b) without <i>R</i>. <i>trifolii</i>. Significant (p<0.05) differences to the respective control (n = 6) are indicated with asterisks above the standard deviation error bars for shoots, and below the error bars for roots. The results of the same treatments with and without <i>R</i>. <i>trifolii</i> are not significantly different and neither were the controls, but the root length of ZnSO<sub>4</sub> with and without <i>R</i>. <i>trifolii</i> was different (p = 0.005).</p
Scanning electron microscopy image of dried red clover root.
<p>Root surface from a 24 mg l<sup>-1</sup> E171 treated plant is shown. The insert shows a magnification of 1, and from the spots 2 and 3 (+) X-ray fluorescence spectra were prepared revealing that spot 2 did not contain titanium while spot 3 contained titanium.</p
<sup>15</sup>N content (% of total N) of red clover shoots.
<p>Results are shown for the control, the different TiO<sub>2</sub> NPs in two concentrations (1 = low, 2 = high) as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0155111#pone.0155111.t001" target="_blank">Table 1</a> and the ZnSO<sub>4</sub> treatment (a) with addition of <i>R</i>. <i>trifolii</i> and (b) without <i>R</i>. <i>trifolii</i> inoculation. Error bars indicate standard deviations, asterisks show significant differences compared to the control (p<0.05) and number of replications are indicated on the graph (n). Number of replications varied because not for all samples the required amount of shoot biomass for <sup>15</sup>N measurement was available.</p
Total TiO<sub>2</sub> suspended or sedimented in the hydroponic system.
<p>Red clover was exposed (n = 3) over 162 h to the two nanoparticles P25 and E171. TiO<sub>2</sub> amounts of the pooled stock suspension is shown at t = 0 in black. TiO<sub>2</sub> amounts of the top (white, 17 ml, in contact with roots) and bottom part (grey, 3 ml, including precipitate) are shown. Differences of the total TiO<sub>2</sub> NP amount (bottom and top part together) to the total Ti amount at t = 0 are indicated with asterisks (p<0.05). Error bars indicate standard deviations (n = 3).</p
Analytical data of the TiO<sub>2</sub> NP suspensions in FĂĄhraeus medium (FM).
<p>Suspensions of the hydroponic experiment were explored over four weeks (n = 3).</p