Growth Response of Soybean Varieties to

This study aims to determine the growth response of the vegetative stadia of three soybean varieties which are widely used in East Java on acid soils that have been given biological fertilizers from various Trichoderma isolates. The experiment was arranged factorially using a completely randomized design. The first factor was the variety consisting of Detam 3, Detam 4, Gema, Dering 1, and Burangrang. The second factor was Trichoderma isolates formulated as bio fertilizers, consisting of Tc-Jjr-02, Tc-Pjn-01, and Tc-Jro-01. Overall this experiment has 60 experimental units. Data were analyzed by variance analysis (ANOVA) followed by an honest significant difference test at the level of 5%. The results showed that there was no interaction between soybean varieties and Trichoderma isolates formulated in bio fertilizer. Soybean varieties significantly affected plant height, wet weight, and dry weight of roots and stover at 21 days after planting. The Burangrang variety shows the best growing ability in acid soils. There was no interaction between soybean varieties and Trichoderma bio fertilizers. Trichoderma isolate Tc-Jjr-02 increased the wet weight and dry weight of the roots and roots by 48.2 and 54.5%, respectively, and 38.9 and 48.2% compared to without Trichoderma. Trichoderma application maintains soil acidity between pH 4.50-4.67

Biomass Extract of

This study aims to determine the type of metabolites contained in the extraction of Ipomea carnea canopy biomass and its inhibitory effect in vitro against Trichoderma asperellum Tc-Jjr-02 which has been tested as an active ingredient in bio fungicides and biofertilizers. The initial stage of the research is the determination of the metabolites contained in the extract with methanol solvent through Gas Chromatography-Mass Spectrophotometry (GCMS) analysis. The next step was an in vitro test of the extract's inhibitory power to the growth of T. asperellum fungal colonies during the incubation period of up to 96 hours. The results of GCMS analysis of extracts with methanol solvents obtained 24 types of metabolites, some of which can inhibit fungal growth. Crude extract of canopy biomass of I. carnea at a concentration of 2% inhibited the growth of all Trichoderma isolates by 20.97±3.36% (Tc-Jjr-02), 38.15±32.59% (Tc-Sba-01), and 45.30±1.86% (Tc-Jro-01), while at a concentration of 20% inhibited isolates Tc-Sba-01 and Tc-Jro-01 were 35.19±4.90% and 42.59±1.85% in vitro at 96 hours after inoculation, respectively. The isolate of T. esperellum Tc-Jjr-02 has the potential to be formulated with I. carnea biomass as compost in the future

Effect of trichoderma formulated with cultivated oyster mushroom waste toward the growth and yield of shallot (Allium ascalonicum L.)

The utilization of oyster mushroom waste as an organic fertilizer for onion cultivation in former rice fields frequently encounters soil acidity problems. This experiment aims to determine the effect of administering Trichoderma biofertilizer, formulated with Oyster Mushroom Waste (OMW) on the growth and yield of shallots. The experiment was carried out in a greenhouse setting and a completely randomized design with four replications was employed, each experimental unit having six plants. Seeds were planted in a 5 kg capacity polybag containing sterile planting media soil of pH 4.85. The experimental treatments consisted of three types of Trichoderma biofertilizers, each containing a different isolate of the fungi (Tc-Jjr-02, Tc-Pjn-01, and Tc-Clk-02), oyster mushrooms waste fertilizer and the only soil. Each isolates formulated in oyster mushrooms waste through the biofertilizer treatment contained a uniform spore population of 0.375x106 CFU.g-1. Data were collected on plant height, number of leaves per plant, fresh and dry mass of stover, number of tillers per plant, tuber weight, tuber diameter, soil pH, and the population of each Trichoderma isolate at harvest time. The data were analyzed using a one-way analysis of variance (ANOVA). The significant differences between treatments were determined using the Honest Significant Difference test at the 5% level. Based on the growth and yield, the amount of increase for each treatment was calculated compared to the treatment of oyster mushrooms waste fertilizer. Subsequently, the types of isolate and their phylogenetic classes were determined. The results showed that the Trichoderma biofertilizer formulated in OMW affected plant height and number of leaves at 7-28 DAP (p<0.05), fresh weight and dry weight of stover, number of tuber shoot, and tuber weight (p<0.01), tuber diameter (p<0.05), soil pH at six weeks after planting and one week before harvest (p<0.05), and also Trichoderma population (p<0.05). These three types of this biofertilizer can increase stover fresh weight of shallot between 13.97-52.05%, dry stover weight 67.76-151.42%, number of tillers 44.75-47.00%, heavy fresh tuber 20.31-28.13%, and tuber diameter 3.13-10.97%. The three isolates of the biofertilizer agent were identical to Trichoderma asperellum, where the Tc-Jjr-02 isolate showed the best performance in assisting the growth and production of shallot plants in acidic soils