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Single fault detection in combinational logic circuits
The material in this paper is divided into the following four chapters for convenience. Chapter 1 explains, how the idea of fault testing changed from testing the machine instructions to testing the hardware in logic circuits. Chapters 2, 3 and 4 present the different approaches considered, namely:
1. Path sensitizing and D-Algorithm method.
2. Tabular method.
3. Boolean difference method
Plasmon resonances of highly doped two-dimensional MoS2
The exhibition of plasmon resonances in two-dimensional (2D) semiconductor compounds is desirable for many applications. Here, by electrochemically intercalating lithium into 2D molybdenum disulfide (MoS2) nanoflakes, plasmon resonances in the visible and near UV wavelength ranges are achieved. These plasmon resonances are controlled by the high doping level of the nanoflakes after the intercalation, producing two distinct resonance peak areas based on the crystal arrangements. The system is also benchmarked for biosensing using bovine serum albumin. This work provides a foundation for developing future 2D MoS2 based biological and optical units
Designing optoelectronic properties by on-surface synthesis: formation and electronic structure of an iron-terpyridine macromolecular complex
Supramolecular chemistry protocols applied on surfaces offer compelling
avenues for atomic scale control over organic-inorganic interface structures.
In this approach, adsorbate-surface interactions and two-dimensional
confinement can lead to morphologies and properties that differ dramatically
from those achieved via conventional synthetic approaches. Here, we describe
the bottom-up, on-surface synthesis of one-dimensional coordination
nanostructures based on an iron (Fe)-terpyridine (tpy) interaction borrowed
from functional metal-organic complexes used in photovoltaic and catalytic
applications. Thermally activated diffusion of sequentially deposited ligands
and metal atoms, and intra-ligand conformational changes, lead to Fe-tpy
coordination and formation of these nanochains. Low-temperature Scanning
Tunneling Microscopy and Density Functional Theory were used to elucidate the
atomic-scale morphology of the system, providing evidence of a linear tri-Fe
linkage between facing, coplanar tpy groups. Scanning Tunneling Spectroscopy
reveals highest occupied orbitals with dominant contributions from states
located at the Fe node, and ligand states that mostly contribute to the lowest
unoccupied orbitals. This electronic structure yields potential for hosting
photo-induced metal-to-ligand charge transfer in the visible/near-infrared. The
formation of this unusual tpy/tri-Fe/tpy coordination motif has not been
observed for wet chemistry synthesis methods, and is mediated by the bottom-up
on-surface approach used here
A widespread class of reverse transcriptase-related cellular genes
Author Posting. © The Author(s), 2011. This is the author's version of the work. It is posted here by permission of National Academy of Sciences for personal use, not for redistribution. The definitive version was published in Proceedings of the National Academy of Sciences of the United States of America 108 (2011):20311-20316, doi:10.1073/pnas.1100266108.Reverse transcriptases (RTs) polymerize DNA on RNA templates. They fall into several structurally
related but distinct classes, and form an assemblage of RT-like enzymes which, in addition to RTs, also
includes certain viral RNA-dependent RNA polymerases (RdRP) polymerizing RNA on RNA templates. It
is generally believed that most RT-like enzymes originate from retrotransposons or viruses and have no
specific function in the host cell, with telomerases being the only notable exception. Here we report on the
discovery and properties of a novel class of RT-related cellular genes collectively named rvt. We present
evidence that rvt are not components of retrotransposons or viruses, but single-copy genes with a
characteristic domain structure, may contain introns in evolutionarily conserved positions, occur in
syntenic regions, and evolve under purifying selection. These genes can be found in all major taxonomic
groups including protists, fungi, animals, plants, and even bacteria, although they exhibit patchy
phylogenetic distribution in each kingdom. We also show that the RVT protein purified from one of its
natural hosts, Neurospora crassa, exists in a multimeric form and has the ability to polymerize NTPs as
well as dNTPs in vitro, with a strong preference for NTPs, using Mn2+ as a cofactor. The existence of a
previously unknown class of single-copy RT-related genes calls for re-evaluation of the current views on
evolution and functional roles of RNA-dependent polymerases in living cells.This work was supported by NSF grant MCB-0821956 to I.A
The Type III Secreted Protein BspR Regulates the Virulence Genes in Bordetella bronchiseptica
Bordetella bronchiseptica is closely related with B. pertussis and B. parapertussis, the causative agents of whooping cough. These pathogenic species share a number of virulence genes, including the gene locus for the type III secretion system (T3SS) that delivers effector proteins. To identify unknown type III effectors in Bordetella, secreted proteins in the bacterial culture supernatants of wild-type B. bronchiseptica and an isogenic T3SS-deficient mutant were compared with iTRAQ-based, quantitative proteomic analysis method. BB1639, annotated as a hypothetical protein, was identified as a novel type III secreted protein and was designated BspR (Bordetella
secreted protein regulator). The virulence of a BspR mutant (ΔbspR) in B. bronchiseptica was significantly attenuated in a mouse infection model. BspR was also highly conserved in B. pertussis and B. parapertussis, suggesting that BspR is an essential virulence factor in these three Bordetella species. Interestingly, the BspR-deficient strain showed hyper-secretion of T3SS-related proteins. Furthermore, T3SS-dependent host cell cytotoxicity and hemolytic activity were also enhanced in the absence of BspR. By contrast, the expression of filamentous hemagglutinin, pertactin, and adenylate cyclase toxin was completely abolished in the BspR-deficient strain. Finally, we demonstrated that BspR is involved in the iron-responsive regulation of T3SS. Thus, Bordetella virulence factors are coordinately but inversely controlled by BspR, which functions as a regulator in response to iron starvation
Target Site Recognition by a Diversity-Generating Retroelement
Diversity-generating retroelements (DGRs) are in vivo sequence diversification machines that are widely distributed in bacterial, phage, and plasmid genomes. They function to introduce vast amounts of targeted diversity into protein-encoding DNA sequences via mutagenic homing. Adenine residues are converted to random nucleotides in a retrotransposition process from a donor template repeat (TR) to a recipient variable repeat (VR). Using the Bordetella bacteriophage BPP-1 element as a prototype, we have characterized requirements for DGR target site function. Although sequences upstream of VR are dispensable, a 24 bp sequence immediately downstream of VR, which contains short inverted repeats, is required for efficient retrohoming. The inverted repeats form a hairpin or cruciform structure and mutational analysis demonstrated that, while the structure of the stem is important, its sequence can vary. In contrast, the loop has a sequence-dependent function. Structure-specific nuclease digestion confirmed the existence of a DNA hairpin/cruciform, and marker coconversion assays demonstrated that it influences the efficiency, but not the site of cDNA integration. Comparisons with other phage DGRs suggested that similar structures are a conserved feature of target sequences. Using a kanamycin resistance determinant as a reporter, we found that transplantation of the IMH and hairpin/cruciform-forming region was sufficient to target the DGR diversification machinery to a heterologous gene. In addition to furthering our understanding of DGR retrohoming, our results suggest that DGRs may provide unique tools for directed protein evolution via in vivo DNA diversification
Differential Expression of Type III Effector BteA Protein Due to IS481 Insertion in Bordetella pertussis
BACKGROUND: Bordetella pertussis is the primary etiologic agent of the disease pertussis. Universal immunization programs have contributed to a significant reduction in morbidity and mortality of pertussis; however, incidence of the disease, especially in adolescents and adults, has increased in several countries despite high vaccination coverage. During the last three decades, strains of Bordetella pertussis in circulation have shifted from the vaccine-type to the nonvaccine-type in many countries. A comparative proteomic analysis of the strains was performed to identify protein(s) involved in the type shift. METHODOLOGY/PRINCIPAL FINDING: Proteomic analysis identified one differentially expressed protein in the B. pertussis strains: the type III cytotoxic effector protein BteA, which is responsible for host cell death in Bordetella bronchiseptica infections. Immunoblot analysis confirmed the prominent expression of BteA protein in the nonvaccine-type strains but not in the vaccine-type strains. Sequence analysis of the vaccine-type strains revealed an IS481 insertion in the 5' untranslated region of bteA, -136 bp upstream of the bteA start codon. A high level of bteA transcripts from the IS481 promoter was detected in the vaccine-type strains, indicating that the transcript might be an untranslatable form. Furthermore, BteA mutant studies demonstrated that BteA expression in the vaccine-type strains is down-regulated by the IS481 insertion. CONCLUSION/SIGNIFICANCE: The cytotoxic effector BteA protein is expressed at higher levels in B. pertussis nonvaccine-type strains than in vaccine-type strains. This type-dependent expression is due to an insertion of IS481 in B. pertussis clinical strains, suggesting that augmented expression of BteA protein might play a key role in the type shift of B. pertussis
A Molecular and Co-Evolutionary Context for Grazer Induced Toxin Production in Alexandrium tamarense
Marine dinoflagellates of the genus Alexandrium are the proximal source of neurotoxins associated with Paralytic Shellfish Poisoning. The production of these toxins, the toxin biosynthesis and, thus, the cellular toxicity can be influenced by abiotic and biotic factors. There is, however, a lack of substantial evidence concerning the toxins' ecological function such as grazing defense. Waterborne cues from copepods have been previously found to induce a species-specific increase in toxin content in Alexandrium minutum. However, it remains speculative in which context these species-specific responses evolved and if it occurs in other Alexandrium species as well. In this study we exposed Alexandrium tamarense to three copepod species (Calanus helgolandicus, Acartia clausii, and Oithona similis) and their corresponding cues. We show that the species-specific response towards copepod-cues is not restricted to one Alexandrium species and that co-evolutionary processes might be involved in these responses, thus giving additional evidence for the defensive role of phycotoxins. Through a functional genomic approach we gained insights into the underlying molecular processes which could trigger the different outcomes of these species-specific responses and consequently lead to increased toxin content in Alexandrium tamarense. We propose that the regulation of serine/threonine kinase signaling pathways has a major influence in directing the external stimuli i.e. copepod-cues, into different intracellular cascades and networks in A. tamarense. Our results show that A. tamarense can sense potential predating copepods and respond to the received information by increasing its toxin production. Furthermore, we demonstrate how a functional genomic approach can be used to investigate species interactions within the plankton community
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