Latrunculin A delays anaphase onset in fission yeast by disrupting an ase1-independent pathway controlling mitotic spindle stability

It has been proposed previously that latrunculin A, an inhibitor of actin polymerization, delays the onset of anaphase by causing spindle misorientation in fission yeast. However, we show that {Delta}mto1 cells, which are defective in nucleation of cytoplasmic microtubules, have profoundly misoriented spindles but are not delayed in the timing of sister chromatid separation, providing compelling evidence that fission yeast does not possess a spindle orientation checkpoint. Instead, we show that latrunculin A delays anaphase onset by disrupting interpolar microtubule stability. This effect is abolished in a latrunculin A-insensitive actin mutant and exacerbated in cells lacking Ase1, which cross-links antiparallel interpolar microtubules at the spindle midzone both before and after anaphase. These data indicate that both Ase1 and an intact actin cytoskeleton are required for preanaphase spindle stability. Finally, we show that loss of Ase1 activates a checkpoint that requires only the Mad3, Bub1, and Mph1, but not Mad1, Mad2, or Bub3 checkpoint proteins

Weight stigma and physical health: an unconsidered ‘obesity’ cost

This is the author accepted mansuscript. The final version is available from Springer via the DOI in this recordLetter to the Editor: Response to Singh et al. (2018). Is there more to the equation? Weight bias and the costs of obesity. Canadian Journal of Public Health. https://doi.org/10.17269/s41997-018-0146-2. Online first: 26 October 2018Economic and Social Research Council (ESRC

(SNP090) Cleadus Meadows interviewed by Dorothy Noble Smith, transcribed by Sharon G. Marston

Records an interview with Cleadus Meadows, who grew up near Thoroughfare Mountain, in Madison County, Virginia. Describes daily life in the mountains, including raising livestock, the means of growing and preserving food and other aspects of the local economy, such as chestnut harvesting, tan bark and moonshine.https://commons.lib.jmu.edu/snp/1075/thumbnail.jp

Real-world shock: transition shock and its effect on new interpreters of American Sign Language and English

Real-world shock has historically been defined as a form of transition shock experienced during the transition from the academic to the professional world. It is marked by distinct phases and causes both emotional and physical stress. Previous research has found that real-world shock is experienced by new members of a variety of fields, especially training-intensive service professions such as education and medicine. However, even though the profession of American Sign Language/English interpreting is also a training-intensive service profession, there is no research that indicates whether or not new members of the profession experience similar shock to their counterparts in other professions. As such, this thesis shall attempt to determine if real-world shock is experienced by new interpreters of American Sign Language. To do so, a survey was electronically distributed to American Sign Language/English interpreters throughout the United States of America. The resulting data collected was analyzed to determine if new interpreters experience real-world shock and, if so, what the root cause of it is. It was found that interpreters of American Sign Language and English do experience real-world shock upon entering the profession. Furthermore, the shock is a result of the profession rather than from intercultural interactions with the d/Deaf community