Structural Changes in U.S. Agricultural Production and Productivity

Livestock Production/Industries,

Can the palatability of healthy, satiety-promoting foods increase with repeated exposure during weight loss?

Repeated exposure to sugary, fatty, and salty foods often enhances their appeal. However, it is unknown if exposure influences learned palatability of foods typically promoted as part of a healthy diet. We tested whether the palatability of pulse containing foods provided during a weight loss intervention which were particularly high in fiber and low in energy density would increase with repeated exposure. At weeks 0, 3, and 6, participants (n = 42; body mass index (BMI) 31.2 ± 4.3 kg/m²) were given a test battery of 28 foods, approximately half which had been provided as part of the intervention, while the remaining half were not foods provided as part of the intervention. In addition, about half of each of the foods (provided as part or not provided as part of the intervention) contained pulses. Participants rated the taste, appearance, odor, and texture pleasantness of each food, and an overall flavor pleasantness score was calculated as the mean of these four scores. Linear mixed model analyses showed an exposure type by week interaction effect for taste, texture and overall flavor pleasantness indicating statistically significant increases in ratings of provided foods in taste and texture from weeks 0 to 3 and 0 to 6, and overall flavor from weeks 0 to 6. Repeated exposure to these foods, whether they contained pulses or not, resulted in a ~4% increase in pleasantness ratings. The long-term clinical relevance of this small increase requires further study.T32 AT000815 - NCCIH NIH HH

Postnatal liver growth and regeneration are independent of c-myc in a mouse model of conditional hepatic c-myc deletion

<p>Abstract</p> <p>Background</p> <p>The transcription factor <it>c-myc </it>regulates genes involved in hepatocyte growth, proliferation, metabolism, and differentiation. It has also been assigned roles in liver development and regeneration. In previous studies, we made the unexpected observation that c-Myc protein levels were similar in proliferating fetal liver and quiescent adult liver with c-Myc displaying nucleolar localization in the latter. In order to investigate the functional role of c-Myc in adult liver, we have developed a hepatocyte-specific <it>c-myc </it>knockout mouse, <it>c-myc^fl/fl</it>;<it>Alb</it>-<it>Cre</it>.</p> <p>Results</p> <p>Liver weight to body weight ratios were similar in control and <it>c-myc </it>deficient mice. Liver architecture was unaffected. Conditional <it>c-myc </it>deletion did not result in compensatory induction of other <it>myc </it>family members or in c-Myc's binding partner Max. Floxed <it>c-myc </it>did have a negative effect on <it>Alb</it>-Cre expression at 4 weeks of age. To explore this relationship further, we used the Rosa26 reporter line to assay Cre activity in the <it>c-myc </it>floxed mice. No significant difference in Alb-Cre activity was found between control and <it>c-myc^fl/fl</it>mice. c<it>-myc </it>deficient mice were studied in a nonproliferative model of liver growth, fasting for 48 hr followed by a 24 hr refeeding period. Fasting resulted in a decrease in liver mass and liver protein, both of which recovered upon 24 h of refeeding in the c<it>-myc^fl/fl;Alb</it>-Cre animals. There was also no effect of reducing <it>c-myc </it>on recovery of liver mass following 2/3 partial hepatectomy.</p> <p>Conclusions</p> <p>c-Myc appears to be dispensable for normal liver growth during the postnatal period, restoration of liver mass following partial hepatectomy and recovery from fasting.</p

Structural Changes in U.S. Agricultural Production and Productivity

The structure of U.S. agricultural production changed dramatically during the 20th Century. Major technological innovations transformed the relationship between agricultural inputs and outputs, and contributed to rapid increases in agricultural productivity. However, evidence is mounting that suggests we have entered a new era, with substantially lower rates of productivity growth. In this article, we examine trends and spatial patterns in agricultural input use, production of outputs, and productivity. We focus on productivity growth over the period 1949–2002, and find a statistically significant slowdown in productivity growth after 1990

The Solar Neighborhood XIII: Parallax Results from the CTIOPI 0.9-m Program -- Stars with mu >= 1"/year (MOTION Sample)

We present the first set of definitive trigonometric parallaxes and proper motions from the Cerro Tololo Inter-American Observatory Parallax Investigation (CTIOPI). Full astrometric reductions for the program are discussed, including methods of reference stars selection, differential color refraction corrections, and conversion of relative to absolute parallax. Using data acquired at the 0.9-m at CTIO, full astrometric solutions and $VRIJHK_s$ photometry are presented for 36 red and white dwarf stellar systems with proper motions faster than 1\farcs0/yr. Of these, thirty three systems have the first ever trigonometric parallaxes, which comprise 41% of MOTION systems (those with proper motions greater than 1\farcs0/yr) south of $\delta$ $=$ 0 that have no parallaxes. Four of the systems are new members of the RECONS 10 pc sample for which the first accurate trigonometric parallaxes are published here: DENIS J1048-3956 (4.04 $\pm$ 0.03 pc), GJ 1128 (LHS 271, 6.53 $\pm$ 0.10 pc), GJ 1068 (LHS 22, 6.97 $\pm$ 0.09 pc), and GJ 1123 (LHS 263, 9.02 $\pm$ 0.16 pc). In addition, two red subdwarf-white dwarf pairs, LHS 193AB and LHS 300AB, are identified. The white dwarf secondaries fall in a previously uncharted region of the HR diagram.Comment: 40 pages, 7 figures, accepted to The Astronomical Journal (scheduled for April 2005 issue), Re-submit, Table 2 running off the bottom of the page has been fixe

Molecular Response of the Brain to Cross-Generational Warming in a Coral Reef Fish

Ocean warming is a threat to marine biodiversity, as it can push marine species beyond their physiological limits. Detrimental effects can occur when marine poikilotherms are exposed to conditions beyond their thermal optima. However, acclamatory mechanisms, such as plasticity, may enable compensation of detrimental effects if warming is experienced during development or across generations. Studies evaluating the molecular responses of fishes to warming have mostly focused on liver, muscle, and gonads, and little is known about the effects on other vital organs, including the brain. This study evaluated the transcriptional program of the brain in the coral reef fish Acanthochromis polyacanthus, exposed to two different warming scenarios: +1.5°C and +3.0°C, across successive generations. Fish were exposed to these conditions in both developmental (F1 and F2) and transgenerational settings (F2 only), as well as a treatment with step-wise warming between generations. The largest differences in gene expression were between individuals of the first and second generation, a pattern that was corroborated by pairwise comparisons between Control F1 and Control F2 (7,500 DEGs) fish. This large difference could be associated with parental effects, as parents of the F1 generation were collected from the wild, whereas parents of the F2 generation were reared in captivity. A general response to warming was observed at both temperatures and in developmental and transgenerational treatments included protein folding, oxygen transport (i.e., myoglobin), apoptosis and cell death, modification of cellular structure, mitochondrial activity, immunity and changes in circadian regulation. Treatments at the highest temperature showed a reduction in synaptic activity and neurotransmission, which matches previous behavioral observations in coral reef fishes. The Transgenerational +3.0°C treatment showed significant activation of the gene pls3, which is known for the development of neuro-muscular junctions under heat-stress. F2 samples exposed to step-wise warming showed an intermediate response, with few differentially expressed genes compared to developmental and transgenerational groups (except for Transgenerational +1.5°C). In combination with previous studies on liver gene expression, this study indicates that warming produces a molecular signature of stress response in A. polyacanthus that is influenced both by the intensity of warming as well as the duration of exposure

Pulmonary artery pressure and activities in chronic heart failure

AbstractLong-term continuous pulmonary artery pressure monitoring was used to investigate pressure changes during different types of exercise and normal daily activities in patients with chronic heart failure. Nine men (mean age 55 years) with treated chronic heart failure underwent continuous pulmonary artery pressure measurement with use of a micromanometer-tipped catheter with in vivo calibration and frequency-modulated recording.The mean (± SD) maximal systolic pulmonary artery pressure (in mm Hg) was 59.4 ± 26.1 on treadmill exercise, 54.9 ± 30.6 on bicycle exercise, 52.5 ± 26.1 walking up and down state and 43.5 ± 23.9 walking on a flat surface. The mean maximal diastolic pressure (in mm Hg) was 27.8 ± 14.6 on treadmill exercise, 25.5 ± 14.9 on bicycle exercise, 24.9 ± 14.8 walking up and down stairs and 20.4 ± 12.5 walking on a flat surface. The increase in pulmonary artery pressure did not correlate with the severity of the limiting symptoms except during walking on a flat surface.All patients had marked postural changes in pressure, with the systolic pressure difference from lying to standing ranging from 8 to 25 mm Hg and the diastolic pressure difference ranging from 3 to 13 mm Hg. Eating meals caused an increase in pressure in three patients, but less than that when lying flat. There was an increase in pressure during urination in four patients equal to that when walking on a flat surface. None of these activities was associated with symptoms.Neither symptoms nor pulmonary artery pressure during maximal exercise is the same as during daily activities. This may restrict the value of maximal exercise tests in assessing patients with chronic heart failure

Multi-omic Analyses of Extensively Decayed Pinus contorta Reveal Expression of a Diverse Array of Lignocellulose-Degrading Enzymes.

Fungi play a key role cycling nutrients in forest ecosystems, but the mechanisms remain uncertain. To clarify the enzymatic processes involved in wood decomposition, the metatranscriptomics and metaproteomics of extensively decayed lodgepole pine were examined by RNA sequencing (RNA-seq) and liquid chromatography-tandem mass spectrometry (LC-MS/MS), respectively. Following de novo metatranscriptome assembly, 52,011 contigs were searched for functional domains and homology to database entries. Contigs similar to basidiomycete transcripts dominated, and many of these were most closely related to ligninolytic white rot fungi or cellulolytic brown rot fungi. A diverse array of carbohydrate-active enzymes (CAZymes) representing a total of 132 families or subfamilies were identified. Among these were 672 glycoside hydrolases, including highly expressed cellulases or hemicellulases. The CAZymes also included 162 predicted redox enzymes classified within auxiliary activity (AA) families. Eighteen of these were manganese peroxidases, which are key components of ligninolytic white rot fungi. The expression of other redox enzymes supported the working of hydroquinone reduction cycles capable of generating reactive hydroxyl radicals. These have been implicated as diffusible oxidants responsible for cellulose depolymerization by brown rot fungi. Thus, enzyme diversity and the coexistence of brown and white rot fungi suggest complex interactions of fungal species and degradative strategies during the decay of lodgepole pine.IMPORTANCE The deconstruction of recalcitrant woody substrates is a central component of carbon cycling and forest health. Laboratory investigations have contributed substantially toward understanding the mechanisms employed by model wood decay fungi, but few studies have examined the physiological processes in natural environments. Herein, we identify the functional genes present in field samples of extensively decayed lodgepole pine (Pinus contorta), a major species distributed throughout the North American Rocky Mountains. The classified transcripts and proteins revealed a diverse array of oxidative and hydrolytic enzymes involved in the degradation of lignocellulose. The evidence also strongly supports simultaneous attack by fungal species employing different enzymatic strategies