Structure-activity relationships of synthetic analogs of jasmonic acid and coronatine on induction of benzo[c]phenanthridine alkaloid accumulation in Eschscholzia californica cell cultures

A facile test system based on the accumulation of benzo[c]phenanthridine alkaloids in Eschscholzia californica cell suspension culture (an indicator of defense gene activation) has been used to analyze a series of synthetic compounds for elicitor-like activity. Of the 200 jasmonic acid and coronatine analogs tested with this system, representative results obtained with 49 of them are presented here. The following can be summarized concerning structure-actvity relationships: there is a large degree of plasticity allowed at the C-3 of jasmonic acid in the activation of defense genes. The carbonyl moiety is not strictly required, but exocyclic double bond character appears necessary. The pentenyl side chain at C-2 cannot tolerate bulky groups at the terminal carbon and still be biologically active. Substitutions to the C-1' position are tolerated if they can potentially undergo beta-oxidation. Either an alkanoic acid or methyl ester is required at c-l, or a side chain that can be shortened by beta-oxidation or by peptidase hydrolysis. Coronatine and various derivatives thereof are not as effective as jasmonic acid, and derivatives in inducing benzo[c]phenanthridine alkaloid accumulation. Jasmonic acid rather than the octadecanoic precursors is therefore considered to be a likely signal transducer of defense gene activation in planta

Measuring Knowledge Management: A New Indicator of Innovation in Enterprises

This paper is part of a larger research project, which has two primary goals: (1) To develop analytic tools for examining Regional Systems of Innovation for policy makers, and (2) To identify and design new indicators of innovation and knowledge-creation in this context. It draws on the results of research conducted in a non-metropolitan region of British Columbia, in this case the Okanagan region of south-central B.C

The Link between Innovation and the Use of Human Resources in BC Enterprises

This research finds that firms serving non-metropolitan regional markets tend to have low exports, relying on suppliers and customers as sources of innovation. These firms import knowledge to a region. On the other hand, firms serving transnational markets export products or services beyond their regional or national milieu, and rely on internal R&D as a source of innovation

Innovation and the Management of Human Resources

Regional innovation clusters are the building blocks of the Canadian national system of innovation. But in the knowledge-based economy, where knowledge, embedded in the training of the human capital of the innovative firm is the primary resource for the innovative firms. This paper addresses the question: What is the relationship between the innovative behavior of the firm and the way it manages its human resources

Characteristics of Innovation in a Non-Metropolitan Area: The Okanagan Valley of British Columbia

This paper addresses the characteristics of innovation in industrial clusters in a Non-Metropolitan area of British Columbia. The Okanagan houses strong high technology, agrifood, forest products, and construction sectors. These sectors were surveyed for common characteristics indicative of a strong industrial cluster

Scaffolding Classroom Discourse in an Election Year: Keeping a Cool Mood in a Heated Season

Certainly, teaching about politics can be daunting, especially as the political climate in the United States becomes increasingly partisan as a result of heated political rhetoric amplified through a variety of media outlets. However, elementary teachers can help students develop the respectful dispositions they will need as young adults living in an increasingly pluralistic society. These dispositions can be fostered only if teachers are willing to engage their young learners in discussions of politics as part of the curriculum. In this article, the authors discuss ways in which teachers can promote political tolerance and respect during coverage of a presidential election, a high-profile event that social studies educators Mary Haas and Margaret Laughlin describe as the quintessential example of teaching social studies. Here, the authors also describe instructional strategies designed specifically for the upper elementary grades, although they note ways in which teachers can adapt these strategies for younger learners as well

Science lives: School choices and ‘natural tendencies’

An analysis of 12 semi-structured interviews with university-based scientists and non-scientists illustrates their life journeys towards, or away from, science and the strengths and impact of life occurrences leading them to choose science or non-science professions. We have adopted narrative approaches and used Mezirow's transformative learning theory framework. The areas of discussion from the result have stressed on three main categories that include ‘smooth transition’, ‘incremental wavering transition' and ‘transformative transition’. The article concludes by discussing the key influences that shaped initial attitudes and direction in these people through natural inclination, environmental inspirations and perceptions of science

OX133, a monoclonal antibody recognizing protein-bound N-ethylmaleimide for the identification of reduced disulfide bonds in proteins

In vivo, enzymatic reduction of some protein disulfide bonds, allosteric disulfide bonds, provides an important level of structural and functional regulation. The free cysteine residues generated can be labeled by maleimide reagents, including biotin derivatives, allowing the reduced protein to be detected or purified. During the screening of monoclonal antibodies for those specific for the reduced forms of proteins, we isolated OX133, a unique antibody that recognizes polypeptide resident, N-ethylmaleimide (NEM)-modified cysteine residues in a sequence-independent manner. OX133 offers an alternative to biotin-maleimide reagents for labeling reduced/alkylated antigens and capturing reduced/alkylated proteins with the advantage that NEM-modified proteins are more easily detected in mass spectrometry, and may be more easily recovered than is the case following capture with biotin based reagents

Clothing Longevity Protocol: final

The Clothing Longevity Protocol offers guidelines for good practice in order to aid moves towards garments that will last longer and thus to help protect brand value, screen out garments which fail prematurely and reduce the environmental impact of the clothing sector