Mapping QTLs related to yield and yield components under drought in bread wheat

The case-control study is an efficient study design for evaluating the effect of cancer screening. This study method enables calculation of the percentage by which the risk of mortality from cancer decreases in participants in a screening programme if the carcinoma is detected early. Reductions in mortality of 15-65% have been published for participants in a population screening programme for breast, cervical and colorectal cancer. The case-control study is an observational study, in other words it does not have an experimental design. There is, therefore, a risk that confounding and self-selection bias may cause over- or underestimation of the mortality reduction. It is, therefore, important that at publication investigators indicate the extent to which they have succeeded in minimalising the occurrence of these forms of bias in their study design and data analysis

Selection of Specific Single Chain Variable Fragments (SCFV) Against Polymyxa Betae from Phage Display Libraries

Sugar beet is one of the most important industrial crops in Iran. For the last two decades it has been mainly affected by a destructive virus, beet necrotic yellow vein virus (BNYVV). The Polymyxa betae is the only natural transmitting agent of the disease among the plants. Developing accurate diagnostic methods may have a major impact on the rising of resistant germplasms. In the present study, specific monoclonal recombinant antibodies in the form of single chain variable fragments (scFv) were obtained from naïve phage display libraries. The fungus specific glutathione-S-transferase (GST) protein was chosen as an antigen for developing antibodies and diagnostic purposes. To generate specific scFv, screening of Tomlinson phage display libraries was performed by applying both recombinant and native fungal GST. Using the recombinant GST in the panning process resulted in the isolation of an antibody only bound to recombinant GST but it failed to detect native GST in the infected plants. Alternatively, the process of panning was carried out by applying native fungal GST trapped to immunotubes through specific polyclonal antibody intermediate. The recent approach resulted in the selection of a specific scFv binding to native GST which was able to detect the presence of the fungi within infected plants. To the best of our knowledge, this is the first report on the generation of recombinant antibodies against Polymyxa betae, fungal vector of sugar beet rhizomania disease