Determination of cisplatin in human blood plasma and urine using liquid chromatography-mass spectrometry for oncological patient s with a variety of fatty tissue mass for prediction of toxicity

Publisher Copyright: Copyright © Experimental Oncology, 2017.Aim: The research was aimed to analyze a level of triglycerides in blood serum as a possible new marker of toxicity, particularly in patients with excess body weight, receiving cisplatin. Materials and Methods: Study involved 20 oncological patients with stage III lung cancer, who received palliative treatment with cisplatin. High-performance liquid chromatography was used for quantitative determination of pure cisplatin in urine and blood samples. Cisplatin concentration of the test samples was determined based on the data obtained from the calibration graph. Results: Quantitative determination of pure cisplatin is quite complicated. The elimination half-time for one of the groups was observed higher almost by half than for other patients. Higher dose of cisplatin showed a significant association with increase in triglyceride levels. We found a close correlation between body mass index and triglyceride changes during chemotherapy (p = 0.001; r = 0.67). The results indicate that a higher body mass index gives higher fluctuations of triglyceride levels in blood serum. Analyses of correlation between level of triglycerides and elimination half-time show that by an increase in the level of triglycerides in the blood serum cisplatin elimination half-time is prolonged (R2 Linear = 0.596). Cisplatin concentration in urine is higher and elimination takes longer time at elevated levels of triglycerides, where close correlation between fraction of excreted substance in urine and concentration parameters was seen (p < 0.01). Also good correlation for body mass index with fraction of excreted substance in urine and concentration parameters was observed (p < 0.05). Conclusion: Clearance of cisplatin, which was determined by the chromatographic method, is reduced in individuals with increased adipose tissue mass. Research data suggest that overweight affects cisplatin elimination from the body. The greater body fat mass can contribute to a greater rise of triglyceride level in blood serum. Triglycerides in blood plasma may serve as an additional indicator of higher cisplatin toxicity as a cardiotoxicity marker.publishersversionPeer reviewe

Determination of cisplatin in human blood plasma and urine using liquid chromatography-mass spectrometry for oncological patients with a variety of fatty tissue mass for prediction of toxicity

Aim: The research was aimed to analyze a level of triglycerides in blood serum as a possible new marker of toxicity, particularly in patients with excess body weight, receiving cisplatin. Materials and Methods: Study involved 20 oncological patients with stage III lung cancer, who received palliative treatment with cisplatin. High-performance liquid chromatography was used for quantitative determination of pure cisplatin in urine and blood samples. Cisplatin concentration of the test samples was determined based on the data obtained from the calibration graph. Results: Quantitative determination of pure cisplatin is quite complicated. The elimination half-time for one of the groups was observed higher almost by half than for other patients. Higher dose of cisplatin showed a significant association with increase in triglyceride levels. We found a close correlation between body mass index and triglyceride changes during chemotherapy (p = 0.001; r = 0.67). The results indicate that a higher body mass index gives higher fluctuations of triglyceride levels in blood serum. Analyses of correlation between level of triglycerides and elimination half-time show that by an increase in the level of triglycerides in the blood serum cisplatin elimination half-time is prolonged (R²Linear = 0.596). Cisplatin concentration in urine is higher and elimination takes longer time at elevated levels of triglycerides, where close correlation between fraction of excreted substance in urine and concentration parameters was seen (p < 0.01). Also good correlation for body mass index with fraction of excreted substance in urine and concentration parameters was observed (p < 0.05). Conclusion: Clearance of cisplatin, which was determined by the chromatographic method, is reduced in individuals with increased adipose tissue mass. Research data suggest that overweight affects cisplatin elimination from the body. The greater body fat mass can contribute to a greater rise of triglyceride level in blood serum. Triglycerides in blood plasma may serve as an additional indicator of higher cisplatin toxicity as a cardiotoxicity marker

Early literacy skills in Latvian preschool children with specific language impairment

The present study explores differences in early literacy skills of Latvian preschool children with Specific Language Impairment (SLI) compared to children from general population. The participants were 21 children with diagnosis of Specific developmental disorders of speech and language (F80; ICD-10) and 21 children as matched control group (in each group: mean age=79 months, 88% boys). Both samples were selected from the adaptation and standardization study of Dynamic Indicators of Basic Early Literacy Skills (DIBELS Next) in Latvia (Good & Kaminski et al., 2011; Latvian version, Rascevska et al., 2013a). The results show significant differences between two groups in DIBELS Next composite score (t=3.09, p<.01), First Sound Fluency (t = 2.54, p<.05), Phoneme Segmentation Fluency (t = 2.80, p<.01), Correct Letter Sounds (t = 3.38, p<.01) and Whole Words Read (t = 3.39, p<.01) from Nonsense Word Fluency. Phoneme awareness represented by first sound and phoneme segmentation fluency and phonological decoding observed during nonsense word reading was poorer for the SLI group, albeit letter naming did not differ in both groups. No differences in letter naming might be explained due to intensive instruction the children with SLI are receiving in their institution of special education, while children from general population might not have this enhanced support

Time perspective profiles of cultures

This chapter summarises some results of the International Time Perspective Research Project, which is a collaborative cross-cultural study of time perspective carried out in 24 countries. The highlights of structural equivalence assessment study are presented, showing the cross-cultural invariance of 36 items of the Zimbardo Time Perspective Inventory (ZTPI) scale. The associations between country-level ZTPI scores and other culture-level indicators, including the Human Development Index and Hofstede cultural dimensions, are presented and discussed. Using hierarchical cluster analysis, five distinct profiles of time perspective were found (future-oriented, present-oriented, balanced, moderately fatalistic, and negative), and significant differences in the prevalence of these profiles across cultures were found. Implications and perspectives for future research are discussed