Toward a typology for social-ecological systems

Characterizing and understanding social-ecological systems (SESs) is increasingly necessary to answer questions about the development of sustainable human settlements. To date, much of the literature on SES analysis has focused on "neat" systems involving a single type of resource, a group of users, and a governance system. While these studies provide valuable and specific insights, they are of limited use for application to "messy" SESs that encompass the totality of human settlements, including social organization and technologies that result in the movement of materials, energy, water, and people. These considerations, in turn, create distribution systems that lead to different types of SESs. In messy SESs the concept of resilience, or the ability of a system to withstand perturbation while maintaining function, is further evolved to posit that different settlements will require different approaches to foster resilience. This article introduces a typology for refining SESs to improve short- and long-term adaptive strategies in developing human settlements

A haploid pseudo-chromosome genome assembly for a keystone sagebrush species of western North American rangelands

Increased ecological disturbances, species invasions, and climate change are creating severe conservation problems for several plant species that are widespread and foundational. Understanding the genetic diversity of these species and how it relates to adaptation to these stressors are necessary for guiding conservation and restoration efforts. This need is particularly acute for big sagebrush (Artemisia tridentata; Asteraceae), which was once the dominant shrub over 1,000,000 km2 in western North America but has since retracted by half and thus has become the target of one of the largest restoration seeding efforts globally. Here, we present the first reference-quality genome assembly for an ecologically important subspecies of big sagebrush (A. tridentata subsp. tridentata) based on short and long reads, as well as chromatin proximity ligation data analyzed using the HiRise pipeline. The final 4.2-Gb assembly consists of 5,492 scaffolds, with nine pseudo-chromosomal scaffolds (nine scaffolds comprising at least 90% of the assembled genome; n = 9). The assembly contains an estimated 43,377 genes based on ab initio gene discovery and transcriptional data analyzed using the MAKER pipeline, with 91.37% of BUSCOs being completely assembled. The final assembly was highly repetitive, with repeat elements comprising 77.99% of the genome, making the Artemisia tridentata subsp. tridentata genome one of the most highly repetitive plant genomes to be sequenced and assembled. This genome assembly advances studies on plant adaptation to drought and heat stress and provides a valuable tool for future genomic research.This research was made possible by 2 NSF Idaho EPSCoR grants (award numbers OIA-1757324 and OIA-1826801), as well as a Dovetail Genomics Tree of Life Award.Introduction Materials and methods Sample collection, in vitro tissue propagation, and biomass production Flow cytometry and genome complexity analysis PacBio and Omni-C sequence data generation PacBio long-read de novo assembly and validation Pseudomolecule construction with HiRise Genome annotation RNA sequencing Repeat identification Functional annotation Results and discussion Validation of genome assembly and annotation Genome complexity and evidence of past polyploidization Comparing the A. tridentata and A. annua genome assemblies Applications of the sagebrush reference genome Data availability Acknowledgments Literature cite

A Typology for Complex Social-Ecological Systems in Mountain Communities

The efficient assessment of social-ecological systems is crucial for supporting increased resilience of human communities. One challenge for researchers and managers is that few frameworks exist to assess social-ecological resilience and vulnerability for different landscapes. Mountain landscapes offer management challenges because of their valuable ecosystem services, varying biophysical characteristics, steep gradients, and the socioeconomics of human communities that are distributed across watersheds. Despite this, many mountain communities and regions lack effective management plans to enable adaptive responses to changing socioeconomic and environmental conditions. Assessing the vulnerability and resilience of mountain communities in a standardized manner is an important step in developing adaptation strategies. To help fill this gap, we investigated the utility of a conceptual social-ecological systems typology by assessing 21 mountain communities in the western United States. Our results show that larger cities or urban areas are generally more resilient than smaller communities, but the difference does not seem to be significant. Where there are resilience differences they are often found between communities of different population sizes. However, no community in the Western United States is seen as highly vulnerable to social-ecological change. More broadly, development of such standardized social-ecological systems typologies that are region-specific can be applied towards accommodating other unique environmental niches, while allowing for cross comparisons between regions on a broader continental scale

Osteoclast-like cells in soft tissue leiomyosarcomas.

Giant cell-rich leiomyosarcoma of soft tissues is an unusual variant of malignant smooth muscle tumor characterized by the presence of numerous multinucleated giant cells (MNGCs). The nature of MNGCs and the cellular mechanisms underlying their accumulation in this tumor are poorly understood. Analysis of the expression of osteoclast, macrophage, and smooth muscle markers in two cases of giant cell-rich leiomyosarcoma revealed that the MNGCs in giant cell-rich leiomyosarcoma were negative for smooth muscle markers and that these cells expressed an osteoclast-like phenotype, being positive for CD45, CD68, tartrate-resistant acid phosphatase, and CD51 but negative for CD14 and HLA-DR. Scattered tumor-associated macrophages (TAMs) also expressed this phenotype. Leiomyosarcoma tumor cells strongly reacted for CD51 but were negative for CD14, CD45, and CD68. An analysis of 25 conventional (nongiant cell-containing) leiomyosarcomas found isolated CD68(+) MNGCs in three cases (12%), all of which were grade II/III leiomyosarcomas containing a prominent TAM infiltrate. Leiomyosarcoma-derived TAMs in the presence of receptor activator for nuclear factor kappa B ligand (RANKL) and macrophage colony-stimulating factor were capable of differentiating into osteoclast-like cells capable of resorbing bone. Reverse transcription polymerase chain reaction studies showed that RANKL, osteoprotegerin, and TNF-related apoptosis-inducing ligand were expressed by leiomyosarcoma cells. Our findings indicate that the giant cells found in leiomyosarcomas are osteoclast-like and that they are formed from TAMs by a RANKL-dependent mechanism

Best Practices Integrating Social Sciences into Social Ecological Systems Science: Future Directions for Building a More Resilient America

There are three purposes to this Best Practices Report: 1. Outline potential pathways to establish best practices in integrating the social sciences into social ecological systems (SES) science; 2. To provide a framework and actions that can be vetted, refined and used nationally; 3. To rapidly, but systematically, advance social ecological systems science to the point where it can be more readily applied to pressing management needs, from local to global scales