Prevalence and characterization of Salmonella and Listeria monocytogenes in french raw pork meat at the distribution level

This study was undertaken in 2010 to estimate the occurrence of Listeria monocytogenes in raw pork meat at the distribution level in France

Comparison of organic and conventional pig productions on prevalence, antibiotic resistance and genetic diversity of Escherichia coli

The objectives of this study were to assess the prevalence, the tetracycline resistance level and the genetic diversity of Escherichia coli isolated from organic pigs in comparison with conventional pigs. This study is integrated in a global European project, Safeorganic, funded trough CORE Organic II call. 25 organic and 25 conventional herds were considered in one slaughterhouse from April to October 2012. Colon content of 2 pigs per herds was sampled. For each pig, enumeration of E. coli and of tetracycline resistant E. coli (TET+E. coli) was determined

Antimicrobial susceptibility of S. aureus strains isolated from a ham plant in France

As MRSA (methicillin resistant Staphylococcus aureus) were recently isolated from pigs in the Netherlands (1), we were incited to evaluate the resistance gene circulation inside S. aureus strains isolated from swine food products. A study, build in 2002, established the biodiversity of S. aureus isolates originated from a single raw ham industrial unit located in western France. Biotypes and pulsotypes from 106 isolates from raw material ham, end product and plant environment were determined. Isolates split up into 7 biotypes and about forty pulsotypes, which delineated a total of 23 sub-groups with 80% of homology and indicated the presence of numerous S. aureus strains in the plant

Data on distribution and characterization of Listeria monocytogenes strains in a pork slaughter and cutting plant in Quebec support an earlier surveillance in the meat production chain

Listeria monocytogenes is a major public health concern. It has been associated with highly mediatised outbreaks. It was the case in 2008, when an important episode (23 deaths) in Canada showed the need to reinforce the policies to better control Listeria in ready-to-eat products. Improved surveillance in the production plants environment increased the detection levels and represents a challenge for both industries and control agencies

Comparison of PFGE and antibioresistance profiles of Salmonella enterica serotype Derby isolated from pig and human salmonellosis in France

In France, Salmonella enterica serotypes Typhimurium and Derby are the most often isolated serotypes in pigs. On the other hand, serotype Derby ranks usually between 3rd and 4th in prevalence among human serotyped isolates in France. In 2006 and 2007, 150 and 127 human isolates were respectively collected by the French National Reference Center for Salmonella. The aim of this study was to evaluate the similarity between human and pig serotype Derby isolates on both their Pulsed Field Gel Electrophoresis (PFGE) patterns after Xbai restriction and on their antimicrobial resistance profiles

Comparison of organic and conventional pig productions on prevalence, antibiotic resistance and genetic diversity of Escherichia coli

The objectives of this study were to assess the prevalence, the tetracycline resistance level and the genetic diversity of Escherichia coli isolated from organic pigs in comparison with conventional pigs. This study is integrated in a global European project, Safeorganic, funded trough CORE Organic II call. 25 organic and 25 conventional herds were considered in one slaughterhouse from April to October 2012. Colon content of 2 pigs per herds was sampled. For each pig, enumeration of E. coli and of tetracycline resistant E. coli (TET+E. coli) was determined.</p

Prevalence of low-virulence Listeria monocytogenes strains from different foods and environments

International audienceVarious studies have demonstrated variations in the levels of virulence of different L monocytogenes strains. In our laboratory, a plaque-forming assay followed by subcutaneous footpad inoculation of mice enabled us to estimate the prevalence of the low-virulence strains. This value fell from 16.3% to 1.7% with bacteria collected before 1994 and after 1997 respectively. This could be related to the modification in 1997 of the reference method EN ISO 11 290-1 of Listeria detection which recommended the use of polymyxin-acriflavine-LiCl-ceftazidime-aesculin-mannitol (PALCAM) medium. The aim of this study was to determine whether the percentage of low-virulence strains detected has changed due to the modification of the detection method recommending the use of the ALOA medium. After analyzing 380 L monocytogenes strains, no increase in the percentage of low-virulence strains could be detected. The prevalence reached only 2.6% (ten of the 380 strains tested). The low virulence of L monocytogenes strains was not related to rare serotypes and was also observed in serotypes usually involved in human disease. Low-virulence strains were found in dairy, meat, ready-to-eat products and also in the environment. highlighting the absence of one specific source. These results are discussed in terms of detection methods and the definition of low virulence. (C) 2009 Elsevier B.V. All rights reserved

Antimicrobial susceptibility of S. aureus strains isolated from a ham plant in France

As MRSA (methicillin resistant Staphylococcus aureus) were recently isolated from pigs in the Netherlands (1), we were incited to evaluate the resistance gene circulation inside S. aureus strains isolated from swine food products. A study, build in 2002, established the biodiversity of S. aureus isolates originated from a single raw ham industrial unit located in western France. Biotypes and pulsotypes from 106 isolates from raw material ham, end product and plant environment were determined. Isolates split up into 7 biotypes and about forty pulsotypes, which delineated a total of 23 sub-groups with 80% of homology and indicated the presence of numerous S. aureus strains in the plant.</p