Simultaneous quantification of actin monomer and filament dynamics with modelling assisted analysis of photoactivation

Photoactivation allows one to pulse-label molecules and obtain quantitative data about their behavior. We have devised a new modeling-based analysis for photoactivatable actin experiments that simultaneously measures properties of monomeric and filamentous actin in a three-dimensional cellular environment. We use this method to determine differences in the dynamic behavior of β- and γ-actin isoforms, showing that both inhabit filaments that depolymerize at equal rates but that β-actin exists in a higher monomer-to-filament ratio. We also demonstrate that cofilin (cofilin 1) equally accelerates depolymerization of filaments made from both isoforms, but is only required to maintain the β-actin monomer pool. Finally, we used modeling-based analysis to assess actin dynamics in axon-like projections of differentiating neuroblastoma cells, showing that the actin monomer concentration is significantly depleted as the axon develops. Importantly, these results would not have been obtained using traditional half-time analysis. Given that parameters of the publicly available modeling platform can be adjusted to suit the experimental system of the user, this method can easily be used to quantify actin dynamics in many different cell types and subcellular compartments

Management of patent ductus arteriosus in premature infants.

Closure of hemodynamically significant patent ductus arterios (HSPDA)  is one of the most important questions in modern neonatal intensive care, especially for preterm babies. Long-term functioning of the hemodynamically significant  arterial duct leads to a large number of complications in premature babies, such as: bronchopulmonary dysplasia, periventricular leucomalacia, intraventricular hemorrhage, retinopathy of the premature. To prevent all these complications, the PDA should be closed pharmacologically or surgically as soon as possible without any hesitation. COX inhibitors are commonly used nowa days. Ibuprofen and indomethacin show the equal efficacy and no significant adverse events. But some patients still need surgical treatment. The aim of the study was to determine the feasibility, effectiveness and safety of using various volumes of infusion in combination with COX inhibitors and to determine its effect on the timing of the closure of PDA. 91 premature infants with a gestational age of 26-31 weeks with manifestations of respiratory distress syndrome and НSPDA were studied retrospectively. Premies were divided into 2 groups. Research groups were representative as to gestational age, gender, and weight (1205.0±435.0 g). Therapy for PDA closure included the use of various volumes of restrictive or liberal infusion therapy (from 50 to 100 ml/kg/day) and COX inhibitors (indomethacin, ibuprofen). The volume of infusion therapy was limited in the first group. Preemies received 53.5±6.4 ml/kg/day on DOL1 and 2. From the third day urine excretion increased and the volume of infusion therapy also raised to 63.6±5.6 ml/kg/day, and on day 5 – to 89.7±6.8 ml/kg/day. In the second group there was no strict limitation of the volume of infusion therapy (especially in the first 5 days). Delayed period of PDA closure (on average from 14.55±0.56 DOL) was associated with absence of limitation of the infusion volume. In the first group, volume of infusion therapy was restricted in the first 5 days, and the closure of the ductus arteriosus occurred extremely early (on 2.35±0.48 DOL). COX inhibitors were prescribed according to the standard scheme: in the first 3 days indomethacin was administered orally in doses of 0.2/0.1/0.1 mg/kg/day. If the premature baby had symptoms of intestinal paresis ( this restricted oral administration of indomethacin), ibuprofen was prescribed in a three-day course in doses of 10/5/5 mg/kg/day intravenously or 20/10/10 mg/kg/day in rectal form. In all  groups, standard PDA closure therapy was used. In the more remote periods (14 and 28 days), there was no fundamental difference in the volume of infusion in all groups. For early PDA closure limitation of infusion therapy in the first 3-5 days in combination with COX is principle

Organization of medical aid with for the population with diseases of upper respiratory tract associated with chlamydia infection

At the same time optima organization and. measures of treatment of extra-urogenital forms of chlamydiosis aren't developed yet and. this problem is needed to be solved. The article presents scientific basis of necessity of developement of complex of measures and. main directions that contribute to the organization of medical aid. for the people with infections of upper respiratory tract associated with chlamydiosis. 1827 people were examineds. First group included. l8—60 years old. employees of several enterprises (846 people) and. 3—17 years old. children attending kindergarten and school (483 childern). Second group included patients of ENT-ward of institute's clinics with, inflammatory pathology of upper respiratory tract (498 people) of 3—60 years old. including 246 childern and. 252 adults. Programme of optimization of medical aid. for people with chlamydia infection of upper respiratory tract that included complex of measures with directions divided into 4 main parts (organization and. performing, information. and. analytics, treatment, diagnostics and. prophylactics, study and. methodology). This programme can be the basis of development of system of medical aid for people with respiratory chlamydiosis by public health service agencie of Russian Federation subjects that contributed to the increase of effectiveness of treatment, diagnostic and. preventive measured. Algrorhythms of diagnostics developed by the authors are offered to use as diagnostic standards of chlamydia infection of upper respiratory tract for phycisians of different duties (otolaryngologists, therapeutists, pediatritians, phycisians of common duty, infectionists etc.)

МУЗЕЙ ИНСТИТУТА СКОРОЙ ПОМОЩИ ИМ. Н.В. СКЛИФОСОВСКОГО: ОТ ЮДИНА ДО НАШИХ ДНЕЙ

ABSTRACT. The history of the N.V. Sklifosovsky Research Institute for Emergency Medicine has been studied to divide it into periods and characterize them. It was shown that the stages of the Museum existence coincide with the periods of the Institute history. So called “Period of Yudin” can be identified separately. The great contribution of the Institute’s Surgeon-in-chief prof. S. S. Yudin to the foundation of the Museum is emphasized, as well as transformation of the museum of the history of surgery with its concept and collections into the museum of the multidisciplinary medical institution, which represents the background and achievements of the Institute. РЕЗЮМЕ. Изучена история становления музея НИИ скорой помощи им. Н.В. Склифосовского с целью ее периодизации и характеристики каждого периода. Показано, что этапы существования музея совпадают с периодизацией истории института. Отдельно можно выделить Юдинский период. Подчеркнут выдающийся вклад главного хирурга Института профессора С.С. Юдина в создание музея, представлена трансформация его концепции и содержания коллекций от музея истории хирургии к музею Института, конкретного отражающего его специфику и достижения как многопрофильного медицинского учреждения.

Computer support of interdisciplinary communication of analytic geometry and algebra

© 2015 by iSER, International Society of Educational Research. The relevance of the research is due to the modernization of higher mathematical education in Russia, which led to a significant change in the curriculum and the need to establish new relationships of disciplines. The aim of the article is to find ways to solve the problem of the interdisciplinary connections in the teaching of present course “Analytical geometry” and “Algebra” in higher education. The leading method of the study of this problem is the methodical analysis and subsequent synthesis, which allows, having analyzed the didactic content of the "Analytical Geometry" and "Algebra"courses, to identify the necessary interdisciplinary communication between them and find ways to implement them through the use of modern educational technologies. It is proved that one of the instruments for implementing these methods is a computer, in particular systems of computer mathematics (Maple, Mathematica). Article submissions may be useful to teachers of mathematical disciplines of higher education institutions

Left ventricular global function index: diagnostic and prognostic value in cardiovascular diseases

Left ventricular global function index (LVGFI) is a novel indicator for assessing LV function, considering the main components of cardiac remodeling, obtained using magnetic resonance imaging and echocardiography. Works with the assessment of normal LVGFI values were analyzed. The review provides data on the diagnostic and prognostic efficacy of LVGFI in various cardiovascular diseases, such as heart failure, myocardial infarction, cardiomyopathy, and amyloidosis. Examples of LVGFI calculation in healthy patients and in those with listed pathologies are also presented