Development of a new model tool for evaluating groundwater resources within the Floridan Aquifer System in Southern Florida, USA

The US Army Corps of Engineers (USACE) and the South Florida Water Management District (SFWMD) are partners in an ambitious plan to restore water flows throughout the Everglades ecosystem. An important component of the restoration plan involves storing excess stormwater deep underground in the Floridan Aquifer System using aquifer, storage and recovery (ASR) wells. In order to determine the optimal ASR system and to assess environmental impacts, USACE spent over 11 years and significant resources to develop a three-dimensional groundwater model of the Floridan Aquifer System covering a large portion of the Florida peninsula. This SEAWAT model is capable of evaluating changes in aquifer pressures and density-dependent flows in the entire study area. The model has been used to evaluate the Everglades ASR system already but could also be used by water managers for other important water resources studies in Florida including water supply estimates and adaptation to climate change. As part of an effort to make the model more readily available for other important studies, this study documents and summarizes the overall development of the SEAWAT model including a discussion regarding the intensive calibration and validation efforts undertaken during model development. The paper then demonstrates the use of the model using Everglades ASR project alternatives. Lastly, the paper outlines potential future uses of the model along with its overall limitations. Supplementary online resources are also included that provide researchers with further detail regarding the model development effort beyond the scope of this summary article as well as model development databases

Optimizing microarray in experimental hypertension

Optimizing microarray in experimental hypertension.BackgroundGenetic noise between outbred animals can potentially be a major confounder in the use of microarray technology for gene expression profiling. The study of paired organs from the same animal offers an alternative approach (e.g., for studies of the kidney in experimental hypertension). The present study was undertaken to determine the level of genetic noise between outbred adult Sprague-Dawley (SD) rats, and to determine the effects of unilateral nephrectomy on changes in gene expression as a basis for the design of microarray studies in experimental hypertension.MethodsMale SD rats (approximately 130g) were acclimatized before measurement of tail-cuff systolic blood pressure (SBP) for 6 control days and 4 days of saline treatment. Left kidney nephrectomy was performed, and the tissue snap-frozen in liquid nitrogen for subsequent RNA extraction. Two weeks later, SBP was measured over 4 control and 8 saline treatment days, and the remaining right kidney removed and frozen. Total RNA purification, preparation of cRNA, hybridization, and scanning of the Rat U34A Affymetrix arrays were performed, and data analyzed using MAS5 software Affymetrix Suite (v5), Bioconductor, as well as statistical methods motivated by relevant simulations.ResultsGene expression profiles in the left control kidney were extremely consistent across animals. The expression profiles of pairs of kidneys from the same animal were, however, more similar than those of kidneys from different animals. Nephrectomy had little effect on the gene expression profiles in the time frame examined.ConclusionDespite the outbred nature of the rats used in this study, they are useful for gene expression profiling comparisons. The use of paired organs from an individual animal ensures even further genetic identity, allowing determination of genes modified by the treatment of interest