Spectrofluorimetric determination of sertraline in dosage forms and human plasma through derivatization with 9-fluorenylmethyl chloroformate

<p>Abstract</p> <p>Background</p> <p>Sertraline is primarily used to treat major depression in adult outpatients as well as obsessive-compulsive, panic and social anxiety disorders in both adults and children. A survey of the literature reveals that most of the reported methods are either insufficiently sensitive or tedious and require highly sophisticated and dedicated instrumentation. The proposed method is considered to be specific for determination of SER in presence of its metabolite (deaminated form).</p> <p>Results</p> <p>A sensitive, simple and specific spectrofluorimetric method was developed for the determination of sertraline (SER) in pharmaceutical formulations and biological fluids. The method is based on its reaction with 9-fluorenylmethyl chloroformate (FMOC-Cl) in borate buffer of pH 8.0 to yield a highly fluorescent derivative peaking at 315 nm after excitation at 265 nm. The different experimental parameters affecting the development and stability of the reaction product were carefully studied and optimized. The fluorescence concentration plot was rectilinear over the range of 0.05-1.0 μg mL^-1with a lower detection limit of 5.34 × 10^-3μg mL^-1and limit of quantitation of 0.016 μg mL^-1.</p> <p>Conclusions</p> <p>The proposed method was successfully applied to the analysis of commercial tablets and the results obtained were in good agreement with those obtained using the reference method. Furthermore, the method was applied for the determination of SER in spiked and real human plasma. The mean % recovery (n = 3) was 94.33 ± 1.53 and 92.00 ± 2.65, respectively. A proposal of the reaction pathway was postulated.</p

Multi-parameter optimization of a loose focusing high flux high-harmonic beamline

We perform a multi-dimensional parameter scan in the generation of high-order harmonics, with the main purpose to find the macroscopic conditions that optimize the harmonic yield in a specific spectral domain, around 40 eV for this particular case. The scanned parameters are the laser pulse energy, gas pressure, interaction cell position relative to focus and the cell length, while the fixed parameters are chosen to model a loose focusing configuration which is used in many existing laboratories. We performed the simulations with a 3D non-adiabatic model complemented by a detailed analysis of the phase matching mechanisms involved in an efficient harmonic generation. Based on the results we identify a range of parameter combinations that lead to a high yield in the specified spectral domain. The method and results presented here can be the framework for the design and construction of high flux high-order harmonic generation beamlines