The onset of grasses in the Amazon drainage basin, evidence from the fossil record

Poaceae (the grass family) originated in the Cretaceous, but first dominate the palynological records of the Amazon drainage basin (ADB) in the Neogene (23 to 2.5 million years ago (Ma)). However, the ecological role of grasses in the landscape during this time remains to be resolved. In this paper, we summarise the global significance of grasses and the relevance of the fossil record, and evaluate the history of the grasses in the ADB. We present a 3-stage model of the changing role of grasses, which we based on a revision of Neogene depositional environments, the palynological record, and modern grass distribution in the Neotropics. Our model comprises the following hypotheses: (H1) assumes that from c. 23 to 9 Ma western Amazonia was dominated by a megawetland (the ‘Pebas system’) that harboured large amounts of (aquatic?) grasses. In (H2) we propose that from c. 9 Ma Andean uplift prompted megafans (extremely large alluvial fans) that extended from the Andes into the lowlands. Meanwhile, the ‘Pebas’ megawetland gradually transformed into a fluvial system. In this scenario, grasses would have had a competitive advantage and were able to colonise the newly formed megafan and fluvial landscapes. Finally, in (H3) we suggest that landscape dynamics and climatic change intensified from c. 3.5 Ma, allowing for a renewed expansion of the grasses. In addition, both the fossil and molecular records suggest that from c. 5 Ma grasses were firmly established in the tropical alpine vegetation (páramo), the tropical lowland floodplains (várzeas), and savannas (cerrado). Although further study will have to confirm the precise nature of the ADB grass history, we anticipate that abiotic processes during the Neogene and Quaternary left a strong imprint in the grass phytogeography of northern South America

Perceptions of healthcare robots as a function of emotion-based coping:The importance of coping appraisals and coping strategies

The urgent pressure on healthcare increases the need for understanding how new technology such as social robots may offer solutions. Many healthcare situations are emotionally charged, which likely affects people's perceptions of such robots in healthcare contexts. Thus far however, little attention has been paid to how people's prior emotions may influence their perceptions of the robot. Based on emotional appraisal theories and prior research, we assume that particularly emotional coping appraisals would influence healthcare-robot perceptions. Additionally, we tested effects of actual coping through the use of emotion-focused and problem-focused coping strategies. Hypotheses were tested in a 2 (sad vs. angry) x 2 (hard-to-cope-with vs. easy-to-cope-with) between-subjects experiment, also including a control group. Results (N = 132; age range 18–36) showed that manipulated coping potential indirectly affected perceptions of a healthcare robot via the appraisal of coping potential. Furthermore, positive emotion-focused coping affected perceptions of a healthcare robot positively. Thus, people's healthcare-robot perceptions were affected by how they cope or how they think they can cope with their emotions, rather than by the emotions as such

Rat kinesin light chain 3 associates with spermatid mitochondria

AbstractWe recently discovered that in rat spermatids, kinesin light chain KLC3 can associate with outer dense fibers, major sperm tail components, and accumulates in the sperm midpiece. Here, we show that mitochondria isolated from rat-elongating spermatids have bound KLC3. Immunoelectron microscopy indicates that the association of KLC3 with mitochondria coincides with the stage in spermatogenesis when mitochondria move from the plasma membrane to the developing midpiece. KLC3 is able to bind in vitro to mitochondria from spermatids as well as somatic cells employing a conserved kinesin light chain motif, the tetratrico-peptide repeats. Expression of KLC3 in fibroblasts results in formation of large KLC3 clusters close to the nucleus, which also contain mitochondria: no other organelles were present in these clusters. Mitochondria are not present in KLC3 clusters after deletion of KLC3's tetratrico-peptide repeats. Our results indicate that the rat spermatid kinesin light chain KLC3 can associate with mitochondria

Interruption of visually perceived forward motion in depth evokes a cortical activation shift from spatial to intentional motor regions

Forward locomotion generates a radially expanding flow of visual motion which supports goal-directed walking. In stationary mode, wide-field visual presentation of optic flow stimuli evokes the illusion of forward self-motion. These effects illustrate an intimate relation between visual and motor processing. In the present fMRI study, we applied optic flow to identify distinct interfaces between circuitries implicated in vision and movement. The dorsal premotor cortex (PMd) was expected to contribute to wide-field forward motion flow (FFw), reflecting a pathway for externally triggered motor control. Medial prefrontal activation was expected to follow interrupted optic flow urging internally generated action. Data of 15 healthy subjects were analyzed with Statistical Parametric Mapping and confirmed this hypothesis. Right PMd activation was seen in FFw, together with activations of posterior parietal cortex, ventral V5, and the right fusiform gyms. Conjunction analysis of the transition from wide to narrow forward flow and reversed wide-field flow revealed selective dorsal medial prefrontal activation. These findings point at equivalent visuomotor transformations in locomotion and goal-directed hand movement, in which parietal-premotor circuitry is crucially implicated. Possible implications of an activation shift from spatial to intentional motor regions for understanding freezing of gait in Parkinson's disease are discussed: impaired medial prefrontal function in Parkinson's disease may reflect an insufficient internal motor drive when visual support from optic flow is reduced at the entrance of a narrow corridor. (C) 2010 Elsevier B.V. All rights reserved

Developmental Expression of the 84-kDa ODF Sperm Protein: Localization to both the Cortex and Medulla of Outer Dense Fibers and to the Connecting Piece

AbstractOuter dense fibers (ODF) are specialized cytoskeletal elements of the mammalian sperm tail which are composed of several prominent proteins. We previously reported the isolation of a cDNA (111-450) encoding a putative 84-kDa ODF protein. Here we demonstrate by independent cDNA isolations and by translational/immunoprecipitation of testicular mRNAs using anti-ODF 84 antibodies that 111-450 cDNA encodes the 84-kDa protein. We then analyzed the testicular expression of the ODF 84 mRNA and protein. Riboprobes generated from the clones recognized four testicular-specific transcripts of 1.6, 2.2, 2.4, and 2.8 kb in both rat and bull of which the immunoprecipitable product of the 2.4-kb mRNA comigrates with ODF 84 protein. Developmental Northerns indicated that the 2.2- and 2.4-kb mRNAs are first transcribed during meiotic prophase while the other two species are first expressed in round spermatids. The levels of all the transcripts steadily increased up to elongated spermatids. Immunocytochemistry revealed that the anti-84 reactive ODF proteins were synthesized and assembled in the cytoplasm of elongated spermatids (steps 9–18) with peak activity occurring in step 16 of spermiogenesis. Immunogold labeling was selective to the assembling ODF and connecting piece of the tail and to granulated bodies of the cytoplasmic lobe. Both the striated collar and capitulum of the connecting piece were immunolabeled as well as the basal plate of the implantation fossa. A combination of pre- and postembedding immunogold labeling provided evidence that the 84-kDa ODF protein is localized to both the cortex and medulla of the ODF in contrast to the sole medullary localization of the major 27-kDa ODF protein. Thus the 84-kDa ODF protein, encoded by the 2.4 transcript, is translationally regulated, packaged after synthesis into granulated bodies, assembled in a proximal to distal direction along the axoneme and may interact by means of leucine zippers specifically with the 27-kDa ODF protein during assembly. Its localization to both the cortex and medulla of the ODF, as opposed to exclusive medullary localization of the 27-kDa ODF protein, and the presence of two leucine zippers, only one of which interacts with the 27-kDa ODF, suggests that it could act as a link between proteins of the two regions of the ODF

A critical evaluation of spectrum-based fault localization techniques on a large-scale software system

In the past, spectrum-based fault localization (SBFL) techniques have been developed to pinpoint a fault location in a program given a set of failing and successful test executions. Most of the algorithms use similarity coefficients and have only been evaluated on established but small benchmark programs from the Software-artifact Infrastructure Repository (SIR). In this paper, we evaluate the feasibility of applying 33 state-of-the-art SBFL techniques to a large real-world project, namely ASPECTJ. From an initial set of 350 faulty version from the iBugs repository of ASPECTJ we manually classified 88 bugs where SBFL techniques are suitable. Notably, only 11 bugs of these bugs can be found after examining the 1000 most suspicious lines and on average 250 source code files need to be inspected per bug. Based on these results, the study showcases the limitations of current SBFL techniques on a larger program

Performance engineering for microservices and serverless applications: the RADON approach

Microservices and serverless are becoming integral parts of mod-ern cloud-based applications. Tailored performance engineering isneeded for assuring that the applications meet their requirementsfor quality attributes such as timeliness, resource efficiency, andelasticity. A novel DevOps-based framework for developing mi-croservices and serverless applications is being developed in theRADON project. RADON contributes to performance engineeringby including novel approaches for modeling, deployment optimiza-tion, testing, and runtime management. This paper summarizes thecontents of our tutorial presented at the 11th ACM/SPEC Interna-tional Conference on Performance Engineering (ICPE)