Direct characterization of circulating DNA in blood plasma using μLAS technology

Circulating cell-free DNA (cfDNA) is a powerful cancer biomarker for establishing targeted therapies or monitoring patients' treatment. However, current cfDNA characterization is severely limited by its low concentration, requiring the extensive use of amplification techniques. Here we report that the μLAS technology allows us to quantitatively characterize the size distribution of purified cfDNA in a few minutes, even when its concentration is as low as 1 pg/μL. Moreover, we show that DNA profiles can be directly measured in blood plasma with a minimal conditioning process to speed up considerably speed up the cfDNA analytical chain

Articulating learning and supporting student employability: using the concept of <i>‘illusio’</i> to make sense of the issues raised by distance learners

Universities in the UK and elsewhere are expected to demonstrate how they prepare their graduates for employment. At the same time the Widening Participation (WP) agenda for universities means that the student population is increasingly diverse and may have a pre-existing relationship with, and experience of, the world of work. Distance learners at the Open University (OU) are at the confluence of these two agendas, seeking to become graduates but with various experiences of both ‘graduate level’ and non-graduate level work. Drawing from verbal and textual data from focus groups and interviews conducted in OU regional/national centres in London and Ireland, we use Bourdieu and the concept of 'illusio', together with social and cultural capital to illustrate how these distance learners articulated their notions of employability and graduateness. We found learners developed a nuanced approach to employability as they worked to integrate their understandings of life experience and notions of ‘graduateness’. We suggest that if our aim is to both deliver learning ‘for its own sake’ and still meet the government’s employability agenda, it would be helpful for practitioners to engage in early critical conversations with learners to provide the scaffolding necessary to enable students to translate their ‘real life’ experiences into language that employers both understand and value

Sapientia, 1946, Vol. I, nº 1 (número completo)

Contenido: Editorial / La Dirección – El realismo del principio de causalidad / Reginaldo Garrigou Lagrange – La trascendencia del ser divino / Octavio Nicolás Derisi – Metafísica y lírica / Carlos A. Disandro – Notas y comentarios – Textos -- Bibliografí

THE DISPOSITION AND PHARMACOKINETICS OF ALPIDEM, A NEW ANXIOLYTIC, IN THE RAT

ABSTRACT: Th