31 research outputs found
MRSA Transmission between Cows and Humans
We isolated methicillin-resistant Staphylococcus aureus (MRSA) from cows with subclinical mastitis and from a person who worked with these animals. The bovine and human strains were indistinguishable by phenotyping and genotyping methods and were of a low frequency spa type. To our knowledge, this finding indicates the first documented case of direct transmission of MRSA between cows and humans
Q fever epidemic in Hungary, April to July 2013
We investigated a Q fever outbreak with human
patients showing high fever, respiratory tract symptoms, headache and retrosternal pain in southern
Hungary in the spring and summer of 2013. Seventy
human cases were confirmed by analysing their serum
and blood samples with micro-immunofluorescence
test and real-time PCR. The source of infection was a
merino sheep flock of 450 ewes, in which 44.6% (25/56)
seropositivity was detected by enzyme-linked immunosorbent assay. Coxiella burnetii DNA was detected
by real-time PCR in the milk of four of 20 individuals
and in two thirds (41/65) of the manure samples. The
multispacer sequence typing examination of C. burnetii DNA revealed sequence type 18 in one human
sample and two manure samples from the sheep flock.
The multilocus variable-number tandem repeat analysis pattern of the sheep and human strains were also
almost identical, 4/5-9-3-3-0-5 (Ms23-Ms24-Ms27-
Ms28-Ms33-Ms34). It is hypothesised that dried
manure and maternal fluid contaminated with C. burnetii was dispersed by the wind from the sheep farm
towards the local inhabitants. The manure was eliminated in June and the farm was disinfected in July. The
outbreak ended at the end of July 2013
Molecular detection of a putatively novel cyprinid herpesvirus in sichel (Pelecus cultratus) during a mass mortality event in Hungary
Morphological and molecular biological studies on intramuscular Myxobolus spp. of cyprinid fish
The validity of Myxobolus species infecting the skeletal muscles of six cyprinid fish species was studied by morphological and molecular biological methods. Intracellularly developing Myxobolus spores identified as M. cyprini from the common carp, M. musculi from the barbel, and M. pseudodispar from the roach, rudd, common bream and white bream were very similar in their shape and size. Nonetheless, in species identified as M. pseudodispar, the occurrence of spores with an asymmetrical shape was higher than in M. cyprini, while asymmetrical spores were only occasionally found in M. musculi. The DNA sequence analysis of the polymerase chain reaction (PCR)-amplified 18S rRNA gene of Myxobolus spores from these fish showed a similar phylogeny to that of their host species. As morphological studies and DNA sequence analysis demonstrated slight but real differences in the spores infecting muscles of the six cyprinid species, it is suggested that M. musculi, M. pseudodispar and M. cyprini are valid species